CYT1_ACTCC
ID CYT1_ACTCC Reviewed; 116 AA.
AC P86472; A0A2R6Q9U2;
DT 20-APR-2010, integrated into UniProtKB/Swiss-Prot.
DT 13-NOV-2019, sequence version 2.
DT 25-MAY-2022, entry version 16.
DE RecName: Full=Cysteine proteinase inhibitor 1 {ECO:0000303|PubMed:14697268};
DE Short=KCPI1 {ECO:0000303|PubMed:14697268};
DE AltName: Full=Phytocystatin {ECO:0000250|UniProtKB:Q6TPK4};
DE Flags: Precursor;
GN Name=CYT1 {ECO:0000305};
GN ORFNames=CEY00_Acc20523 {ECO:0000312|EMBL:PSS04667.1};
OS Actinidia chinensis var. chinensis (Chinese soft-hair kiwi).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; Ericales; Actinidiaceae; Actinidia.
OX NCBI_TaxID=1590841;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Red5;
RX PubMed=29661190; DOI=10.1186/s12864-018-4656-3;
RA Pilkington S.M., Crowhurst R., Hilario E., Nardozza S., Fraser L., Peng Y.,
RA Gunaseelan K., Simpson R., Tahir J., Deroles S.C., Templeton K., Luo Z.,
RA Davy M., Cheng C., McNeilage M., Scaglione D., Liu Y., Zhang Q., Datson P.,
RA De Silva N., Gardiner S.E., Bassett H., Chagne D., McCallum J.,
RA Dzierzon H., Deng C., Wang Y.Y., Barron L., Manako K., Bowen J.,
RA Foster T.M., Erridge Z.A., Tiffin H., Waite C.N., Davies K.M.,
RA Grierson E.P., Laing W.A., Kirk R., Chen X., Wood M., Montefiori M.,
RA Brummell D.A., Schwinn K.E., Catanach A., Fullerton C., Li D.,
RA Meiyalaghan S., Nieuwenhuizen N., Read N., Prakash R., Hunter D., Zhang H.,
RA McKenzie M., Knabel M., Harris A., Allan A.C., Gleave A., Chen A.,
RA Janssen B.J., Plunkett B., Ampomah-Dwamena C., Voogd C., Leif D.,
RA Lafferty D., Souleyre E.J.F., Varkonyi-Gasic E., Gambi F., Hanley J.,
RA Yao J.L., Cheung J., David K.M., Warren B., Marsh K., Snowden K.C.,
RA Lin-Wang K., Brian L., Martinez-Sanchez M., Wang M., Ileperuma N.,
RA Macnee N., Campin R., McAtee P., Drummond R.S.M., Espley R.V.,
RA Ireland H.S., Wu R., Atkinson R.G., Karunairetnam S., Bulley S.,
RA Chunkath S., Hanley Z., Storey R., Thrimawithana A.H., Thomson S.,
RA David C., Testolin R., Huang H., Hellens R.P., Schaffer R.J.;
RT "A manually annotated Actinidia chinensis var. chinensis (kiwifruit) genome
RT highlights the challenges associated with draft genomes and gene prediction
RT in plants.";
RL BMC Genomics 19:257-257(2018).
RN [2]
RP PROTEIN SEQUENCE OF 27-46.
RC STRAIN=cv. Hort 16A {ECO:0000269|PubMed:14697268};
RC TISSUE=Seed {ECO:0000269|PubMed:14697268};
RX PubMed=14697268; DOI=10.1016/j.phytochem.2003.09.019;
RA Rassam M., Laing W.A.;
RT "Purification and characterization of phytocystatins from kiwifruit cortex
RT and seeds.";
RL Phytochemistry 65:19-30(2004).
RN [3]
RP PROTEIN SEQUENCE OF 27-37, AND ALLERGEN.
RC STRAIN=cv. Hort 16A {ECO:0000269|PubMed:15536427};
RC TISSUE=Fruit {ECO:0000269|PubMed:15536427};
RX PubMed=15536427; DOI=10.1016/j.jaci.2004.07.016;
RA Bublin M., Mari A., Ebner C., Knulst A., Scheiner O.,
RA Hoffmann-Sommergruber K., Breiteneder H., Radauer C.;
RT "IgE sensitization profiles toward green and gold kiwifruits differ among
RT patients allergic to kiwifruit from 3 European countries.";
RL J. Allergy Clin. Immunol. 114:1169-1175(2004).
CC -!- FUNCTION: Specific inhibitor of papain family cysteine proteinases.
CC {ECO:0000250|UniProtKB:Q6TPK4}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P01035}.
CC -!- ALLERGEN: Causes an allergic reaction in human. Binds IgE.
CC {ECO:0000269|PubMed:15536427}.
CC -!- SIMILARITY: Belongs to the cystatin family. Phytocystatin subfamily.
CC {ECO:0000305}.
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DR EMBL; NKQK01000018; PSS04667.1; -; Genomic_DNA.
DR AlphaFoldDB; P86472; -.
DR SMR; P86472; -.
DR Allergome; 2406; Act c 4.
DR EnsemblPlants; PSS04667; PSS04667; CEY00_Acc20523.
DR Gramene; PSS04667; PSS04667; CEY00_Acc20523.
DR OMA; SFEPANN; -.
DR Proteomes; UP000241394; Chromosome lg18.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR CDD; cd00042; CY; 1.
DR InterPro; IPR000010; Cystatin_dom.
DR InterPro; IPR046350; Cystatin_sf.
DR Pfam; PF16845; SQAPI; 1.
DR SMART; SM00043; CY; 1.
DR SUPFAM; SSF54403; SSF54403; 1.
PE 1: Evidence at protein level;
KW Allergen; Direct protein sequencing; Glycoprotein; Plant defense;
KW Protease inhibitor; Reference proteome; Secreted; Signal;
KW Thiol protease inhibitor.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..116
FT /note="Cysteine proteinase inhibitor 1"
FT /id="PRO_0000393865"
FT DOMAIN 30..89
FT /note="Cystatin"
FT /evidence="ECO:0000255"
FT MOTIF 73..77
FT /note="Secondary area of contact"
FT /evidence="ECO:0000250|UniProtKB:P01035"
FT SITE 30
FT /note="Reactive site"
FT /evidence="ECO:0000250|UniProtKB:P01035"
FT CARBOHYD 109
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CONFLICT 37
FT /note="N -> E (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 37
FT /note="N -> S (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 116 AA; 12792 MW; 2E58EFF45446C1BE CRC64;
MVPKPLSLLL LLLLALSAAV VGGRKRVAAG GWRPIENLNS AEVQDVAQFA VSEHNKQAND
ELQYQSVVRG YTQVVAGTNY RLVIAAKDGA VVGNYEAVVW DKPWMHFRNL TSFRKV
