ID   CYSZ_VIBCH              Reviewed;         250 AA.
AC   Q9KTD3;
DT   02-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=Sulfate transporter CysZ {ECO:0000255|HAMAP-Rule:MF_00468};
GN   Name=cysZ {ECO:0000255|HAMAP-Rule:MF_00468}; OrderedLocusNames=VC_0969;
OS   Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=243277;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX   PubMed=10952301; DOI=10.1038/35020000;
RA   Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA   Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA   Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA   Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA   Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA   Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT   "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT   cholerae.";
RL   Nature 406:477-483(2000).
CC   -!- FUNCTION: High affinity, high specificity proton-dependent sulfate
CC       transporter, which mediates sulfate uptake. Provides the sulfur source
CC       for the cysteine synthesis pathway. {ECO:0000255|HAMAP-Rule:MF_00468}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_00468}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_00468}.
CC   -!- SIMILARITY: Belongs to the CysZ family. {ECO:0000255|HAMAP-
CC       Rule:MF_00468}.
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DR   EMBL; AE003852; AAF94131.1; -; Genomic_DNA.
DR   PIR; D82258; D82258.
DR   RefSeq; NP_230616.1; NC_002505.1.
DR   RefSeq; WP_001167408.1; NZ_LT906614.1.
DR   AlphaFoldDB; Q9KTD3; -.
DR   SMR; Q9KTD3; -.
DR   STRING; 243277.VC_0969; -.
DR   DNASU; 2614222; -.
DR   EnsemblBacteria; AAF94131; AAF94131; VC_0969.
DR   KEGG; vch:VC_0969; -.
DR   PATRIC; fig|243277.26.peg.922; -.
DR   eggNOG; COG2981; Bacteria.
DR   HOGENOM; CLU_070331_1_0_6; -.
DR   OMA; PFADDWS; -.
DR   BioCyc; VCHO:VC0969-MON; -.
DR   Proteomes; UP000000584; Chromosome 1.
DR   GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR   GO; GO:0009675; F:high-affinity sulfate:proton symporter activity; IBA:GO_Central.
DR   GO; GO:0019344; P:cysteine biosynthetic process; IBA:GO_Central.
DR   GO; GO:0000103; P:sulfate assimilation; IBA:GO_Central.
DR   HAMAP; MF_00468; CysZ; 1.
DR   InterPro; IPR022985; Sulfate_CysZ.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Cell inner membrane; Cell membrane;
KW   Cysteine biosynthesis; Membrane; Reference proteome; Sulfate transport;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..250
FT                   /note="Sulfate transporter CysZ"
FT                   /id="PRO_0000204351"
FT   TRANSMEM        27..47
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00468"
FT   TRANSMEM        64..84
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00468"
FT   TRANSMEM        150..170
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00468"
FT   TRANSMEM        210..230
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00468"
SQ   SEQUENCE   250 AA;  28439 MW;  2EE431ED1A3780F0 CRC64;
     MQISSRSGFG YFSYGIRLAL TPGIRRFVVL PLLANIILVG GAMYYLFSHL DTWISEWIGQ
     LPSFLSWLSY VLWPLLALTI LATFSYFFST LANFIASPFN GLLAEKVEQH LSGERIGEEG
     VWALVKDVPR ILSREWRKLL YVLPKALGLF LLLLIPALGQ TLGPIAWFLF TAWMLAIQYC
     DYPFDNHKIS FHDMRNTLKQ NQSKAYGFGM LVAFFTSIPI VNLFIVPVAV CGATAMWVME
     FKIQHSPLRQ