CYT5_ARATH
ID CYT5_ARATH Reviewed; 122 AA.
AC Q41916; Q8LBN6; Q9FGK2;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 2.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Cysteine proteinase inhibitor 5;
DE Short=AtCYS-5;
DE Flags: Precursor;
GN Name=CYS5; OrderedLocusNames=At5g47550; ORFNames=MNJ7.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
RL Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=8281187; DOI=10.1046/j.1365-313x.1993.04061051.x;
RA Hoefte H., Desprez T., Amselem J., Chiapello H., Rouze P., Caboche M.,
RA Moisan A., Jourjon M.-F., Charpenteau J.-L., Berthomieu P., Guerrier D.,
RA Giraudat J., Quigley F., Thomas F., Yu D.-Y., Mache R., Raynal M.,
RA Cooke R., Grellet F., Delseny M., Parmentier Y., de Marcillac G., Gigot C.,
RA Fleck J., Philipps G., Axelos M., Bardet C., Tremousaygue D., Lescure B.;
RT "An inventory of 1152 expressed sequence tags obtained by partial
RT sequencing of cDNAs from Arabidopsis thaliana.";
RL Plant J. 4:1051-1061(1993).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY.
RX PubMed=15887031; DOI=10.1007/s00438-005-1147-4;
RA Martinez M., Abraham Z., Carbonero P., Diaz I.;
RT "Comparative phylogenetic analysis of cystatin gene families from
RT arabidopsis, rice and barley.";
RL Mol. Genet. Genomics 273:423-432(2005).
CC -!- FUNCTION: Specific inhibitor of cysteine proteinases. Probably involved
CC in the regulation of endogenous processes and in defense against pests
CC and pathogens (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the cystatin family. Phytocystatin subfamily.
CC {ECO:0000305}.
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DR EMBL; AB025628; BAB09081.1; -; Genomic_DNA.
DR EMBL; CP002688; AED95531.1; -; Genomic_DNA.
DR EMBL; AY072354; AAL62346.1; -; mRNA.
DR EMBL; BT006535; AAP21343.1; -; mRNA.
DR EMBL; Z17675; CAA79032.1; -; mRNA.
DR EMBL; AY087102; AAM64661.1; -; mRNA.
DR RefSeq; NP_199566.1; NM_124128.2.
DR AlphaFoldDB; Q41916; -.
DR SMR; Q41916; -.
DR STRING; 3702.AT5G47550.1; -.
DR MEROPS; I25.033; -.
DR PaxDb; Q41916; -.
DR PRIDE; Q41916; -.
DR ProteomicsDB; 222752; -.
DR EnsemblPlants; AT5G47550.1; AT5G47550.1; AT5G47550.
DR GeneID; 834805; -.
DR Gramene; AT5G47550.1; AT5G47550.1; AT5G47550.
DR KEGG; ath:AT5G47550; -.
DR Araport; AT5G47550; -.
DR TAIR; locus:2168973; AT5G47550.
DR eggNOG; ENOG502S46Q; Eukaryota.
DR HOGENOM; CLU_113093_5_0_1; -.
DR InParanoid; Q41916; -.
DR OMA; SFEPANN; -.
DR OrthoDB; 1565344at2759; -.
DR PhylomeDB; Q41916; -.
DR PRO; PR:Q41916; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q41916; baseline and differential.
DR Genevisible; Q41916; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR GO; GO:0034605; P:cellular response to heat; IMP:TAIR.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0010030; P:positive regulation of seed germination; IMP:TAIR.
DR CDD; cd00042; CY; 1.
DR InterPro; IPR000010; Cystatin_dom.
DR InterPro; IPR046350; Cystatin_sf.
DR Pfam; PF16845; SQAPI; 1.
DR SMART; SM00043; CY; 1.
DR SUPFAM; SSF54403; SSF54403; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Plant defense; Protease inhibitor; Reference proteome;
KW Secreted; Signal; Thiol protease inhibitor.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..122
FT /note="Cysteine proteinase inhibitor 5"
FT /id="PRO_0000277497"
FT DOMAIN 29..117
FT /note="Cystatin"
FT MOTIF 72..76
FT /note="Secondary area of contact"
FT /evidence="ECO:0000250"
FT SITE 29
FT /note="Reactive site"
FT /evidence="ECO:0000250"
FT CARBOHYD 36
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 109
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 16
FT /note="V -> L (in Ref. 5; AAM64661)"
FT /evidence="ECO:0000305"
FT CONFLICT 101
FT /note="D -> V (in Ref. 4; CAA79032)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 122 AA; 13372 MW; 970AF2D07DF087FF CRC64;
MTSKVVFLLL LSLVVVLLPL YASAAARVGG WSPISNVTDP QVVEIGEFAV SEYNKRSESG
LKFETVVSGE TQVVSGTNYR LKVAANDGDG VSKNYLAIVW DKPWMKFRNL TSFEPANNGR
FL
