ID   CYT24_CATRO             Reviewed;         499 AA.
AC   W8JMV1;
DT   10-APR-2019, integrated into UniProtKB/Swiss-Prot.
DT   14-MAY-2014, sequence version 1.
DT   03-AUG-2022, entry version 18.
DE   RecName: Full=Cytochrome P450 76T24 {ECO:0000303|PubMed:24710322};
DE            Short=CrCYP76T24 {ECO:0000303|PubMed:24710322};
DE            EC=1.14.-.- {ECO:0000305};
GN   Name=CYP76T24 {ECO:0000303|PubMed:24710322};
GN   ORFNames=Caros011349 {ECO:0000305};
OS   Catharanthus roseus (Madagascar periwinkle) (Vinca rosea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Gentianales; Apocynaceae; Rauvolfioideae; Vinceae;
OC   Catharanthinae; Catharanthus.
OX   NCBI_TaxID=4058;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Little Bright Eyes;
RX   PubMed=24710322; DOI=10.1038/ncomms4606;
RA   Miettinen K., Dong L., Navrot N., Schneider T., Burlat V., Pollier J.,
RA   Woittiez L., van der Krol S., Lugan R., Ilc T., Verpoorte R.,
RA   Oksman-Caldentey K.M., Martinoia E., Bouwmeester H., Goossens A.,
RA   Memelink J., Werck-Reichhart D.;
RT   "The seco-iridoid pathway from Catharanthus roseus.";
RL   Nat. Commun. 5:3606-3606(2014).
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass membrane
CC       protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
CC   -!- WEB RESOURCE: Name=ORCAE database;
CC       URL="https://bioinformatics.psb.ugent.be/orcae/overview/Catro";
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DR   EMBL; KF302075; AHK60842.1; -; mRNA.
DR   AlphaFoldDB; W8JMV1; -.
DR   SMR; W8JMV1; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Heme; Iron; Membrane; Metal-binding; Monooxygenase;
KW   Oxidoreductase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..499
FT                   /note="Cytochrome P450 76T24"
FT                   /id="PRO_0000446415"
FT   TRANSMEM        3..23
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   BINDING         442
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250|UniProtKB:P04798"
FT   CARBOHYD        55
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        76
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        279
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        284
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   499 AA;  56672 MW;  040C50E735BC2009 CRC64;
     MDVDILLSLV LAFFGWAAIY FLTSRLSFGK RARLPPGPYP FPVIGNIFQL GQNPNQSLTK
     LAKTYGPLMS LKLGSNTTVV VSSPAVAREV LQKNDQVFSS RIIPHAAEAH AHHKYSMVWL
     QVSGLWRNLR KISKEHMFAA QRLDASEGLR QEKLQELHDY LVRCSTSNKA VNFGQTAFTT
     SLNFISSTFF SVDFAAYGSD SSQEFKDIVW RILKSYSTPN VADYFPVLKF MDPQGILQKN
     TFLFSKMFDI FDNIINERLM MRGSLDTSKK NDLLEALLNH SANNESEFSL NELKHMLLDL
     FLGGTETTST TLEWAMAELL RNPEKLERVR AELHQVIGEK EIIHESDISR LPYLQAIVKE
     TFRLHPIVPL LIPHKAEADV EINGYTVPKN SQILINVWAS GRDSGTWLDP ETFSPERFLH
     SEIDMKGRHF ELIPFGAGRR ICPGLPLAYR TLHTMLATCI HNFDWKLKDG MKPEDIDMEE
     KYGLTLQLAV PLNVIPVKL