ID   CYSZ_PSEPF              Reviewed;         250 AA.
AC   Q3KHX7;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   08-NOV-2005, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=Sulfate transporter CysZ {ECO:0000255|HAMAP-Rule:MF_00468};
GN   Name=cysZ {ECO:0000255|HAMAP-Rule:MF_00468}; OrderedLocusNames=Pfl01_0886;
OS   Pseudomonas fluorescens (strain Pf0-1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=205922;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Pf0-1;
RX   PubMed=19432983; DOI=10.1186/gb-2009-10-5-r51;
RA   Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R.,
RA   Jackson R.W., Preston G.M., Zhang X.-X., Moon C.D., Gehrig S.M.,
RA   Godfrey S.A.C., Knight C.G., Malone J.G., Robinson Z., Spiers A.J.,
RA   Harris S., Challis G.L., Yaxley A.M., Harris D., Seeger K., Murphy L.,
RA   Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K.,
RA   Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M.,
RA   Parkhill J., Levy S.B., Rainey P.B., Thomson N.R.;
RT   "Genomic and genetic analyses of diversity and plant interactions of
RT   Pseudomonas fluorescens.";
RL   Genome Biol. 10:R51.1-R51.16(2009).
CC   -!- FUNCTION: High affinity, high specificity proton-dependent sulfate
CC       transporter, which mediates sulfate uptake. Provides the sulfur source
CC       for the cysteine synthesis pathway. {ECO:0000255|HAMAP-Rule:MF_00468}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_00468}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_00468}.
CC   -!- SIMILARITY: Belongs to the CysZ family. {ECO:0000255|HAMAP-
CC       Rule:MF_00468}.
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DR   EMBL; CP000094; ABA72629.1; -; Genomic_DNA.
DR   RefSeq; WP_011332493.1; NC_007492.2.
DR   AlphaFoldDB; Q3KHX7; -.
DR   SMR; Q3KHX7; -.
DR   STRING; 205922.Pfl01_0886; -.
DR   EnsemblBacteria; ABA72629; ABA72629; Pfl01_0886.
DR   KEGG; pfo:Pfl01_0886; -.
DR   eggNOG; COG2981; Bacteria.
DR   HOGENOM; CLU_070331_1_0_6; -.
DR   OMA; PFADDWS; -.
DR   Proteomes; UP000002704; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0015116; F:sulfate transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019344; P:cysteine biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0000103; P:sulfate assimilation; IEA:InterPro.
DR   HAMAP; MF_00468; CysZ; 1.
DR   InterPro; IPR022985; Sulfate_CysZ.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Cell inner membrane; Cell membrane;
KW   Cysteine biosynthesis; Membrane; Sulfate transport; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..250
FT                   /note="Sulfate transporter CysZ"
FT                   /id="PRO_1000013716"
FT   TRANSMEM        26..46
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00468"
FT   TRANSMEM        71..91
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00468"
FT   TRANSMEM        150..170
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00468"
FT   TRANSMEM        211..231
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00468"
SQ   SEQUENCE   250 AA;  28301 MW;  D19F0ADF2DFF42F2 CRC64;
     MPAPVLSGPQ YLREGLKLVL SPGLRLFVLL PLAINLVLFV GLIYLAGHQF SLWVDTLMPS
     LPEWLSFLSY ILWPLFVVLV ALMVFFTFTM LANVIAAPFN GFLAEKVEVV VRGTDDFPAF
     SWGELIAMIP RTLAREMRKL GYFLPRAIGL FILSFIPVVN IVAAPLWLLF GVWMMAIQYI
     DYPADNHKLG WNEMLAWLRQ KRWQSMSFGG IVYLVLLIPV VNILMMPAAV AGATLFWVRE
     RGAENLVTQR