ID   CYT12_ORYSJ             Reviewed;         250 AA.
AC   Q0JNR2; B7F4W8; Q5NBI1;
DT   06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2006, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Cysteine proteinase inhibitor 12;
DE   AltName: Full=Oryzacystatin XII;
DE            Short=OC-XII;
DE   AltName: Full=Oryzacystatin-12;
DE   Flags: Precursor;
GN   OrderedLocusNames=Os01g0270100, LOC_Os01g16430; ORFNames=P0667A10.20;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12447438; DOI=10.1038/nature01184;
RA   Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA   Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA   Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA   Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA   Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA   Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA   Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA   Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA   Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA   Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA   Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA   Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA   Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT   "The genome sequence and structure of rice chromosome 1.";
RL   Nature 420:312-316(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [6]
RP   GENE FAMILY.
RX   PubMed=15887031; DOI=10.1007/s00438-005-1147-4;
RA   Martinez M., Abraham Z., Carbonero P., Diaz I.;
RT   "Comparative phylogenetic analysis of cystatin gene families from
RT   arabidopsis, rice and barley.";
RL   Mol. Genet. Genomics 273:423-432(2005).
CC   -!- FUNCTION: Specific inhibitor of cysteine proteinases. Probably involved
CC       in the regulation of endogenous processes and in defense against pests
CC       and pathogens (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the cystatin family. Phytocystatin subfamily.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAD81175.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AP001073; BAD81175.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AP008207; BAF04616.1; -; Genomic_DNA.
DR   EMBL; AP014957; BAS71500.1; -; Genomic_DNA.
DR   EMBL; AK119511; BAG99665.1; -; mRNA.
DR   RefSeq; XP_015621945.1; XM_015766459.1.
DR   AlphaFoldDB; Q0JNR2; -.
DR   SMR; Q0JNR2; -.
DR   STRING; 4530.OS01T0270100-01; -.
DR   MEROPS; I25.014; -.
DR   PaxDb; Q0JNR2; -.
DR   EnsemblPlants; Os01t0270100-01; Os01t0270100-01; Os01g0270100.
DR   GeneID; 4326272; -.
DR   Gramene; Os01t0270100-01; Os01t0270100-01; Os01g0270100.
DR   KEGG; osa:4326272; -.
DR   eggNOG; ENOG502QRXR; Eukaryota.
DR   HOGENOM; CLU_072701_1_0_1; -.
DR   InParanoid; Q0JNR2; -.
DR   OMA; HNNEGAF; -.
DR   OrthoDB; 1565344at2759; -.
DR   Proteomes; UP000000763; Chromosome 1.
DR   Proteomes; UP000059680; Chromosome 1.
DR   ExpressionAtlas; Q0JNR2; baseline and differential.
DR   Genevisible; Q0JNR2; OS.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IBA:GO_Central.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   CDD; cd00042; CY; 2.
DR   InterPro; IPR027214; Cystatin.
DR   InterPro; IPR000010; Cystatin_dom.
DR   InterPro; IPR046350; Cystatin_sf.
DR   InterPro; IPR018073; Prot_inh_cystat_CS.
DR   PANTHER; PTHR11413; PTHR11413; 1.
DR   Pfam; PF16845; SQAPI; 1.
DR   SMART; SM00043; CY; 1.
DR   SUPFAM; SSF54403; SSF54403; 2.
DR   PROSITE; PS00287; CYSTATIN; 1.
PE   2: Evidence at transcript level;
KW   Plant defense; Protease inhibitor; Reference proteome; Repeat; Secreted;
KW   Signal; Thiol protease inhibitor.
FT   SIGNAL          1..32
FT                   /evidence="ECO:0000255"
FT   CHAIN           33..250
FT                   /note="Cysteine proteinase inhibitor 12"
FT                   /id="PRO_0000277509"
FT   DOMAIN          49..137
FT                   /note="Cystatin 1"
FT   DOMAIN          156..202
FT                   /note="Cystatin 2"
FT   MOTIF           93..97
FT                   /note="Secondary area of contact"
FT                   /evidence="ECO:0000250"
FT   SITE            49
FT                   /note="Reactive site"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   250 AA;  27269 MW;  6F2A7D4E8B8AF66F CRC64;
     MRVAATTRPA SSSAAAPLPL FLLLAVAAAA AALFLVGSAS LAMAGHVLGG AHDAPSAANS
     VETDALARFA VDEHNKRENA LLEFVRVVEA KEQVVAGTLH HLTLEALEAG RKKVYEAKVW
     VKPWLDFKEL QEFRNTGDAT TFTNADLGAK KGGHEPGWRD VPVHDPVVKD AADHAVKSIQ
     QRSNSLFPYE LLEIVRAKAE VVEDFAKFDI LMKLKRGNKE EKFKAEVHKN LEGAFVLNQM
     QQEHDESSSQ