CYTB_MOUSE
ID CYTB_MOUSE Reviewed; 98 AA.
AC Q62426; Q3UAW2;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Cystatin-B;
DE AltName: Full=Stefin-B;
GN Name=Cstb; Synonyms=Cst6, Stfb;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8938434; DOI=10.1101/gr.6.11.1103;
RA Pennacchio L.A., Myers R.M.;
RT "Isolation and characterization of the mouse cystatin B gene.";
RL Genome Res. 6:1103-1109(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Bone marrow, and Kidney;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: This is an intracellular thiol proteinase inhibitor.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- TISSUE SPECIFICITY: Widely expressed. Highest expression in heart,
CC liver and kidney. Lower levels in brain, lung and skeletal muscle.
CC Lowest levels in spleen and testis.
CC -!- SIMILARITY: Belongs to the cystatin family. {ECO:0000305}.
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DR EMBL; U59807; AAC52851.1; -; Genomic_DNA.
DR EMBL; AK002403; BAB22074.1; -; mRNA.
DR EMBL; AK151205; BAE30202.1; -; mRNA.
DR EMBL; BC056170; AAH56170.1; -; mRNA.
DR CCDS; CCDS23964.1; -.
DR RefSeq; NP_031819.1; NM_007793.3.
DR AlphaFoldDB; Q62426; -.
DR SMR; Q62426; -.
DR BioGRID; 198959; 15.
DR IntAct; Q62426; 2.
DR MINT; Q62426; -.
DR STRING; 10090.ENSMUSP00000005185; -.
DR MEROPS; I25.003; -.
DR iPTMnet; Q62426; -.
DR PhosphoSitePlus; Q62426; -.
DR SwissPalm; Q62426; -.
DR SWISS-2DPAGE; Q62426; -.
DR EPD; Q62426; -.
DR jPOST; Q62426; -.
DR MaxQB; Q62426; -.
DR PaxDb; Q62426; -.
DR PeptideAtlas; Q62426; -.
DR PRIDE; Q62426; -.
DR ProteomicsDB; 277940; -.
DR Antibodypedia; 4378; 820 antibodies from 38 providers.
DR DNASU; 13014; -.
DR Ensembl; ENSMUST00000005185; ENSMUSP00000005185; ENSMUSG00000005054.
DR GeneID; 13014; -.
DR KEGG; mmu:13014; -.
DR UCSC; uc007fxt.1; mouse.
DR CTD; 1476; -.
DR MGI; MGI:109514; Cstb.
DR VEuPathDB; HostDB:ENSMUSG00000005054; -.
DR eggNOG; ENOG502SF2X; Eukaryota.
DR GeneTree; ENSGT00940000154826; -.
DR HOGENOM; CLU_150234_2_1_1; -.
DR InParanoid; Q62426; -.
DR OMA; AAYQTNK; -.
DR OrthoDB; 1647595at2759; -.
DR PhylomeDB; Q62426; -.
DR TreeFam; TF333174; -.
DR Reactome; R-MMU-6798695; Neutrophil degranulation.
DR BioGRID-ORCS; 13014; 1 hit in 72 CRISPR screens.
DR ChiTaRS; Cstb; mouse.
DR PRO; PR:Q62426; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; Q62426; protein.
DR Bgee; ENSMUSG00000005054; Expressed in gastrula and 278 other tissues.
DR Genevisible; Q62426; MM.
DR GO; GO:0062023; C:collagen-containing extracellular matrix; HDA:BHF-UCL.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005615; C:extracellular space; ISO:MGI.
DR GO; GO:0005730; C:nucleolus; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; ISO:MGI.
DR GO; GO:0004866; F:endopeptidase inhibitor activity; ISO:MGI.
DR GO; GO:0002020; F:protease binding; ISO:MGI.
DR GO; GO:0008344; P:adult locomotory behavior; IMP:MGI.
DR GO; GO:0010466; P:negative regulation of peptidase activity; ISO:MGI.
DR GO; GO:0045861; P:negative regulation of proteolysis; ISO:MGI.
DR CDD; cd00042; CY; 1.
DR InterPro; IPR000010; Cystatin_dom.
DR InterPro; IPR046350; Cystatin_sf.
DR InterPro; IPR018073; Prot_inh_cystat_CS.
DR InterPro; IPR001713; Prot_inh_stefin.
DR PANTHER; PTHR11414; PTHR11414; 1.
DR Pfam; PF00031; Cystatin; 1.
DR PRINTS; PR00295; STEFINA.
DR SMART; SM00043; CY; 1.
DR SUPFAM; SSF54403; SSF54403; 1.
DR PROSITE; PS00287; CYSTATIN; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cytoplasm; Protease inhibitor; Reference proteome;
KW Thiol protease inhibitor.
FT CHAIN 1..98
FT /note="Cystatin-B"
FT /id="PRO_0000207141"
FT MOTIF 46..50
FT /note="Secondary area of contact"
FT /evidence="ECO:0000250"
FT SITE 4
FT /note="Reactive site"
FT /evidence="ECO:0000250"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:P25417"
SQ SEQUENCE 98 AA; 11046 MW; 87A23E6493D28AB8 CRC64;
MMCGAPSATM PATAETQEVA DQVKSQLESK ENQKFDVFKA ISFKRQIVAG TNLFIKVDVG
GDKCVHLRVF QPLPHENKPL TLSSYQTNKE RHDELSYF
