ID   CYSZ_PSEF5              Reviewed;         249 AA.
AC   Q4KI58;
DT   13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 2.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=Sulfate transporter CysZ {ECO:0000255|HAMAP-Rule:MF_00468};
GN   Name=cysZ {ECO:0000255|HAMAP-Rule:MF_00468}; OrderedLocusNames=PFL_0944;
OS   Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=220664;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-477 / NRRL B-23932 / Pf-5;
RX   PubMed=15980861; DOI=10.1038/nbt1110;
RA   Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A.,
RA   Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J.,
RA   Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J.,
RA   Gwinn M.L., Zhou L., Schneider D.J., Cartinhour S.W., Nelson W.C.,
RA   Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III,
RA   Thomashow L.S., Loper J.E.;
RT   "Complete genome sequence of the plant commensal Pseudomonas fluorescens
RT   Pf-5.";
RL   Nat. Biotechnol. 23:873-878(2005).
CC   -!- FUNCTION: High affinity, high specificity proton-dependent sulfate
CC       transporter, which mediates sulfate uptake. Provides the sulfur source
CC       for the cysteine synthesis pathway. {ECO:0000255|HAMAP-Rule:MF_00468}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_00468}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_00468}.
CC   -!- SIMILARITY: Belongs to the CysZ family. {ECO:0000255|HAMAP-
CC       Rule:MF_00468}.
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DR   EMBL; CP000076; AAY90231.2; -; Genomic_DNA.
DR   RefSeq; WP_011059298.1; NC_004129.6.
DR   AlphaFoldDB; Q4KI58; -.
DR   SMR; Q4KI58; -.
DR   STRING; 220664.PFL_0944; -.
DR   DNASU; 3475802; -.
DR   EnsemblBacteria; AAY90231; AAY90231; PFL_0944.
DR   KEGG; pfl:PFL_0944; -.
DR   PATRIC; fig|220664.5.peg.968; -.
DR   eggNOG; COG2981; Bacteria.
DR   HOGENOM; CLU_070331_1_0_6; -.
DR   OMA; PFADDWS; -.
DR   OrthoDB; 1972181at2; -.
DR   Proteomes; UP000008540; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0015116; F:sulfate transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019344; P:cysteine biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0000103; P:sulfate assimilation; IEA:InterPro.
DR   HAMAP; MF_00468; CysZ; 1.
DR   InterPro; IPR022985; Sulfate_CysZ.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Cell inner membrane; Cell membrane;
KW   Cysteine biosynthesis; Membrane; Reference proteome; Sulfate transport;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..249
FT                   /note="Sulfate transporter CysZ"
FT                   /id="PRO_0000204345"
FT   TRANSMEM        26..46
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00468"
FT   TRANSMEM        71..91
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00468"
FT   TRANSMEM        150..170
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00468"
FT   TRANSMEM        206..226
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00468"
SQ   SEQUENCE   249 AA;  28147 MW;  BA2AF8171F223AA1 CRC64;
     MPAPVLSGPQ YLREGLKLVL SPGLRLFVLL PLAINLVLFV GLIYFAGHQF SLWVDALMPS
     LPSWLSFLSY VIWPLFVVLV AFMVFFSFTM LANIIAAPFN GFLSEKVETV VRGTDDFPPF
     SWGELIAMIP RTLAREMRKL GYFLPRAIGL FILSLIPVVN LIAAPLWLLF GIWMMAIQYI
     DYPADNHKMS WQDMLAWLRQ KRWQSLGFGG IVYLALLIPL VNILMMPAAV AGATLFWVRE
     RGVENLPAK