ID   CYSZ_PSEAE              Reviewed;         246 AA.
AC   Q9I595;
DT   02-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 108.
DE   RecName: Full=Sulfate transporter CysZ {ECO:0000255|HAMAP-Rule:MF_00468};
GN   Name=cysZ {ECO:0000255|HAMAP-Rule:MF_00468}; OrderedLocusNames=PA0846;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- FUNCTION: High affinity, high specificity proton-dependent sulfate
CC       transporter, which mediates sulfate uptake. Provides the sulfur source
CC       for the cysteine synthesis pathway. {ECO:0000255|HAMAP-Rule:MF_00468}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_00468}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_00468}.
CC   -!- SIMILARITY: Belongs to the CysZ family. {ECO:0000255|HAMAP-
CC       Rule:MF_00468}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE004091; AAG04235.1; -; Genomic_DNA.
DR   PIR; D83540; D83540.
DR   RefSeq; NP_249537.1; NC_002516.2.
DR   RefSeq; WP_003115194.1; NZ_QZGE01000007.1.
DR   AlphaFoldDB; Q9I595; -.
DR   SMR; Q9I595; -.
DR   STRING; 287.DR97_1098; -.
DR   PaxDb; Q9I595; -.
DR   DNASU; 880298; -.
DR   EnsemblBacteria; AAG04235; AAG04235; PA0846.
DR   GeneID; 880298; -.
DR   KEGG; pae:PA0846; -.
DR   PATRIC; fig|208964.12.peg.878; -.
DR   PseudoCAP; PA0846; -.
DR   HOGENOM; CLU_070331_1_0_6; -.
DR   InParanoid; Q9I595; -.
DR   OMA; PFADDWS; -.
DR   PhylomeDB; Q9I595; -.
DR   BioCyc; PAER208964:G1FZ6-861-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR   GO; GO:0009675; F:high-affinity sulfate:proton symporter activity; IBA:GO_Central.
DR   GO; GO:0019344; P:cysteine biosynthetic process; IBA:GO_Central.
DR   GO; GO:0000103; P:sulfate assimilation; IBA:GO_Central.
DR   HAMAP; MF_00468; CysZ; 1.
DR   InterPro; IPR022985; Sulfate_CysZ.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Cell inner membrane; Cell membrane;
KW   Cysteine biosynthesis; Membrane; Reference proteome; Sulfate transport;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..246
FT                   /note="Sulfate transporter CysZ"
FT                   /id="PRO_0000204344"
FT   TRANSMEM        24..44
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00468"
FT   TRANSMEM        69..89
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00468"
FT   TRANSMEM        148..168
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00468"
FT   TRANSMEM        214..234
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00468"
SQ   SEQUENCE   246 AA;  27964 MW;  1FA7ABE3CFA3FE6E CRC64;
     MPALSGPQYL GEGLKLIMRP GLRLFVLIPL TLNLLVFALL IGFAMQQFSH WVDLLMPSLP
     DWLSFLQYIV WPLFVLLVLV IVFFTFTMVA NIISAPFNGF LSEKVEVVVR GRDDFPPFSW
     AELLAMIPRT MGREMRKLAY FLPRALVLLV LSFVPGVNLI ATPLWILFGI WMMAVQYIDY
     PADNHKLGWN EMLAWLRSKR WACMGFGGVT YLALLIPLVN LVMMPAAVAG ATLFWVREEG
     ERALVK