CYT3_MOUSE
ID CYT3_MOUSE Reviewed; 103 AA.
AC P35173; Q540I0;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Stefin-3;
GN Name=Stfa3; Synonyms=Stf-3, Stf3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6J; TISSUE=Bone marrow;
RX PubMed=8468045; DOI=10.1006/geno.1993.1101;
RA Tsui F.W., Tsui H.W., Mok S., Mlinaric I., Copeland N.G., Gilbert D.J.,
RA Jenkins N.A., Siminovitch K.A.;
RT "Molecular characterization and mapping of murine genes encoding three
RT members of the stefin family of cysteine proteinase inhibitors.";
RL Genomics 15:507-514(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=A/J; TISSUE=Spleen;
RA Gao J., Teuscher C.;
RL Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: This is an intracellular thiol proteinase inhibitor.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the cystatin family. {ECO:0000305}.
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DR EMBL; M92417; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AY169236; AAN87899.1; -; mRNA.
DR EMBL; AK014349; BAB29290.1; -; mRNA.
DR EMBL; AC074229; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466521; EDK97922.1; -; Genomic_DNA.
DR EMBL; BC107224; AAI07225.1; -; mRNA.
DR EMBL; BC107225; AAI07226.1; -; mRNA.
DR CCDS; CCDS28153.1; -.
DR PIR; A46011; A46011.
DR RefSeq; NP_079564.1; NM_025288.2.
DR AlphaFoldDB; P35173; -.
DR SMR; P35173; -.
DR STRING; 10090.ENSMUSP00000067445; -.
DR MEROPS; I25.001; -.
DR iPTMnet; P35173; -.
DR PhosphoSitePlus; P35173; -.
DR PaxDb; P35173; -.
DR PRIDE; P35173; -.
DR ProteomicsDB; 279164; -.
DR DNASU; 20863; -.
DR Ensembl; ENSMUST00000068182; ENSMUSP00000067445; ENSMUSG00000054905.
DR GeneID; 20863; -.
DR KEGG; mmu:20863; -.
DR UCSC; uc007zco.1; mouse.
DR CTD; 20863; -.
DR MGI; MGI:106196; Stfa3.
DR VEuPathDB; HostDB:ENSMUSG00000054905; -.
DR eggNOG; ENOG502SF2X; Eukaryota.
DR GeneTree; ENSGT00940000155717; -.
DR HOGENOM; CLU_150234_2_0_1; -.
DR InParanoid; P35173; -.
DR OMA; HLRIFQA; -.
DR OrthoDB; 1647595at2759; -.
DR PhylomeDB; P35173; -.
DR TreeFam; TF333174; -.
DR Reactome; R-MMU-6809371; Formation of the cornified envelope.
DR BioGRID-ORCS; 20863; 2 hits in 51 CRISPR screens.
DR ChiTaRS; Stfa3; mouse.
DR PRO; PR:P35173; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; P35173; protein.
DR Bgee; ENSMUSG00000054905; Expressed in umbilical cord and 65 other tissues.
DR Genevisible; P35173; MM.
DR GO; GO:0001533; C:cornified envelope; ISO:MGI.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005615; C:extracellular space; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:1904090; C:peptidase inhibitor complex; ISO:MGI.
DR GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; ISO:MGI.
DR GO; GO:0002020; F:protease binding; ISO:MGI.
DR GO; GO:0098609; P:cell-cell adhesion; ISO:MGI.
DR GO; GO:0030216; P:keratinocyte differentiation; ISO:MGI.
DR GO; GO:0010466; P:negative regulation of peptidase activity; ISO:MGI.
DR GO; GO:0045861; P:negative regulation of proteolysis; ISO:MGI.
DR GO; GO:0018149; P:peptide cross-linking; ISO:MGI.
DR CDD; cd00042; CY; 1.
DR InterPro; IPR000010; Cystatin_dom.
DR InterPro; IPR046350; Cystatin_sf.
DR InterPro; IPR018073; Prot_inh_cystat_CS.
DR InterPro; IPR001713; Prot_inh_stefin.
DR PANTHER; PTHR11414; PTHR11414; 1.
DR Pfam; PF00031; Cystatin; 1.
DR PRINTS; PR00295; STEFINA.
DR SMART; SM00043; CY; 1.
DR SUPFAM; SSF54403; SSF54403; 1.
DR PROSITE; PS00287; CYSTATIN; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Protease inhibitor; Reference proteome;
KW Thiol protease inhibitor.
FT CHAIN 1..103
FT /note="Stefin-3"
FT /id="PRO_0000207146"
FT MOTIF 52..56
FT /note="Secondary area of contact"
FT SITE 10
FT /note="Reactive site"
FT /evidence="ECO:0000250"
SQ SEQUENCE 103 AA; 11640 MW; 2E97495F71022C8E CRC64;
MSQENLKIKG GLSEARPATP EIQMIADKVR PLLEEQTNEK YEKFEAVEYK SQVVAGQNLF
IKIDVGNGCF LHMKVFRGLS GEDDLKLKGY QTNKTKTDEL TSM
