ID   CYTA_SARPE              Reviewed;         122 AA.
AC   P31727;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   25-MAY-2022, entry version 66.
DE   RecName: Full=Sarcocystatin-A;
DE   Flags: Precursor;
OS   Sarcophaga peregrina (Flesh fly) (Boettcherisca peregrina).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Oestroidea;
OC   Sarcophagidae; Sarcophaga; Boettcherisca.
OX   NCBI_TaxID=7386;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 22-27 AND 61-86, AND
RP   PYROGLUTAMATE FORMATION AT GLN-21.
RC   TISSUE=Hemolymph;
RX   PubMed=2785815; DOI=10.1021/bi00430a049;
RA   Saito H., Suzuki T., Ueno K., Kubo T., Natori S.;
RT   "Molecular cloning of cDNA for sarcocystatin A and analysis of the
RT   expression of the sarcocystatin A gene during development of Sarcophaga
RT   peregrina.";
RL   Biochemistry 28:1749-1755(1989).
CC   -!- FUNCTION: Selectively inhibits the activity of cysteine proteinase of
CC       hemocytes, protecting developing adult tissue in pupae from attack by
CC       the proteinase.
CC   -!- DEVELOPMENTAL STAGE: Expressed at very early embryogenic stage and in
CC       the pupal stage.
CC   -!- SIMILARITY: Belongs to the cystatin family. {ECO:0000305}.
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DR   EMBL; J02847; AAA29985.1; -; mRNA.
DR   PIR; A43644; A43644.
DR   AlphaFoldDB; P31727; -.
DR   SMR; P31727; -.
DR   MEROPS; I25.013; -.
DR   GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   CDD; cd00042; CY; 1.
DR   InterPro; IPR000010; Cystatin_dom.
DR   InterPro; IPR046350; Cystatin_sf.
DR   InterPro; IPR018073; Prot_inh_cystat_CS.
DR   Pfam; PF00031; Cystatin; 1.
DR   SMART; SM00043; CY; 1.
DR   SUPFAM; SSF54403; SSF54403; 1.
DR   PROSITE; PS00287; CYSTATIN; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Protease inhibitor; Pyrrolidone carboxylic acid;
KW   Signal; Thiol protease inhibitor.
FT   SIGNAL          1..20
FT   CHAIN           21..122
FT                   /note="Sarcocystatin-A"
FT                   /id="PRO_0000006670"
FT   MOTIF           67..71
FT                   /note="Secondary area of contact"
FT                   /evidence="ECO:0000250"
FT   SITE            24
FT                   /note="Reactive site"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         21
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000269|PubMed:2785815"
SQ   SEQUENCE   122 AA;  13227 MW;  A0A4166FA69A619A CRC64;
     MKYVLILCVI TLATVAYAQP QCVGCPSEVK GDKLKQSEET LNKSLSKLAA GDGPTYKLVK
     INSATTQVVS GSKDVINADL KDENDKTKTC DITIWSQPWL ENGIEVTFNC PGEPKVVKKH
     SA