CYT2_MOUSE
ID CYT2_MOUSE Reviewed; 103 AA.
AC P35174; B2RQN4; E9QMN6;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2012, sequence version 2.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Stefin-2;
GN Name=Stfa2; Synonyms=Stf-2, Stf2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Bone marrow;
RX PubMed=8468045; DOI=10.1006/geno.1993.1101;
RA Tsui F.W., Tsui H.W., Mok S., Mlinaric I., Copeland N.G., Gilbert D.J.,
RA Jenkins N.A., Siminovitch K.A.;
RT "Molecular characterization and mapping of murine genes encoding three
RT members of the stefin family of cysteine proteinase inhibitors.";
RL Genomics 15:507-514(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: This is an intracellular thiol proteinase inhibitor.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=P35174-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P35174-2; Sequence=VSP_044325;
CC -!- SIMILARITY: Belongs to the cystatin family. {ECO:0000305}.
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DR EMBL; M92418; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; CT033776; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466521; EDK97921.1; -; Genomic_DNA.
DR EMBL; BC138004; AAI38005.1; -; mRNA.
DR CCDS; CCDS37334.1; -. [P35174-2]
DR PIR; B46011; B46011.
DR RefSeq; NP_001076014.1; NM_001082545.1. [P35174-2]
DR RefSeq; XP_006521994.1; XM_006521931.1.
DR AlphaFoldDB; P35174; -.
DR SMR; P35174; -.
DR STRING; 10090.ENSMUSP00000078181; -.
DR MEROPS; I25.001; -.
DR MaxQB; P35174; -.
DR PaxDb; P35174; -.
DR PRIDE; P35174; -.
DR ProteomicsDB; 279162; -. [P35174-1]
DR ProteomicsDB; 279163; -. [P35174-2]
DR DNASU; 20862; -.
DR Ensembl; ENSMUST00000023619; ENSMUSP00000023619; ENSMUSG00000022902. [P35174-2]
DR GeneID; 20862; -.
DR KEGG; mmu:20862; -.
DR UCSC; uc007zcn.1; mouse. [P35174-2]
DR CTD; 20862; -.
DR MGI; MGI:106197; Stfa2.
DR VEuPathDB; HostDB:ENSMUSG00000022902; -.
DR eggNOG; ENOG502SF2X; Eukaryota.
DR GeneTree; ENSGT00940000155717; -.
DR HOGENOM; CLU_150234_2_0_1; -.
DR InParanoid; P35174; -.
DR OMA; ICDKMKP; -.
DR OrthoDB; 1647595at2759; -.
DR TreeFam; TF333174; -.
DR BioGRID-ORCS; 20862; 2 hits in 36 CRISPR screens.
DR PRO; PR:P35174; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; P35174; protein.
DR Bgee; ENSMUSG00000022902; Expressed in granulocyte and 16 other tissues.
DR GO; GO:0001533; C:cornified envelope; ISO:MGI.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005615; C:extracellular space; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:1904090; C:peptidase inhibitor complex; ISO:MGI.
DR GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; ISO:MGI.
DR GO; GO:0004866; F:endopeptidase inhibitor activity; IDA:MGI.
DR GO; GO:0002020; F:protease binding; ISO:MGI.
DR GO; GO:0098609; P:cell-cell adhesion; ISO:MGI.
DR GO; GO:0030216; P:keratinocyte differentiation; ISO:MGI.
DR GO; GO:0010951; P:negative regulation of endopeptidase activity; IDA:MGI.
DR GO; GO:0010466; P:negative regulation of peptidase activity; ISO:MGI.
DR GO; GO:0045861; P:negative regulation of proteolysis; ISO:MGI.
DR GO; GO:0018149; P:peptide cross-linking; ISO:MGI.
DR CDD; cd00042; CY; 1.
DR InterPro; IPR000010; Cystatin_dom.
DR InterPro; IPR046350; Cystatin_sf.
DR InterPro; IPR018073; Prot_inh_cystat_CS.
DR InterPro; IPR001713; Prot_inh_stefin.
DR PANTHER; PTHR11414; PTHR11414; 1.
DR Pfam; PF00031; Cystatin; 1.
DR PRINTS; PR00295; STEFINA.
DR SMART; SM00043; CY; 1.
DR SUPFAM; SSF54403; SSF54403; 1.
DR PROSITE; PS00287; CYSTATIN; 1.
PE 3: Inferred from homology;
KW Alternative splicing; Cytoplasm; Protease inhibitor; Reference proteome;
KW Thiol protease inhibitor.
FT CHAIN 1..103
FT /note="Stefin-2"
FT /id="PRO_0000207147"
FT MOTIF 52..56
FT /note="Secondary area of contact"
FT SITE 10
FT /note="Reactive site"
FT /evidence="ECO:0000250"
FT VAR_SEQ 6..9
FT /note="RKIK -> IEII (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:8468045"
FT /id="VSP_044325"
FT CONFLICT 91..96
FT /note="QTNKAK -> KAKQTN (in Ref. 1; M92418)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 103 AA; 11842 MW; 80DB40A2410192C9 CRC64;
MTEYTRKIKG GLSEARPATS EIQEIADKVR PLLEEKTNEK YEKFKAIEYK VQVVQGLNYF
IKMNVGRGCY LHINVLSGIS SENDLELTGY QTNKAKNDEL TYF
