ID   CYTA1_DICDI             Reviewed;          94 AA.
AC   Q65YR8; Q54E27;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2004, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Cystatin-A1;
GN   Name=cpiA; ORFNames=DDB_G0291834;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND
RP   DEVELOPMENTAL STAGE.
RC   STRAIN=AX4;
RX   PubMed=15255188; DOI=10.1515/bc.2004.065;
RA   El-Halawany M.S., Ohkouchi S., Shibata H., Hitomi K., Maki M.;
RT   "Identification of cysteine protease inhibitors that belong to cystatin
RT   family 1 in the cellular slime mold Dictyostelium discoideum.";
RL   Biol. Chem. 385:547-550(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Intracellular thiol proteinase inhibitor. Inhibits papain,
CC       but not cathepsin B. {ECO:0000269|PubMed:15255188}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:15255188}.
CC   -!- DEVELOPMENTAL STAGE: Stably expressed throughout development.
CC       {ECO:0000269|PubMed:15255188}.
CC   -!- SIMILARITY: Belongs to the cystatin family. {ECO:0000305}.
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DR   EMBL; AB189918; BAD44693.1; -; mRNA.
DR   EMBL; AAFI02000185; EAL61538.1; -; Genomic_DNA.
DR   RefSeq; XP_629960.1; XM_629958.1.
DR   AlphaFoldDB; Q65YR8; -.
DR   SMR; Q65YR8; -.
DR   BioGRID; 1253369; 1.
DR   STRING; 44689.DDB0220657; -.
DR   PaxDb; Q65YR8; -.
DR   EnsemblProtists; EAL61538; EAL61538; DDB_G0291834.
DR   GeneID; 8628366; -.
DR   KEGG; ddi:DDB_G0291834; -.
DR   dictyBase; DDB_G0291834; cpiA.
DR   eggNOG; ENOG502RIJB; Eukaryota.
DR   HOGENOM; CLU_150234_2_0_1; -.
DR   InParanoid; Q65YR8; -.
DR   OMA; AAYQTNK; -.
DR   PhylomeDB; Q65YR8; -.
DR   Reactome; R-DDI-6798695; Neutrophil degranulation.
DR   PRO; PR:Q65YR8; -.
DR   Proteomes; UP000002195; Chromosome 6.
DR   GO; GO:0005829; C:cytosol; IDA:dictyBase.
DR   GO; GO:0045335; C:phagocytic vesicle; HDA:dictyBase.
DR   GO; GO:0031982; C:vesicle; IDA:dictyBase.
DR   GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IDA:dictyBase.
DR   GO; GO:0052547; P:regulation of peptidase activity; IDA:dictyBase.
DR   GO; GO:0009617; P:response to bacterium; HEP:dictyBase.
DR   InterPro; IPR000010; Cystatin_dom.
DR   InterPro; IPR046350; Cystatin_sf.
DR   InterPro; IPR018073; Prot_inh_cystat_CS.
DR   InterPro; IPR001713; Prot_inh_stefin.
DR   PANTHER; PTHR11414; PTHR11414; 1.
DR   Pfam; PF00031; Cystatin; 1.
DR   SUPFAM; SSF54403; SSF54403; 1.
DR   PROSITE; PS00287; CYSTATIN; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Protease inhibitor; Reference proteome;
KW   Thiol protease inhibitor.
FT   CHAIN           1..94
FT                   /note="Cystatin-A1"
FT                   /id="PRO_0000327785"
FT   MOTIF           45..49
FT                   /note="Secondary area of contact"
FT                   /evidence="ECO:0000250"
FT   SITE            5
FT                   /note="Reactive site"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   94 AA;  10401 MW;  8D03C836DD6B5E4B CRC64;
     MTLGGLKPEV HAANDEIRQV VAKVADELKS KLNTTEVEPV SYKTQLVAGT NYFIKVKTPA
     GFAHARVYKD LQQNHSVHSV KADGITEESE IVYF