CYT3_ARATH
ID CYT3_ARATH Reviewed; 125 AA.
AC Q41906; O22202; Q8RXS7; Q9FXN6;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 2.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Cysteine proteinase inhibitor 3;
DE Short=AtCYS-3;
DE Flags: Precursor;
GN Name=CYS3; Synonyms=FL3-27; OrderedLocusNames=At2g40880; ORFNames=T20B5.8;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=8281187; DOI=10.1046/j.1365-313x.1993.04061051.x;
RA Hoefte H., Desprez T., Amselem J., Chiapello H., Rouze P., Caboche M.,
RA Moisan A., Jourjon M.-F., Charpenteau J.-L., Berthomieu P., Guerrier D.,
RA Giraudat J., Quigley F., Thomas F., Yu D.-Y., Mache R., Raynal M.,
RA Cooke R., Grellet F., Delseny M., Parmentier Y., de Marcillac G., Gigot C.,
RA Fleck J., Philipps G., Axelos M., Bardet C., Tremousaygue D., Lescure B.;
RT "An inventory of 1152 expressed sequence tags obtained by partial
RT sequencing of cDNAs from Arabidopsis thaliana.";
RL Plant J. 4:1051-1061(1993).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 10-125.
RX PubMed=11158529; DOI=10.2307/3871153;
RA Seki M., Narusaka M., Abe H., Kasuga M., Yamaguchi-Shinozaki K.,
RA Carninci P., Hayashizaki Y., Shinozaki K.;
RT "Monitoring the expression pattern of 1300 Arabidopsis genes under drought
RT and cold stresses by using a full-length cDNA microarray.";
RL Plant Cell 13:61-72(2001).
RN [7]
RP GENE FAMILY.
RX PubMed=15887031; DOI=10.1007/s00438-005-1147-4;
RA Martinez M., Abraham Z., Carbonero P., Diaz I.;
RT "Comparative phylogenetic analysis of cystatin gene families from
RT arabidopsis, rice and barley.";
RL Mol. Genet. Genomics 273:423-432(2005).
CC -!- FUNCTION: Specific inhibitor of cysteine proteinases. Probably involved
CC in the regulation of endogenous processes and in defense against pests
CC and pathogens (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the cystatin family. Phytocystatin subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB17683.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAA79016.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AC002409; AAB86448.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC09892.1; -; Genomic_DNA.
DR EMBL; AY080692; AAL86314.1; -; mRNA.
DR EMBL; AY150484; AAN13009.1; -; mRNA.
DR EMBL; Z17618; CAA79016.1; ALT_FRAME; mRNA.
DR EMBL; AY084769; AAM61337.1; -; mRNA.
DR EMBL; AB044405; BAB17683.1; ALT_INIT; mRNA.
DR PIR; T00752; T00752.
DR RefSeq; NP_181620.1; NM_129651.4.
DR AlphaFoldDB; Q41906; -.
DR SMR; Q41906; -.
DR BioGRID; 4022; 1.
DR IntAct; Q41906; 1.
DR STRING; 3702.AT2G40880.1; -.
DR MEROPS; I25.014; -.
DR PaxDb; Q41906; -.
DR PRIDE; Q41906; -.
DR ProteomicsDB; 224659; -.
DR EnsemblPlants; AT2G40880.1; AT2G40880.1; AT2G40880.
DR GeneID; 818685; -.
DR Gramene; AT2G40880.1; AT2G40880.1; AT2G40880.
DR KEGG; ath:AT2G40880; -.
DR Araport; AT2G40880; -.
DR TAIR; locus:2058515; AT2G40880.
DR eggNOG; ENOG502SC50; Eukaryota.
DR HOGENOM; CLU_113093_1_1_1; -.
DR InParanoid; Q41906; -.
DR OMA; AKVWEQP; -.
DR OrthoDB; 1565344at2759; -.
DR PhylomeDB; Q41906; -.
DR PRO; PR:Q41906; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q41906; baseline and differential.
DR Genevisible; Q41906; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IDA:TAIR.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0006972; P:hyperosmotic response; IMP:TAIR.
DR GO; GO:0009409; P:response to cold; IMP:TAIR.
DR GO; GO:0006979; P:response to oxidative stress; IMP:TAIR.
DR GO; GO:0009414; P:response to water deprivation; IMP:TAIR.
DR CDD; cd00042; CY; 1.
DR InterPro; IPR027214; Cystatin.
DR InterPro; IPR000010; Cystatin_dom.
DR InterPro; IPR046350; Cystatin_sf.
DR InterPro; IPR018073; Prot_inh_cystat_CS.
DR PANTHER; PTHR11413; PTHR11413; 1.
DR Pfam; PF16845; SQAPI; 1.
DR SMART; SM00043; CY; 1.
DR SUPFAM; SSF54403; SSF54403; 1.
DR PROSITE; PS00287; CYSTATIN; 1.
PE 2: Evidence at transcript level;
KW Plant defense; Protease inhibitor; Reference proteome; Secreted; Signal;
KW Thiol protease inhibitor.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..125
FT /note="Cysteine proteinase inhibitor 3"
FT /id="PRO_0000277495"
FT DOMAIN 36..124
FT /note="Cystatin"
FT MOTIF 80..84
FT /note="Secondary area of contact"
FT /evidence="ECO:0000250"
FT SITE 36
FT /note="Reactive site"
FT /evidence="ECO:0000250"
SQ SEQUENCE 125 AA; 14422 MW; E6AB183B8B6F778E CRC64;
MESKTFWIVT LLLCGTIQLA ICRSEEKSTE KTMMLGGVHD LRGNQNSGEI ESLARFAIQE
HNKQQNKILE FKKIVKAREQ VVAGTMYHLT LEAKEGDQTK NFEAKVWVKP WMNFKQLQEF
KESSS
