ID   CYTA3_DICDI             Reviewed;          94 AA.
AC   Q5R1U3; Q550T7; Q5R1U2; Q86JB2;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   04-JAN-2005, sequence version 1.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=Cystatin-A3;
DE   AltName: Full=VSA745;
GN   Name=cpiC; ORFNames=DDB_G0277001;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC   STRAIN=AX2, and AX4;
RX   PubMed=16328887; DOI=10.1007/s11033-005-3139-1;
RA   El-Halawany M.S., Shibata H., Hitomi K., Maki M.;
RT   "Reevaluation of the predicted gene structure of Dictyostelium cystatin A3
RT   (cpiC) by nucleotide sequence determination of its cDNA and its
RT   phylogenetic position in the cystatin superfamily.";
RL   Mol. Biol. Rep. 32:257-264(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=12097910; DOI=10.1038/nature00847;
RA   Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA   Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA   Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA   Noegel A.A.;
RT   "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL   Nature 418:79-85(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [4]
RP   IDENTIFICATION.
RX   PubMed=15255188; DOI=10.1515/bc.2004.065;
RA   El-Halawany M.S., Ohkouchi S., Shibata H., Hitomi K., Maki M.;
RT   "Identification of cysteine protease inhibitors that belong to cystatin
RT   family 1 in the cellular slime mold Dictyostelium discoideum.";
RL   Biol. Chem. 385:547-550(2004).
CC   -!- FUNCTION: Intracellular thiol proteinase inhibitor. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the cystatin family. {ECO:0000305}.
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DR   EMBL; AB189920; BAD77797.1; -; mRNA.
DR   EMBL; AB189921; BAD77798.1; -; Genomic_DNA.
DR   EMBL; AAFI02000019; EAL69002.2; -; Genomic_DNA.
DR   RefSeq; XP_642845.2; XM_637753.2.
DR   AlphaFoldDB; Q5R1U3; -.
DR   SMR; Q5R1U3; -.
DR   STRING; 44689.DDB0252666; -.
DR   PaxDb; Q5R1U3; -.
DR   EnsemblProtists; EAL69002; EAL69002; DDB_G0277001.
DR   GeneID; 8620709; -.
DR   KEGG; ddi:DDB_G0277001; -.
DR   dictyBase; DDB_G0277001; cpiC.
DR   eggNOG; ENOG502SF2X; Eukaryota.
DR   HOGENOM; CLU_150234_2_1_1; -.
DR   InParanoid; Q5R1U3; -.
DR   OMA; ICDKMKP; -.
DR   PhylomeDB; Q5R1U3; -.
DR   Reactome; R-DDI-6798695; Neutrophil degranulation.
DR   PRO; PR:Q5R1U3; -.
DR   Proteomes; UP000002195; Chromosome 2.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; ISS:dictyBase.
DR   GO; GO:0006972; P:hyperosmotic response; IEP:dictyBase.
DR   GO; GO:0052547; P:regulation of peptidase activity; ISS:dictyBase.
DR   CDD; cd00042; CY; 1.
DR   InterPro; IPR000010; Cystatin_dom.
DR   InterPro; IPR046350; Cystatin_sf.
DR   InterPro; IPR001713; Prot_inh_stefin.
DR   PANTHER; PTHR11414; PTHR11414; 1.
DR   Pfam; PF00031; Cystatin; 1.
DR   PRINTS; PR00295; STEFINA.
DR   SMART; SM00043; CY; 1.
DR   SUPFAM; SSF54403; SSF54403; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Protease inhibitor; Reference proteome;
KW   Thiol protease inhibitor.
FT   CHAIN           1..94
FT                   /note="Cystatin-A3"
FT                   /id="PRO_0000327787"
FT   MOTIF           46..50
FT                   /note="Secondary area of contact"
FT                   /evidence="ECO:0000250"
FT   SITE            3
FT                   /note="Reactive site"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   94 AA;  10739 MW;  21361BD067112753 CRC64;
     MTGGISPHPI NATPEIQEIT NTVKEQLEKK LGTNYSIFQA ISYKKQLVNG MNYFIKVKTD
     NGYDHIRVYE AFKGTPNLVS VQQHKSLEDD ITYF