ID   CYST_PROWI              Reviewed;         255 AA.
AC   Q9TJR4;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=Probable sulfate transport system permease protein cysT;
GN   Name=cysT;
OS   Prototheca wickerhamii.
OG   Plastid; Non-photosynthetic plastid.
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Trebouxiophyceae;
OC   Chlorellales; Chlorellaceae; Prototheca.
OX   NCBI_TaxID=3111;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=263-11;
RA   Knauf U., Hachtel W.;
RT   "A 22 kb fragment of the 53 kb plastid genome of the colourless alga
RT   Prototheka wickerhamii containing atp-, rpl-,rps-, rrn-, and trn-genes.";
RL   Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Part of the ABC transporter complex cysAWTP (TC 3.A.1.6.1)
CC       involved in sulfate/thiosulfate import. Probably responsible for the
CC       translocation of the substrate across the membrane (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the binding-protein-dependent transport system
CC       permease family. CysTW subfamily. {ECO:0000305}.
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DR   EMBL; AJ245645; CAB53107.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9TJR4; -.
DR   SMR; Q9TJR4; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:InterPro.
DR   GO; GO:0042170; C:plastid membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015419; F:ABC-type sulfate transporter activity; IEA:InterPro.
DR   CDD; cd06261; TM_PBP2; 1.
DR   Gene3D; 1.10.3720.10; -; 1.
DR   InterPro; IPR011865; CysT_permease.
DR   InterPro; IPR000515; MetI-like.
DR   InterPro; IPR035906; MetI-like_sf.
DR   InterPro; IPR005667; Sulph_transpt2.
DR   PANTHER; PTHR30406; PTHR30406; 1.
DR   PANTHER; PTHR30406:SF8; PTHR30406:SF8; 1.
DR   Pfam; PF00528; BPD_transp_1; 1.
DR   SUPFAM; SSF161098; SSF161098; 1.
DR   TIGRFAMs; TIGR00969; 3a0106s02; 1.
DR   TIGRFAMs; TIGR02139; permease_CysT; 1.
DR   PROSITE; PS50928; ABC_TM1; 1.
PE   3: Inferred from homology;
KW   Membrane; Plastid; Sulfate transport; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..255
FT                   /note="Probable sulfate transport system permease protein
FT                   cysT"
FT                   /id="PRO_0000293977"
FT   TRANSMEM        2..22
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        48..68
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        77..97
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        118..138
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        167..187
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        195..215
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        225..245
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   DOMAIN          42..246
FT                   /note="ABC transmembrane type-1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
SQ   SEQUENCE   255 AA;  28208 MW;  C96588B47C5F271F CRC64;
     MLGNHLFFIP VLPLFALFSL ILKNSWKDIL EKAVDPIAIC AYGFTIKMAL IAALFNSIFG
     FLITWVITRY EFKGKKFIDA AVDLPFALPT SVAGLTLATV YGNQGWVGRF LKMGNLQIIY
     TKFGVLLAMI FVSFPFVIRS LQPVLQGLDH GLEEAAWCLG ASSFQTFLRV IFPTLVPALV
     TGFTLSFSRA LGEFGSVVMI SSNLPLDDLV TSVLIYQSLE QYDYFGASVI GAVILMIALL
     IIFLINTAQA FYSRR