CYTF_MOUSE
ID CYTF_MOUSE Reviewed; 144 AA.
AC O89098;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Cystatin-F;
DE AltName: Full=Cystatin-7;
DE AltName: Full=Cystatin-like metastasis-associated protein;
DE Short=CMAP;
DE AltName: Full=Leukocystatin;
DE Flags: Precursor;
GN Name=Cst7;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RX PubMed=9632704; DOI=10.1074/jbc.273.26.16400;
RA Halfon S., Ford J., Foster J., Dowling L., Lucian L., Sterling M., Xu Y.,
RA Weiss M., Ikeda M., Liggett D., Helms A., Caux C., Lebecque S., Hannum C.,
RA Menon S., McClanahan T., Gorman D., Zurawski G.;
RT "Leukocystatin, a new class II cystatin expressed selectively by
RT hematopoietic cells.";
RL J. Biol. Chem. 273:16400-16408(1998).
CC -!- FUNCTION: Inhibits papain and cathepsin L but with affinities lower
CC than other cystatins. May play a role in immune regulation through
CC inhibition of a unique target in the hematopoietic system.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the cystatin family. {ECO:0000305}.
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DR EMBL; AF031826; AAC40140.1; -; Genomic_DNA.
DR EMBL; AF031825; AAC40139.1; -; mRNA.
DR CCDS; CCDS16857.2; -.
DR RefSeq; NP_034107.2; NM_009977.3.
DR AlphaFoldDB; O89098; -.
DR SMR; O89098; -.
DR IntAct; O89098; 1.
DR MINT; O89098; -.
DR STRING; 10090.ENSMUSP00000086606; -.
DR MEROPS; I25.007; -.
DR GlyGen; O89098; 1 site.
DR PhosphoSitePlus; O89098; -.
DR EPD; O89098; -.
DR PaxDb; O89098; -.
DR PRIDE; O89098; -.
DR ProteomicsDB; 279258; -.
DR Antibodypedia; 24951; 182 antibodies from 34 providers.
DR DNASU; 13011; -.
DR Ensembl; ENSMUST00000089200; ENSMUSP00000086606; ENSMUSG00000068129.
DR GeneID; 13011; -.
DR KEGG; mmu:13011; -.
DR UCSC; uc008muc.2; mouse.
DR CTD; 8530; -.
DR MGI; MGI:1298217; Cst7.
DR VEuPathDB; HostDB:ENSMUSG00000068129; -.
DR eggNOG; ENOG502S5CP; Eukaryota.
DR GeneTree; ENSGT00940000160277; -.
DR HOGENOM; CLU_118168_1_0_1; -.
DR InParanoid; O89098; -.
DR OMA; VVPWLQH; -.
DR OrthoDB; 1565344at2759; -.
DR PhylomeDB; O89098; -.
DR BioGRID-ORCS; 13011; 2 hits in 71 CRISPR screens.
DR ChiTaRS; Cst7; mouse.
DR PRO; PR:O89098; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; O89098; protein.
DR Bgee; ENSMUSG00000068129; Expressed in peripheral lymph node and 51 other tissues.
DR Genevisible; O89098; MM.
DR GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR GO; GO:0031410; C:cytoplasmic vesicle; IMP:ARUK-UCL.
DR GO; GO:0005783; C:endoplasmic reticulum; IMP:ARUK-UCL.
DR GO; GO:0005768; C:endosome; ISO:MGI.
DR GO; GO:0005615; C:extracellular space; IDA:MGI.
DR GO; GO:0005794; C:Golgi apparatus; IDA:ARUK-UCL.
DR GO; GO:0005770; C:late endosome; IMP:ARUK-UCL.
DR GO; GO:0005764; C:lysosome; IMP:ARUK-UCL.
DR GO; GO:0005771; C:multivesicular body; ISO:MGI.
DR GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IMP:MGI.
DR GO; GO:0030414; F:peptidase inhibitor activity; IDA:ARUK-UCL.
DR GO; GO:0042803; F:protein homodimerization activity; ISO:MGI.
DR GO; GO:0006955; P:immune response; IEA:InterPro.
DR GO; GO:0097340; P:inhibition of cysteine-type endopeptidase activity; IMP:MGI.
DR GO; GO:1903979; P:negative regulation of microglial cell activation; IMP:MGI.
DR GO; GO:0010466; P:negative regulation of peptidase activity; IDA:ARUK-UCL.
DR GO; GO:0031643; P:positive regulation of myelination; IMP:MGI.
DR CDD; cd00042; CY; 1.
DR InterPro; IPR042886; Cystatin-F.
DR InterPro; IPR000010; Cystatin_dom.
DR InterPro; IPR046350; Cystatin_sf.
DR PANTHER; PTHR47141; PTHR47141; 1.
DR Pfam; PF00031; Cystatin; 1.
DR SMART; SM00043; CY; 1.
DR SUPFAM; SSF54403; SSF54403; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Glycoprotein; Protease inhibitor; Reference proteome;
KW Secreted; Signal; Thiol protease inhibitor.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT CHAIN 19..144
FT /note="Cystatin-F"
FT /id="PRO_0000006647"
FT MOTIF 80..84
FT /note="Secondary area of contact"
FT SITE 36
FT /note="Reactive site"
FT CARBOHYD 61
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 98..109
FT /evidence="ECO:0000250"
FT DISULFID 123..143
FT /evidence="ECO:0000250"
SQ SEQUENCE 144 AA; 16380 MW; B5837334C1B4A89C CRC64;
MWLAILLALC CLTSDTHGAR PPDFCSKDLI SSVKPGFPKT IETNNPGVLK AARHSVEKFN
NCTNDIFLFK ESHVSKALVQ VVKGLKYMLE VKIGRTTCRK TMHHQLDNCD FQTNPALKRT
LYCYSEVWVI PWLHSFEVPV LLCQ
