ID   CYTA2_DICDI             Reviewed;          95 AA.
AC   Q65YR7; Q54VD4;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2004, sequence version 1.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=Cystatin-A2;
GN   Name=cpiB; ORFNames=DDB_G0280439;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND
RP   DEVELOPMENTAL STAGE.
RC   STRAIN=AX4;
RX   PubMed=15255188; DOI=10.1515/bc.2004.065;
RA   El-Halawany M.S., Ohkouchi S., Shibata H., Hitomi K., Maki M.;
RT   "Identification of cysteine protease inhibitors that belong to cystatin
RT   family 1 in the cellular slime mold Dictyostelium discoideum.";
RL   Biol. Chem. 385:547-550(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Intracellular thiol proteinase inhibitor. Inhibits cathepsin
CC       B, but not papain. {ECO:0000269|PubMed:15255188}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:15255188}.
CC   -!- DEVELOPMENTAL STAGE: First detected at the aggregation stage and peaks
CC       during culmination. {ECO:0000269|PubMed:15255188}.
CC   -!- SIMILARITY: Belongs to the cystatin family. {ECO:0000305}.
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DR   EMBL; AB189919; BAD44694.1; -; mRNA.
DR   EMBL; AAFI02000036; EAL67198.1; -; Genomic_DNA.
DR   RefSeq; XP_641175.1; XM_636083.1.
DR   AlphaFoldDB; Q65YR7; -.
DR   SMR; Q65YR7; -.
DR   STRING; 44689.DDB0220658; -.
DR   PaxDb; Q65YR7; -.
DR   EnsemblProtists; EAL67198; EAL67198; DDB_G0280439.
DR   GeneID; 8622555; -.
DR   KEGG; ddi:DDB_G0280439; -.
DR   dictyBase; DDB_G0280439; cpiB.
DR   eggNOG; ENOG502SF2X; Eukaryota.
DR   HOGENOM; CLU_150234_2_0_1; -.
DR   InParanoid; Q65YR7; -.
DR   OMA; GATHQAD; -.
DR   PhylomeDB; Q65YR7; -.
DR   Reactome; R-DDI-6798695; Neutrophil degranulation.
DR   PRO; PR:Q65YR7; -.
DR   Proteomes; UP000002195; Chromosome 3.
DR   GO; GO:0005829; C:cytosol; IDA:dictyBase.
DR   GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IDA:dictyBase.
DR   GO; GO:0052547; P:regulation of peptidase activity; IDA:dictyBase.
DR   InterPro; IPR000010; Cystatin_dom.
DR   InterPro; IPR046350; Cystatin_sf.
DR   InterPro; IPR001713; Prot_inh_stefin.
DR   PANTHER; PTHR11414; PTHR11414; 1.
DR   Pfam; PF00031; Cystatin; 1.
DR   PRINTS; PR00295; STEFINA.
DR   SUPFAM; SSF54403; SSF54403; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Protease inhibitor; Reference proteome;
KW   Thiol protease inhibitor.
FT   CHAIN           1..95
FT                   /note="Cystatin-A2"
FT                   /id="PRO_0000327786"
FT   MOTIF           47..51
FT                   /note="Secondary area of contact"
FT                   /evidence="ECO:0000250"
FT   SITE            5
FT                   /note="Reactive site"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   95 AA;  10351 MW;  8255C5A257B3CDBB CRC64;
     MTKVGGLGAT HQADKTVEDI VNAVKPSIQS KLGTNISNLK VISYKTQLVN GTNYFVKVRT
     ENGYAHLRIY KPFSGAASLV SVQDGKAKDD EITYF