ID   CYT2_ARATH              Reviewed;         147 AA.
AC   Q8L5T9; Q9SL01;
DT   06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 2.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=Cysteine proteinase inhibitor 2;
DE            Short=AtCYS-2;
DE   Flags: Precursor;
GN   Name=CYS2; OrderedLocusNames=At2g31980; ORFNames=F22D22.27;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA   Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA   Feldmann K.A.;
RT   "Full-length cDNA from Arabidopsis thaliana.";
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   GENE FAMILY.
RX   PubMed=15887031; DOI=10.1007/s00438-005-1147-4;
RA   Martinez M., Abraham Z., Carbonero P., Diaz I.;
RT   "Comparative phylogenetic analysis of cystatin gene families from
RT   arabidopsis, rice and barley.";
RL   Mol. Genet. Genomics 273:423-432(2005).
CC   -!- FUNCTION: Specific inhibitor of cysteine proteinases. Probably involved
CC       in the regulation of endogenous processes and in defense against pests
CC       and pathogens (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the cystatin family. Phytocystatin subfamily.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAM64985.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AC006223; AAD15406.1; -; Genomic_DNA.
DR   EMBL; CP002685; AEC08615.1; -; Genomic_DNA.
DR   EMBL; AK229242; BAF01107.1; -; mRNA.
DR   EMBL; AY087439; AAM64985.1; ALT_INIT; mRNA.
DR   PIR; E84727; E84727.
DR   RefSeq; NP_180758.1; NM_128758.4.
DR   AlphaFoldDB; Q8L5T9; -.
DR   SMR; Q8L5T9; -.
DR   STRING; 3702.AT2G31980.1; -.
DR   MEROPS; I25.062; -.
DR   PaxDb; Q8L5T9; -.
DR   PRIDE; Q8L5T9; -.
DR   ProteomicsDB; 222751; -.
DR   EnsemblPlants; AT2G31980.1; AT2G31980.1; AT2G31980.
DR   GeneID; 817757; -.
DR   Gramene; AT2G31980.1; AT2G31980.1; AT2G31980.
DR   KEGG; ath:AT2G31980; -.
DR   Araport; AT2G31980; -.
DR   TAIR; locus:2045537; AT2G31980.
DR   eggNOG; ENOG502S4YZ; Eukaryota.
DR   HOGENOM; CLU_113093_0_0_1; -.
DR   InParanoid; Q8L5T9; -.
DR   OMA; THRGIHK; -.
DR   OrthoDB; 1565344at2759; -.
DR   PhylomeDB; Q8L5T9; -.
DR   PRO; PR:Q8L5T9; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; Q8L5T9; baseline and differential.
DR   Genevisible; Q8L5T9; AT.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   CDD; cd00042; CY; 1.
DR   InterPro; IPR000010; Cystatin_dom.
DR   InterPro; IPR046350; Cystatin_sf.
DR   InterPro; IPR018073; Prot_inh_cystat_CS.
DR   Pfam; PF16845; SQAPI; 1.
DR   SMART; SM00043; CY; 1.
DR   SUPFAM; SSF54403; SSF54403; 1.
DR   PROSITE; PS00287; CYSTATIN; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Plant defense; Protease inhibitor; Reference proteome;
KW   Secreted; Signal; Thiol protease inhibitor.
FT   SIGNAL          1..27
FT                   /evidence="ECO:0000255"
FT   CHAIN           28..147
FT                   /note="Cysteine proteinase inhibitor 2"
FT                   /id="PRO_0000277494"
FT   DOMAIN          87..117
FT                   /note="Cystatin"
FT   MOTIF           98..102
FT                   /note="Secondary area of contact"
FT                   /evidence="ECO:0000250"
FT   SITE            37
FT                   /note="Reactive site"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        115
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   147 AA;  16090 MW;  ABFD46F6E6622F0E CRC64;
     MATMLKVSLV LSLLGFLVIA VVTPSAANPF RKSVVLGGKS GVPNIRTNRE IQQLGRYCVE
     QFNQQAQNEQ GNIGSIAKTD TAISNPLQFS RVVSAQKQVV AGLKYYLRIE VTQPNGSTRM
     FDSVVVIQPW LHSKQLLGFT PVVSPVY