ID   CYTA_RAT                Reviewed;         103 AA.
AC   P01039;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   03-AUG-2022, entry version 144.
DE   RecName: Full=Cystatin-A;
DE   AltName: Full=Cystatin-alpha;
DE   AltName: Full=Epidermal stefin;
DE   AltName: Full=Epidermal thiol proteinase inhibitor;
GN   Name=Csta;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   PROTEIN SEQUENCE, AND ACETYLATION AT MET-1.
RX   PubMed=6732797; DOI=10.1016/0006-291x(84)90699-5;
RA   Takio K., Kominami E., Bando Y., Katunuma N., Titani K.;
RT   "Amino acid sequence of rat epidermal thiol proteinase inhibitor.";
RL   Biochem. Biophys. Res. Commun. 121:149-154(1984).
RN   [2]
RP   PROTEIN SEQUENCE OF 7-10.
RX   PubMed=2205237;
RA   Takeda A., Nakamura Y., Kaji H., Samejima T.;
RT   "Evidence of protease activity producing NH2-terminal truncated form of rat
RT   cystatin A in newborn rat epidermis.";
RL   Biol. Chem. Hoppe-Seyler 371:233-238(1990).
CC   -!- FUNCTION: This is an intracellular thiol proteinase inhibitor.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the cystatin family. {ECO:0000305}.
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DR   PIR; A01275; UDRTS2.
DR   RefSeq; NP_001099347.1; NM_001105877.1.
DR   AlphaFoldDB; P01039; -.
DR   SMR; P01039; -.
DR   STRING; 10116.ENSRNOP00000043059; -.
DR   MEROPS; I25.001; -.
DR   iPTMnet; P01039; -.
DR   PhosphoSitePlus; P01039; -.
DR   PaxDb; P01039; -.
DR   Ensembl; ENSRNOT00000051936; ENSRNOP00000043059; ENSRNOG00000033933.
DR   GeneID; 288075; -.
DR   KEGG; rno:288075; -.
DR   UCSC; RGD:1309603; rat.
DR   CTD; 20863; -.
DR   RGD; 1309603; Csta.
DR   eggNOG; ENOG502SF2X; Eukaryota.
DR   GeneTree; ENSGT00940000155717; -.
DR   HOGENOM; CLU_150234_2_0_1; -.
DR   InParanoid; P01039; -.
DR   OMA; IHIRVFE; -.
DR   OrthoDB; 1647595at2759; -.
DR   PhylomeDB; P01039; -.
DR   TreeFam; TF333174; -.
DR   Reactome; R-RNO-6809371; Formation of the cornified envelope.
DR   PRO; PR:P01039; -.
DR   Proteomes; UP000002494; Chromosome 11.
DR   Bgee; ENSRNOG00000033933; Expressed in esophagus and 11 other tissues.
DR   Genevisible; P01039; RN.
DR   GO; GO:0001533; C:cornified envelope; ISO:RGD.
DR   GO; GO:0005737; C:cytoplasm; ISO:RGD.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005615; C:extracellular space; ISO:RGD.
DR   GO; GO:1904090; C:peptidase inhibitor complex; ISO:RGD.
DR   GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; ISO:RGD.
DR   GO; GO:0002020; F:protease binding; ISO:RGD.
DR   GO; GO:0098609; P:cell-cell adhesion; ISO:RGD.
DR   GO; GO:0030216; P:keratinocyte differentiation; ISO:RGD.
DR   GO; GO:0010466; P:negative regulation of peptidase activity; ISO:RGD.
DR   GO; GO:0045861; P:negative regulation of proteolysis; ISO:RGD.
DR   GO; GO:0018149; P:peptide cross-linking; ISO:RGD.
DR   CDD; cd00042; CY; 1.
DR   InterPro; IPR000010; Cystatin_dom.
DR   InterPro; IPR046350; Cystatin_sf.
DR   InterPro; IPR018073; Prot_inh_cystat_CS.
DR   InterPro; IPR001713; Prot_inh_stefin.
DR   PANTHER; PTHR11414; PTHR11414; 1.
DR   Pfam; PF00031; Cystatin; 1.
DR   PRINTS; PR00295; STEFINA.
DR   SMART; SM00043; CY; 1.
DR   SUPFAM; SSF54403; SSF54403; 1.
DR   PROSITE; PS00287; CYSTATIN; 1.
PE   1: Evidence at protein level;
KW   Acetylation; Cytoplasm; Direct protein sequencing; Protease inhibitor;
KW   Reference proteome; Thiol protease inhibitor.
FT   CHAIN           1..103
FT                   /note="Cystatin-A"
FT                   /id="PRO_0000207129"
FT   MOTIF           52..56
FT                   /note="Secondary area of contact"
FT   SITE            10
FT                   /note="Reactive site"
FT   MOD_RES         1
FT                   /note="N-acetylmethionine"
FT                   /evidence="ECO:0000269|PubMed:6732797"
SQ   SEQUENCE   103 AA;  11563 MW;  079D36FA32A5EEE6 CRC64;
     MDPGTTGIVG GVSEAKPATP EIQEVADKVK RQLEEKTNEK YEKFKVVEYK SQVVAGQILF
     MKVDVGNGRF LHMKVLRGLS GDDDLKLLDY QTNKTKNDEL TDF