ID   CYTC_BOVIN              Reviewed;         148 AA.
AC   P01035; Q54A26;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1999, sequence version 2.
DT   03-AUG-2022, entry version 151.
DE   RecName: Full=Cystatin-C;
DE   AltName: Full=Colostrum thiol proteinase inhibitor;
DE   AltName: Full=Cystatin-3;
DE   Flags: Precursor;
GN   Name=CST3;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 66-83, PYROGLUTAMATE
RP   FORMATION AT GLN-31, CHARACTERIZATION, AND MASS SPECTROMETRY.
RC   TISSUE=Cerebrospinal fluid, and Choroid plexus;
RX   PubMed=9434110; DOI=10.1016/s0167-4838(97)00110-6;
RA   Olsson S.-L., Ek B., Wilm M., Broberg S., Rask L., Bjoerk I.;
RT   "Molecular cloning and N-terminal analysis of bovine cystatin C
RT   identification of a full-length N-terminal region.";
RL   Biochim. Biophys. Acta 1343:203-210(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=12658628; DOI=10.1002/mrd.10292;
RA   Ishiwata H., Katsuma S., Kizaki K., Patel O.V., Nakano H., Takahashi T.,
RA   Imai K., Hirasawa A., Shiojima S., Ikawa H., Suzuki Y., Tsujimoto G.,
RA   Izaike Y., Todoroki J., Hashizume K.;
RT   "Characterization of gene expression profiles in early bovine pregnancy
RT   using a custom cDNA microarray.";
RL   Mol. Reprod. Dev. 65:9-18(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Crossbred X Angus; TISSUE=Liver;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   PROTEIN SEQUENCE OF 37-148.
RX   PubMed=3891407; DOI=10.1016/0014-5793(85)81335-1;
RA   Hirado M., Tsunasawa S., Sakiyama F., Niinobe M., Fujii S.;
RT   "Complete amino acid sequence of bovine colostrum low-Mr cysteine
RT   proteinase inhibitor.";
RL   FEBS Lett. 186:41-45(1985).
CC   -!- FUNCTION: This is a thiol proteinase inhibitor.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- MASS SPECTROMETRY: Mass=13420; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:9434110};
CC   -!- SIMILARITY: Belongs to the cystatin family. {ECO:0000305}.
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DR   EMBL; Y10811; CAA71771.1; -; mRNA.
DR   EMBL; AB098964; BAC56454.1; -; mRNA.
DR   EMBL; AB099041; BAC56531.1; -; mRNA.
DR   EMBL; BC109629; AAI09630.1; -; mRNA.
DR   PIR; A01271; UDBO.
DR   PIR; S62326; S62326.
DR   RefSeq; NP_776454.1; NM_174029.1.
DR   AlphaFoldDB; P01035; -.
DR   SMR; P01035; -.
DR   STRING; 9913.ENSBTAP00000000790; -.
DR   MEROPS; I25.003; -.
DR   MEROPS; I25.004; -.
DR   PaxDb; P01035; -.
DR   PeptideAtlas; P01035; -.
DR   PRIDE; P01035; -.
DR   Ensembl; ENSBTAT00000000790; ENSBTAP00000000790; ENSBTAG00000000598.
DR   GeneID; 281102; -.
DR   KEGG; bta:281102; -.
DR   CTD; 1471; -.
DR   VEuPathDB; HostDB:ENSBTAG00000000598; -.
DR   eggNOG; ENOG502SC50; Eukaryota.
DR   GeneTree; ENSGT00940000154755; -.
DR   HOGENOM; CLU_118168_0_1_1; -.
DR   InParanoid; P01035; -.
DR   OMA; YTVPWLG; -.
DR   OrthoDB; 1565344at2759; -.
DR   Reactome; R-BTA-381426; Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).
DR   Reactome; R-BTA-6798695; Neutrophil degranulation.
DR   Reactome; R-BTA-8957275; Post-translational protein phosphorylation.
DR   Proteomes; UP000009136; Chromosome 13.
DR   Bgee; ENSBTAG00000000598; Expressed in floor plate of diencephalon and 106 other tissues.
DR   ExpressionAtlas; P01035; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR   GO; GO:0031982; C:vesicle; IBA:GO_Central.
DR   GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IBA:GO_Central.
DR   GO; GO:2000117; P:negative regulation of cysteine-type endopeptidase activity; IBA:GO_Central.
DR   CDD; cd00042; CY; 1.
DR   InterPro; IPR000010; Cystatin_dom.
DR   InterPro; IPR046350; Cystatin_sf.
DR   InterPro; IPR018073; Prot_inh_cystat_CS.
DR   Pfam; PF00031; Cystatin; 1.
DR   SMART; SM00043; CY; 1.
DR   SUPFAM; SSF54403; SSF54403; 1.
DR   PROSITE; PS00287; CYSTATIN; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Protease inhibitor;
KW   Pyrrolidone carboxylic acid; Reference proteome; Secreted; Signal;
KW   Thiol protease inhibitor.
FT   SIGNAL          1..30
FT                   /evidence="ECO:0000305"
FT   CHAIN           31..148
FT                   /note="Cystatin-C"
FT                   /id="PRO_0000006638"
FT   MOTIF           84..88
FT                   /note="Secondary area of contact"
FT   SITE            40
FT                   /note="Reactive site"
FT   MOD_RES         31
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000269|PubMed:9434110"
FT   DISULFID        102..112
FT                   /evidence="ECO:0000250"
FT   DISULFID        126..146
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   148 AA;  16265 MW;  EE740FE37CFB9F0E CRC64;
     MVGSPRAPLL LLASLIVALA LALAVSPAAA QGPRKGRLLG GLMEADVNEE GVQEALSFAV
     SEFNKRSNDA YQSRVVRVVR ARKQVVSGMN YFLDVELGRT TCTKSQANLD SCPFHNQPHL
     KREKLCSFQV YVVPWMNTIN LVKFSCQD