CYT1_MOUSE
ID CYT1_MOUSE Reviewed; 97 AA.
AC P35175;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Stefin-1;
GN Name=Stfa1; Synonyms=Stf-1, Stf1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6J; TISSUE=Bone marrow;
RX PubMed=8468045; DOI=10.1006/geno.1993.1101;
RA Tsui F.W., Tsui H.W., Mok S., Mlinaric I., Copeland N.G., Gilbert D.J.,
RA Jenkins N.A., Siminovitch K.A.;
RT "Molecular characterization and mapping of murine genes encoding three
RT members of the stefin family of cysteine proteinase inhibitors.";
RL Genomics 15:507-514(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
CC -!- FUNCTION: This is an intracellular thiol proteinase inhibitor.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the cystatin family. {ECO:0000305}.
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DR EMBL; M92419; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; CT033776; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS37331.1; -.
DR PIR; C46011; C46011.
DR RefSeq; NP_001076012.1; NM_001082543.1.
DR AlphaFoldDB; P35175; -.
DR SMR; P35175; -.
DR STRING; 10090.ENSMUSP00000049068; -.
DR MEROPS; I25.001; -.
DR iPTMnet; P35175; -.
DR PhosphoSitePlus; P35175; -.
DR MaxQB; P35175; -.
DR PaxDb; P35175; -.
DR PRIDE; P35175; -.
DR ProteomicsDB; 277950; -.
DR Ensembl; ENSMUST00000232150; ENSMUSP00000156337; ENSMUSG00000071562.
DR GeneID; 20861; -.
DR KEGG; mmu:20861; -.
DR UCSC; uc007zck.1; mouse.
DR CTD; 20861; -.
DR MGI; MGI:106198; Stfa1.
DR VEuPathDB; HostDB:ENSMUSG00000071562; -.
DR GeneTree; ENSGT00940000155717; -.
DR HOGENOM; CLU_150234_2_1_1; -.
DR InParanoid; P35175; -.
DR OMA; NICDAMK; -.
DR OrthoDB; 1647595at2759; -.
DR PhylomeDB; P35175; -.
DR TreeFam; TF333174; -.
DR BioGRID-ORCS; 20861; 1 hit in 40 CRISPR screens.
DR ChiTaRS; Stfa1; mouse.
DR PRO; PR:P35175; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; P35175; protein.
DR Bgee; ENSMUSG00000071562; Expressed in granulocyte and 22 other tissues.
DR ExpressionAtlas; P35175; baseline and differential.
DR Genevisible; P35175; MM.
DR GO; GO:0001533; C:cornified envelope; ISO:MGI.
DR GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005615; C:extracellular space; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:1904090; C:peptidase inhibitor complex; ISO:MGI.
DR GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; ISO:MGI.
DR GO; GO:0004866; F:endopeptidase inhibitor activity; IDA:MGI.
DR GO; GO:0002020; F:protease binding; ISO:MGI.
DR GO; GO:0098609; P:cell-cell adhesion; ISO:MGI.
DR GO; GO:0030216; P:keratinocyte differentiation; ISO:MGI.
DR GO; GO:0010951; P:negative regulation of endopeptidase activity; IDA:MGI.
DR GO; GO:0010466; P:negative regulation of peptidase activity; ISO:MGI.
DR GO; GO:0045861; P:negative regulation of proteolysis; ISO:MGI.
DR GO; GO:0018149; P:peptide cross-linking; ISO:MGI.
DR CDD; cd00042; CY; 1.
DR InterPro; IPR000010; Cystatin_dom.
DR InterPro; IPR046350; Cystatin_sf.
DR InterPro; IPR018073; Prot_inh_cystat_CS.
DR InterPro; IPR001713; Prot_inh_stefin.
DR PANTHER; PTHR11414; PTHR11414; 1.
DR Pfam; PF00031; Cystatin; 1.
DR PRINTS; PR00295; STEFINA.
DR SMART; SM00043; CY; 1.
DR SUPFAM; SSF54403; SSF54403; 1.
DR PROSITE; PS00287; CYSTATIN; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Protease inhibitor; Reference proteome;
KW Thiol protease inhibitor.
FT CHAIN 1..97
FT /note="Stefin-1"
FT /id="PRO_0000207148"
FT MOTIF 46..50
FT /note="Secondary area of contact"
FT SITE 4
FT /note="Reactive site"
FT /evidence="ECO:0000250"
SQ SEQUENCE 97 AA; 11007 MW; 026A3D7FFE73CFBA CRC64;
MSLGGVSEAS RATPEIQMIA NKVRPQLEAK TNKKYEKFEA VEYKTQVVAG ENIFIKMDVG
HGCFIHIKVF NGPTGKDNYE LHGYQTDKTM DEELTYF
