ID   CYSZ_PSESM              Reviewed;         253 AA.
AC   Q887V6;
DT   11-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 97.
DE   RecName: Full=Sulfate transporter CysZ {ECO:0000255|HAMAP-Rule:MF_00468};
GN   Name=cysZ {ECO:0000255|HAMAP-Rule:MF_00468}; OrderedLocusNames=PSPTO_1177;
OS   Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=223283;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-871 / DC3000;
RX   PubMed=12928499; DOI=10.1073/pnas.1731982100;
RA   Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T.,
RA   Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA   Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C.,
RA   Davidsen T.M., Zafar N., Zhou L., Liu J., Yuan Q., Khouri H.M.,
RA   Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R.,
RA   Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R.,
RA   Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G.,
RA   Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M.,
RA   Collmer A.;
RT   "The complete genome sequence of the Arabidopsis and tomato pathogen
RT   Pseudomonas syringae pv. tomato DC3000.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003).
CC   -!- FUNCTION: High affinity, high specificity proton-dependent sulfate
CC       transporter, which mediates sulfate uptake. Provides the sulfur source
CC       for the cysteine synthesis pathway. {ECO:0000255|HAMAP-Rule:MF_00468}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_00468}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_00468}.
CC   -!- SIMILARITY: Belongs to the CysZ family. {ECO:0000255|HAMAP-
CC       Rule:MF_00468}.
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DR   EMBL; AE016853; AAO54704.1; -; Genomic_DNA.
DR   RefSeq; NP_791009.1; NC_004578.1.
DR   RefSeq; WP_007244082.1; NC_004578.1.
DR   AlphaFoldDB; Q887V6; -.
DR   SMR; Q887V6; -.
DR   STRING; 223283.PSPTO_1177; -.
DR   EnsemblBacteria; AAO54704; AAO54704; PSPTO_1177.
DR   GeneID; 1182813; -.
DR   KEGG; pst:PSPTO_1177; -.
DR   PATRIC; fig|223283.9.peg.1193; -.
DR   eggNOG; COG2981; Bacteria.
DR   HOGENOM; CLU_070331_1_0_6; -.
DR   OMA; PFADDWS; -.
DR   OrthoDB; 1972181at2; -.
DR   PhylomeDB; Q887V6; -.
DR   Proteomes; UP000002515; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0015116; F:sulfate transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019344; P:cysteine biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0000103; P:sulfate assimilation; IEA:InterPro.
DR   HAMAP; MF_00468; CysZ; 1.
DR   InterPro; IPR022985; Sulfate_CysZ.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Cell inner membrane; Cell membrane;
KW   Cysteine biosynthesis; Membrane; Reference proteome; Sulfate transport;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..253
FT                   /note="Sulfate transporter CysZ"
FT                   /id="PRO_0000204346"
FT   TRANSMEM        27..47
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00468"
FT   TRANSMEM        71..91
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00468"
FT   TRANSMEM        150..170
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00468"
FT   TRANSMEM        211..231
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00468"
SQ   SEQUENCE   253 AA;  28487 MW;  C80D33A4305C3146 CRC64;
     MPAPALTGPQ YLREGLKLIL SPGLRLFVLL PLSINIVLFC GLIYLAVHQF ELWVDTFMPT
     LPHWLSFLSY ILWPLFVALV LLMVFFTFTV VANIIAAPFN GFLSEKVEAV IRGVDESPDF
     SWAELVAMVP RTLAREARKL GYMLPRMLGL FILSFIPVAN IIAAPLWLLF GVWMMAIQYI
     DYPADNHKLG WNEMLGWLKS KRWQSLSFGG IVYVALLIPV VNLLMMPAAV AAATLFWVRE
     RGADALPIAH ARD