CYSW_ECOLI
ID CYSW_ECOLI Reviewed; 291 AA.
AC P0AEB0; P16702; P76534;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 06-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Sulfate transport system permease protein CysW;
GN Name=cysW; OrderedLocusNames=b2423, JW2416;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=2188958; DOI=10.1128/jb.172.6.3351-3357.1990;
RA Sirko A., Hryniewicz M.M., Hulanicka D.M., Boeck A.;
RT "Sulfate and thiosulfate transport in Escherichia coli K-12: nucleotide
RT sequence and expression of the cysTWAM gene cluster.";
RL J. Bacteriol. 172:3351-3357(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT its sequence features.";
RL DNA Res. 4:91-113(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1)
CC involved in sulfate/thiosulfate import. Probably responsible for the
CC translocation of the substrate across the membrane.
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (CysA),
CC two transmembrane proteins (CysT and CysW) and a solute-binding protein
CC (CysP). {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the binding-protein-dependent transport system
CC permease family. CysTW subfamily. {ECO:0000305}.
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DR EMBL; M32101; AAA23638.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75476.2; -; Genomic_DNA.
DR EMBL; AP009048; BAA16297.1; -; Genomic_DNA.
DR PIR; F65016; QRECSW.
DR RefSeq; WP_000852686.1; NZ_STEB01000039.1.
DR RefSeq; YP_026168.2; NC_000913.3.
DR AlphaFoldDB; P0AEB0; -.
DR SMR; P0AEB0; -.
DR BioGRID; 4260746; 9.
DR ComplexPortal; CPX-4385; Sulfate/thiosulfate ABC transporter complex, cypP variant.
DR ComplexPortal; CPX-4386; Sulfate/thiosulfate ABC transporter complex, sbp variant.
DR DIP; DIP-9388N; -.
DR IntAct; P0AEB0; 2.
DR STRING; 511145.b2423; -.
DR TCDB; 3.A.1.6.1; the atp-binding cassette (abc) superfamily.
DR PaxDb; P0AEB0; -.
DR PRIDE; P0AEB0; -.
DR EnsemblBacteria; AAC75476; AAC75476; b2423.
DR EnsemblBacteria; BAA16297; BAA16297; BAA16297.
DR GeneID; 2847743; -.
DR GeneID; 66673707; -.
DR KEGG; ecj:JW2416; -.
DR KEGG; eco:b2423; -.
DR PATRIC; fig|1411691.4.peg.4308; -.
DR EchoBASE; EB0195; -.
DR eggNOG; COG4208; Bacteria.
DR HOGENOM; CLU_016047_14_0_6; -.
DR InParanoid; P0AEB0; -.
DR OMA; NEYNFVA; -.
DR PhylomeDB; P0AEB0; -.
DR BioCyc; EcoCyc:CYSW-MON; -.
DR BioCyc; MetaCyc:CYSW-MON; -.
DR PRO; PR:P0AEB0; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0035796; C:ATP-binding cassette (ABC) transporter complex, transmembrane substrate-binding subunit-containing; IC:ComplexPortal.
DR GO; GO:0016020; C:membrane; IC:ComplexPortal.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0015419; F:ABC-type sulfate transporter activity; IEA:InterPro.
DR GO; GO:1902358; P:sulfate transmembrane transport; IC:ComplexPortal.
DR GO; GO:0015709; P:thiosulfate transport; IC:ComplexPortal.
DR CDD; cd06261; TM_PBP2; 1.
DR Gene3D; 1.10.3720.10; -; 1.
DR InterPro; IPR011866; CysW_permease.
DR InterPro; IPR000515; MetI-like.
DR InterPro; IPR035906; MetI-like_sf.
DR InterPro; IPR005667; Sulph_transpt2.
DR PANTHER; PTHR30406; PTHR30406; 1.
DR PANTHER; PTHR30406:SF9; PTHR30406:SF9; 1.
DR Pfam; PF00528; BPD_transp_1; 1.
DR SUPFAM; SSF161098; SSF161098; 1.
DR TIGRFAMs; TIGR00969; 3a0106s02; 1.
DR TIGRFAMs; TIGR02140; permease_CysW; 1.
DR PROSITE; PS50928; ABC_TM1; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Sulfate transport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..291
FT /note="Sulfate transport system permease protein CysW"
FT /id="PRO_0000059997"
FT TOPO_DOM 1..22
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 23..43
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 44..69
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 70..90
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 91..104
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 105..125
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 126..141
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 142..162
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 163..200
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 201..221
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 222..247
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 248..268
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 269..291
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 66..270
FT /note="ABC transmembrane type-1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
SQ SEQUENCE 291 AA; 32538 MW; 1878BEA4EB3F6E67 CRC64;
MAEVTQLKRY DARPINWGKW FLIGIGMLVS AFILLVPMIY IFVQAFSKGL MPVLQNLADP
DMLHAIWLTV MIALIAVPVN LVFGILLAWL VTRFNFPGRQ LLLTLLDIPF AVSPVVAGLV
YLLFYGSNGP LGGWLDEHNL QIMFSWPGMV LVTIFVTCPF VVRELVPVML SQGSQEDEAA
ILLGASGWQM FRRVTLPNIR WALLYGVVLT NARAIGEFGA VSVVSGSIRG ETLSLPLQIE
LLEQDYNTVG SFTAAALLTL MAIITLFLKS MLQWRLENQE KRAQQEEHHE H
