ID   CYTB_RAT                Reviewed;          98 AA.
AC   P01041;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   03-AUG-2022, entry version 150.
DE   RecName: Full=Cystatin-B;
DE   AltName: Full=Cystatin-beta;
DE   AltName: Full=Liver thiol proteinase inhibitor;
DE   AltName: Full=Stefin-B;
GN   Name=Cstb; Synonyms=Cst6;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   PROTEIN SEQUENCE, AND ACETYLATION AT MET-1.
RC   TISSUE=Liver;
RX   PubMed=6626228; DOI=10.1016/s0006-291x(83)80020-5;
RA   Takio K., Kominami E., Wakamatsu N., Katunuma N., Titani K.;
RT   "Amino acid sequence of rat liver thiol proteinase inhibitor.";
RL   Biochem. Biophys. Res. Commun. 115:902-908(1983).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Wistar; TISSUE=Pancreas;
RX   PubMed=2251135; DOI=10.1093/nar/18.22.6698;
RA   Sato N., Ishidoh K., Uchiyama Y., Kominami E.;
RT   "Molecular cloning and sequencing of cDNA for rat cystatin beta.";
RL   Nucleic Acids Res. 18:6698-6698(1990).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1601307; DOI=10.1016/0378-1119(92)90584-c;
RA   Sato N., Ishidoh K., Uchiyama Y., Kominami E.;
RT   "Structural organization of the gene encoding rat cystatin beta.";
RL   Gene 114:257-260(1992).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   PROTEIN SEQUENCE OF 69-89, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC   STRAIN=Sprague-Dawley; TISSUE=Spinal cord;
RA   Lubec G., Afjehi-Sadat L.;
RL   Submitted (NOV-2006) to UniProtKB.
CC   -!- FUNCTION: This is an intracellular thiol proteinase inhibitor.
CC   -!- INTERACTION:
CC       P01041; P00441: SOD1; Xeno; NbExp=3; IntAct=EBI-26602017, EBI-990792;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the cystatin family. {ECO:0000305}.
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DR   EMBL; X54737; CAA38534.1; -; mRNA.
DR   EMBL; D10607; BAA01462.1; -; Genomic_DNA.
DR   EMBL; BC084725; AAH84725.1; -; mRNA.
DR   PIR; JC1128; UDRTS.
DR   RefSeq; NP_036970.1; NM_012838.2.
DR   AlphaFoldDB; P01041; -.
DR   SMR; P01041; -.
DR   IntAct; P01041; 1.
DR   MINT; P01041; -.
DR   STRING; 10116.ENSRNOP00000001593; -.
DR   MEROPS; I25.003; -.
DR   iPTMnet; P01041; -.
DR   PhosphoSitePlus; P01041; -.
DR   jPOST; P01041; -.
DR   PaxDb; P01041; -.
DR   PRIDE; P01041; -.
DR   Ensembl; ENSRNOT00000001593; ENSRNOP00000001593; ENSRNOG00000001201.
DR   GeneID; 25308; -.
DR   KEGG; rno:25308; -.
DR   UCSC; RGD:2435; rat.
DR   CTD; 1476; -.
DR   RGD; 2435; Cstb.
DR   eggNOG; ENOG502SF2X; Eukaryota.
DR   GeneTree; ENSGT00940000154826; -.
DR   HOGENOM; CLU_150234_2_1_1; -.
DR   InParanoid; P01041; -.
DR   OMA; AAYQTNK; -.
DR   OrthoDB; 1647595at2759; -.
DR   PhylomeDB; P01041; -.
DR   TreeFam; TF333174; -.
DR   Reactome; R-RNO-6798695; Neutrophil degranulation.
DR   PRO; PR:P01041; -.
DR   Proteomes; UP000002494; Chromosome 20.
DR   Bgee; ENSRNOG00000001201; Expressed in thymus and 20 other tissues.
DR   Genevisible; P01041; RN.
DR   GO; GO:0005737; C:cytoplasm; ISO:RGD.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005615; C:extracellular space; ISO:RGD.
DR   GO; GO:0005730; C:nucleolus; IEA:Ensembl.
DR   GO; GO:0005634; C:nucleus; ISO:RGD.
DR   GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; ISO:RGD.
DR   GO; GO:0004866; F:endopeptidase inhibitor activity; ISO:RGD.
DR   GO; GO:0002020; F:protease binding; ISO:RGD.
DR   GO; GO:0008344; P:adult locomotory behavior; ISO:RGD.
DR   GO; GO:0010466; P:negative regulation of peptidase activity; ISO:RGD.
DR   GO; GO:0045861; P:negative regulation of proteolysis; ISO:RGD.
DR   CDD; cd00042; CY; 1.
DR   InterPro; IPR000010; Cystatin_dom.
DR   InterPro; IPR046350; Cystatin_sf.
DR   InterPro; IPR018073; Prot_inh_cystat_CS.
DR   InterPro; IPR001713; Prot_inh_stefin.
DR   PANTHER; PTHR11414; PTHR11414; 1.
DR   Pfam; PF00031; Cystatin; 1.
DR   PRINTS; PR00295; STEFINA.
DR   SMART; SM00043; CY; 1.
DR   SUPFAM; SSF54403; SSF54403; 1.
DR   PROSITE; PS00287; CYSTATIN; 1.
PE   1: Evidence at protein level;
KW   Acetylation; Cytoplasm; Direct protein sequencing; Protease inhibitor;
KW   Reference proteome; Thiol protease inhibitor.
FT   CHAIN           1..98
FT                   /note="Cystatin-B"
FT                   /id="PRO_0000207143"
FT   MOTIF           46..50
FT                   /note="Secondary area of contact"
FT   SITE            4
FT                   /note="Reactive site"
FT   MOD_RES         1
FT                   /note="N-acetylmethionine"
FT                   /evidence="ECO:0000269|PubMed:6626228"
SQ   SEQUENCE   98 AA;  11196 MW;  191406A8A5B1A70C CRC64;
     MMCGAPSATM PATTETQEIA DKVKSQLEEK ANQKFDVFKA ISFRRQVVAG TNFFIKVDVG
     EEKCVHLRVF EPLPHENKPL TLSSYQTDKE KHDELTYF