ID   CYSZ_CUCMA              Reviewed;         516 AA.
AC   P49299;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Citrate synthase, glyoxysomal;
DE            EC=2.3.3.16;
DE   AltName: Full=GCS;
DE   Flags: Precursor;
OS   Cucurbita maxima (Pumpkin) (Winter squash).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Cucurbitales; Cucurbitaceae; Cucurbiteae; Cucurbita.
OX   NCBI_TaxID=3661;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 44-51.
RC   STRAIN=cv. Kurokawa Amakuri Nankin; TISSUE=Etiolated cotyledon;
RX   PubMed=7888626; DOI=10.1007/bf00020191;
RA   Kato A., Hayashi M., Mori H., Nishimura M.;
RT   "Molecular characterization of a glyoxysomal citrate synthase that is
RT   synthesized as a precursor of higher molecular mass in pumpkin.";
RL   Plant Mol. Biol. 27:377-390(1995).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC         Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.16; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU10117};
CC   -!- PATHWAY: Carbohydrate metabolism; glyoxylate cycle; isocitrate from
CC       oxaloacetate: step 1/2.
CC   -!- SUBCELLULAR LOCATION: Glyoxysome.
CC   -!- MISCELLANEOUS: Citrate synthase is found in nearly all cells capable of
CC       oxidative metabolism.
CC   -!- SIMILARITY: Belongs to the citrate synthase family. {ECO:0000305}.
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DR   EMBL; D38132; BAA07328.1; -; mRNA.
DR   PIR; S53008; S53008.
DR   AlphaFoldDB; P49299; -.
DR   SMR; P49299; -.
DR   PRIDE; P49299; -.
DR   OrthoDB; 691172at2759; -.
DR   UniPathway; UPA00703; UER00717.
DR   Proteomes; UP000504608; Unplaced.
DR   GO; GO:0009514; C:glyoxysome; IEA:UniProtKB-SubCell.
DR   GO; GO:0036440; F:citrate synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.230.10; -; 1.
DR   Gene3D; 1.10.580.10; -; 1.
DR   InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR   InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR   InterPro; IPR002020; Citrate_synthase.
DR   InterPro; IPR019810; Citrate_synthase_AS.
DR   InterPro; IPR036969; Citrate_synthase_sf.
DR   PANTHER; PTHR11739; PTHR11739; 1.
DR   Pfam; PF00285; Citrate_synt; 1.
DR   PRINTS; PR00143; CITRTSNTHASE.
DR   SUPFAM; SSF48256; SSF48256; 1.
DR   PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Glyoxylate bypass; Glyoxysome; Peroxisome;
KW   Reference proteome; Transferase; Transit peptide; Tricarboxylic acid cycle.
FT   TRANSIT         1..43
FT                   /note="Glyoxysome"
FT                   /evidence="ECO:0000269|PubMed:7888626"
FT   CHAIN           44..516
FT                   /note="Citrate synthase, glyoxysomal"
FT                   /id="PRO_0000005490"
FT   ACT_SITE        329
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   ACT_SITE        368
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   ACT_SITE        424
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
SQ   SEQUENCE   516 AA;  56759 MW;  162F6270284A3F6B CRC64;
     MPTDMELSPS NVARHRLAVL AAHLSAASLE PPVMASSLEA HCVSAQTMVA PPELVKGTLT
     IVDERTGKRY QVQVSEEGTI KATDLKKITT GPNDKGLKLY DPGYLNTAPV RSSISYIDGD
     LGILRYRGYP IEELAESSTY VEVAYLLMYG NLPSQSQLAD WEFAISQHSA VPQGLVDIIQ
     AMPHDAHPMG VLVSAMSALS VFHPDANPAL RGQDLYKSKQ VRDKQIARII GKAPTIAAAA
     YLRLAGRPPV LPSSNLSYSE NFLYMLDSLG NRSYKPNPRL ARVLDILFIL HAEHEMNCST
     SAARHLASSG VDVFTALSGA VGALYGPLHG GANEAVLKML SEIGTVNNIP EFIEGVKNRK
     RKMSGFGHRV YKNYDPRAKV IRKLAEEVFS IVGRDPLIEV AVALEKAALS DEYFVKRKLY
     PNVDFYSGLI YRAMGFPPEF FTVLFAIPRM AGYLAHWRES LDDPDTKIIR PQQVYTGEWL
     RHYIPPNERL VPAKADRLGQ VSVSNASKRR LSGSGI