ID   CYT1_MAIZE              Reviewed;         135 AA.
AC   P31726;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=Cystatin-1;
DE   AltName: Full=Corn kernel cysteine proteinase inhibitor;
DE   AltName: Full=Cystatin I;
DE   Flags: Precursor;
GN   Name=RAMDAZC7;
OS   Zea mays (Maize).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX   NCBI_TaxID=4577;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Koshu; TISSUE=Immature kernel;
RX   PubMed=1425699; DOI=10.1111/j.1432-1033.1992.tb17365.x;
RA   Abe M., Abe K., Kuroda M., Arai S.;
RT   "Corn kernel cysteine proteinase inhibitor as a novel cystatin superfamily
RT   member of plant origin. Molecular cloning and expression studies.";
RL   Eur. J. Biochem. 209:933-937(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 84-135.
RC   STRAIN=cv. B73;
RX   PubMed=8278499; DOI=10.1104/pp.101.1.329;
RA   Keith C.S., Hoang D.O., Barrett B.M., Feigelman B., Nelson M.C., Thai H.,
RA   Baysdorfer C.;
RT   "Partial sequence analysis of 130 randomly selected maize cDNA clones.";
RL   Plant Physiol. 101:329-332(1993).
CC   -!- DEVELOPMENTAL STAGE: Reaches a maximum 2 weeks after flowering and then
CC       decreases gradually.
CC   -!- SIMILARITY: Belongs to the cystatin family. Phytocystatin subfamily.
CC       {ECO:0000305}.
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DR   EMBL; D10622; BAA01472.1; -; mRNA.
DR   EMBL; M95070; AAA18557.1; -; mRNA.
DR   PIR; S27239; S27239.
DR   RefSeq; NP_001105295.1; NM_001111825.1.
DR   AlphaFoldDB; P31726; -.
DR   SMR; P31726; -.
DR   STRING; 4577.GRMZM2G438551_P01; -.
DR   MEROPS; I25.052; -.
DR   PaxDb; P31726; -.
DR   PRIDE; P31726; -.
DR   GeneID; 542213; -.
DR   KEGG; zma:542213; -.
DR   MaizeGDB; 25463; -.
DR   MaizeGDB; 30117; -.
DR   eggNOG; ENOG502SC50; Eukaryota.
DR   OrthoDB; 1565344at2759; -.
DR   Proteomes; UP000007305; Unplaced.
DR   ExpressionAtlas; P31726; baseline and differential.
DR   GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IBA:GO_Central.
DR   CDD; cd00042; CY; 1.
DR   InterPro; IPR027214; Cystatin.
DR   InterPro; IPR000010; Cystatin_dom.
DR   InterPro; IPR046350; Cystatin_sf.
DR   InterPro; IPR018073; Prot_inh_cystat_CS.
DR   PANTHER; PTHR11413; PTHR11413; 1.
DR   Pfam; PF16845; SQAPI; 1.
DR   SMART; SM00043; CY; 1.
DR   SUPFAM; SSF54403; SSF54403; 1.
DR   PROSITE; PS00287; CYSTATIN; 1.
PE   2: Evidence at transcript level;
KW   Protease inhibitor; Reference proteome; Signal; Thiol protease inhibitor.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..135
FT                   /note="Cystatin-1"
FT                   /id="PRO_0000006668"
FT   MOTIF           86..90
FT                   /note="Secondary area of contact"
FT                   /evidence="ECO:0000250"
FT   SITE            42
FT                   /note="Reactive site"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        105
FT                   /note="K -> N (in Ref. 2; AAA18557)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   135 AA;  14926 MW;  2C66485E6797CDF6 CRC64;
     MRKHRIVSLV AALLVLLALA AVSSTRSTQK ESVADNAGML AGGIKDVPAN ENDLQLQELA
     RFAVNEHNQK ANALLGFEKL VKAKTQVVAG TMYYLTIEVK DGEVKKLYEA KVWEKPWENF
     KQLQEFKPVE EGASA