ID   CYSZ_PSEA8              Reviewed;         246 AA.
AC   B7UY90;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 1.
DT   25-MAY-2022, entry version 64.
DE   RecName: Full=Sulfate transporter CysZ {ECO:0000255|HAMAP-Rule:MF_00468};
GN   Name=cysZ {ECO:0000255|HAMAP-Rule:MF_00468}; OrderedLocusNames=PLES_44721;
OS   Pseudomonas aeruginosa (strain LESB58).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=557722;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LESB58;
RX   PubMed=19047519; DOI=10.1101/gr.086082.108;
RA   Winstanley C., Langille M.G.I., Fothergill J.L., Kukavica-Ibrulj I.,
RA   Paradis-Bleau C., Sanschagrin F., Thomson N.R., Winsor G.L., Quail M.A.,
RA   Lennard N., Bignell A., Clarke L., Seeger K., Saunders D., Harris D.,
RA   Parkhill J., Hancock R.E.W., Brinkman F.S.L., Levesque R.C.;
RT   "Newly introduced genomic prophage islands are critical determinants of in
RT   vivo competitiveness in the Liverpool epidemic strain of Pseudomonas
RT   aeruginosa.";
RL   Genome Res. 19:12-23(2009).
CC   -!- FUNCTION: High affinity, high specificity proton-dependent sulfate
CC       transporter, which mediates sulfate uptake. Provides the sulfur source
CC       for the cysteine synthesis pathway. {ECO:0000255|HAMAP-Rule:MF_00468}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_00468}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_00468}.
CC   -!- SIMILARITY: Belongs to the CysZ family. {ECO:0000255|HAMAP-
CC       Rule:MF_00468}.
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DR   EMBL; FM209186; CAW29227.1; -; Genomic_DNA.
DR   RefSeq; WP_003116482.1; NC_011770.1.
DR   AlphaFoldDB; B7UY90; -.
DR   SMR; B7UY90; -.
DR   KEGG; pag:PLES_44721; -.
DR   HOGENOM; CLU_070331_1_0_6; -.
DR   OMA; PFADDWS; -.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0015116; F:sulfate transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019344; P:cysteine biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0000103; P:sulfate assimilation; IEA:InterPro.
DR   HAMAP; MF_00468; CysZ; 1.
DR   InterPro; IPR022985; Sulfate_CysZ.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Cell inner membrane; Cell membrane;
KW   Cysteine biosynthesis; Membrane; Sulfate transport; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..246
FT                   /note="Sulfate transporter CysZ"
FT                   /id="PRO_1000125501"
FT   TRANSMEM        24..44
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00468"
FT   TRANSMEM        69..89
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00468"
FT   TRANSMEM        148..168
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00468"
FT   TRANSMEM        214..234
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00468"
SQ   SEQUENCE   246 AA;  27936 MW;  1FA7ABE3D893FE6E CRC64;
     MPALSGPQYL GEGLKLIMRP GLRLFVLIPL TLNLLVFALL IGFAMQQFSH WVDLLMPSLP
     DWLSFLQYIV WPLFVLLVLV IVFFTFTMVA NIISAPFNGF LSEKVEVVVR GRDDFPPFSW
     AELLAMIPRT MGREMRKLAY FLPRALVLLV LSFVPGVNLI ATPLWILFGI WMMAVQYIDY
     PADNHKLGWN EMLAWLRSKR WACMGFGGVT YLALLIPLVN LVMMPAAVAG ATLFWVREEG
     EKALVK