ID   CYT2_ORYSJ              Reviewed;         151 AA.
AC   P20907; B7E3T9; Q0DH35; Q4LG46; Q65X79;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 3.
DT   03-AUG-2022, entry version 124.
DE   RecName: Full=Cysteine proteinase inhibitor 2;
DE   AltName: Full=Oryzacystatin II;
DE            Short=OC-II;
DE   AltName: Full=Oryzacystatin-2;
GN   OrderedLocusNames=Os05g0494200, LOC_Os05g41460;
GN   ORFNames=OJ1579_G03.3, OsJ_19043 {ECO:0000312|EMBL:EEE64210.1};
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1993479; DOI=10.1016/0014-5793(91)80090-p;
RA   Kondo H., Abe K., Emori Y., Arai S.;
RT   "Gene organization of oryzacystatin-II, a new cystatin superfamily member
RT   of plant origin, is closely related to that of oryzacystatin-I but
RT   different from those of animal cystatins.";
RL   FEBS Lett. 278:87-90(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC   STRAIN=cv. Koshihikari;
RX   PubMed=15969500; DOI=10.1021/jf050348j;
RA   Ohtsubo S., Kobayashi H., Noro W., Taniguchi M., Saitoh E.;
RT   "Molecular cloning and characterization of oryzacystatin-III, a novel
RT   member of phytocystatin in rice (Oryza sativa L. japonica).";
RL   J. Agric. Food Chem. 53:5218-5224(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA   Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA   Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F.,
RA   Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT   "A fine physical map of the rice chromosome 5.";
RL   Mol. Genet. Genomics 274:337-345(2005).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [5]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [6]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [8]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [9]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 3-151, FUNCTION, AND DEVELOPMENTAL STAGE.
RC   TISSUE=Seed;
RX   PubMed=1697595; DOI=10.1016/s0021-9258(18)55473-9;
RA   Kondo H., Abe K., Nishimura I., Watanabe H., Emori Y., Arai S.;
RT   "Two distinct cystatin species in rice seeds with different specificities
RT   against cysteine proteinases. Molecular cloning, expression, and
RT   biochemical studies on oryzacystatin-II.";
RL   J. Biol. Chem. 265:15832-15837(1990).
RN   [10]
RP   GENE FAMILY.
RX   PubMed=15887031; DOI=10.1007/s00438-005-1147-4;
RA   Martinez M., Abraham Z., Carbonero P., Diaz I.;
RT   "Comparative phylogenetic analysis of cystatin gene families from
RT   arabidopsis, rice and barley.";
RL   Mol. Genet. Genomics 273:423-432(2005).
CC   -!- FUNCTION: There are two distinct cystatins in rice seeds
CC       (Oryzacystatin-1 and -2) with different specificities against cysteine
CC       proteinases. May be involved in the control of germination by
CC       inhibition of endogenous cysteine proteinases. May play a role in
CC       defense by inhibiting exogenous proteases such as those present in
CC       digestive tracks of insects and nematodes.
CC       {ECO:0000269|PubMed:1697595}.
CC   -!- DEVELOPMENTAL STAGE: Expressed in rice seeds from flowering to mature
CC       seeds. {ECO:0000269|PubMed:1697595}.
CC   -!- SIMILARITY: Belongs to the cystatin family. Phytocystatin subfamily.
CC       {ECO:0000305}.
CC   -!- CAUTION: PubMed:15969500 has erroneously named this protein
CC       oryzacystatin-3. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA33911.1; Type=Frameshift; Evidence={ECO:0000305};
CC       Sequence=BAE02682.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=BAF17838.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=CAA40860.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; X57658; CAA40860.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AB125972; BAE02681.1; -; mRNA.
DR   EMBL; AB125973; BAE02682.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AC112160; AAU44040.1; -; Genomic_DNA.
DR   EMBL; AP008211; BAF17838.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AP014961; BAS94690.1; -; Genomic_DNA.
DR   EMBL; CM000142; EEE64210.1; -; Genomic_DNA.
DR   EMBL; AK059563; BAG87036.1; -; mRNA.
DR   EMBL; J05595; AAA33911.1; ALT_SEQ; mRNA.
DR   PIR; A38375; A38375.
DR   PIR; S13027; S13027.
DR   RefSeq; XP_015639313.1; XM_015783827.1.
DR   AlphaFoldDB; P20907; -.
DR   SMR; P20907; -.
DR   STRING; 4530.OS05T0494200-02; -.
DR   MEROPS; I25.028; -.
DR   PaxDb; P20907; -.
DR   PRIDE; P20907; -.
DR   EnsemblPlants; Os05t0494200-02; Os05t0494200-02; Os05g0494200.
DR   GeneID; 4339190; -.
DR   Gramene; Os05t0494200-02; Os05t0494200-02; Os05g0494200.
DR   KEGG; osa:4339190; -.
DR   eggNOG; ENOG502R3J4; Eukaryota.
DR   HOGENOM; CLU_113093_1_1_1; -.
DR   InParanoid; P20907; -.
DR   OMA; WERAWEN; -.
DR   OrthoDB; 1565344at2759; -.
DR   Proteomes; UP000000763; Chromosome 5.
DR   Proteomes; UP000007752; Chromosome 5.
DR   Proteomes; UP000059680; Chromosome 5.
DR   ExpressionAtlas; P20907; baseline and differential.
DR   Genevisible; P20907; OS.
DR   GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IBA:GO_Central.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   CDD; cd00042; CY; 1.
DR   InterPro; IPR027214; Cystatin.
DR   InterPro; IPR000010; Cystatin_dom.
DR   InterPro; IPR046350; Cystatin_sf.
DR   InterPro; IPR018073; Prot_inh_cystat_CS.
DR   PANTHER; PTHR11413; PTHR11413; 1.
DR   Pfam; PF16845; SQAPI; 1.
DR   SMART; SM00043; CY; 1.
DR   SUPFAM; SSF54403; SSF54403; 1.
DR   PROSITE; PS00287; CYSTATIN; 1.
PE   2: Evidence at transcript level;
KW   Plant defense; Protease inhibitor; Reference proteome;
KW   Thiol protease inhibitor.
FT   CHAIN           1..151
FT                   /note="Cysteine proteinase inhibitor 2"
FT                   /id="PRO_0000207157"
FT   DOMAIN          57..147
FT                   /note="Cystatin"
FT   MOTIF           102..106
FT                   /note="Secondary area of contact"
FT                   /evidence="ECO:0000250"
FT   SITE            57
FT                   /note="Reactive site"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        148
FT                   /note="Missing (in Ref. 1; CAA40860)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   151 AA;  16561 MW;  D4788F5B424F50B7 CRC64;
     MRASSLFAES VFTTSAAAGR RRCPRLAAVP VTLFFSTGRG SPAMAEEAQQ PRGVKVGGIH
     DAPAGRENDL TTVELARFAV AEHNSKANAM LELERVVKVR QQVVGGFMHY LTVEVKEPGG
     ANKLYEAKVW ERAWENFKQL QDFKPLDDAT A