CYTL_DROME
ID CYTL_DROME Reviewed; 126 AA.
AC P23779; Q8SZN3; Q9VFI2;
DT 01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-1992, sequence version 2.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Cystatin-like protein;
GN Name=Cys; ORFNames=CG8050;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1701398; DOI=10.1016/0014-5793(90)81349-s;
RA Delbridge M.L., Kelly L.E.;
RT "Sequence analysis, and chromosomal localization of a gene encoding a
RT cystatin-like protein from Drosophila melanogaster.";
RL FEBS Lett. 274:141-145(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- SIMILARITY: Belongs to the cystatin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA38963.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; X55178; CAA38963.1; ALT_INIT; Genomic_DNA.
DR EMBL; AE014297; AAF55073.2; -; Genomic_DNA.
DR EMBL; AY113633; AAM29638.1; -; mRNA.
DR EMBL; AY070635; AAL48106.1; -; mRNA.
DR PIR; S12913; S12913.
DR RefSeq; NP_476856.1; NM_057508.4.
DR AlphaFoldDB; P23779; -.
DR SMR; P23779; -.
DR BioGRID; 66842; 4.
DR DIP; DIP-22398N; -.
DR IntAct; P23779; 27.
DR STRING; 7227.FBpp0082439; -.
DR MEROPS; I25.013; -.
DR PaxDb; P23779; -.
DR PRIDE; P23779; -.
DR DNASU; 41767; -.
DR EnsemblMetazoa; FBtr0082980; FBpp0082439; FBgn0004629.
DR GeneID; 41767; -.
DR KEGG; dme:Dmel_CG8050; -.
DR CTD; 41767; -.
DR FlyBase; FBgn0004629; Cys.
DR VEuPathDB; VectorBase:FBgn0004629; -.
DR eggNOG; ENOG502SC50; Eukaryota.
DR GeneTree; ENSGT00540000073849; -.
DR HOGENOM; CLU_162219_0_0_1; -.
DR InParanoid; P23779; -.
DR OMA; NIQITCE; -.
DR OrthoDB; 1565344at2759; -.
DR PhylomeDB; P23779; -.
DR SignaLink; P23779; -.
DR BioGRID-ORCS; 41767; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 41767; -.
DR PRO; PR:P23779; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0004629; Expressed in mouthpart and 44 other tissues.
DR Genevisible; P23779; DM.
DR GO; GO:0005615; C:extracellular space; HDA:FlyBase.
DR GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR GO; GO:0019953; P:sexual reproduction; HEP:FlyBase.
DR CDD; cd00042; CY; 1.
DR InterPro; IPR000010; Cystatin_dom.
DR InterPro; IPR046350; Cystatin_sf.
DR InterPro; IPR018073; Prot_inh_cystat_CS.
DR Pfam; PF00031; Cystatin; 1.
DR SMART; SM00043; CY; 1.
DR SUPFAM; SSF54403; SSF54403; 1.
DR PROSITE; PS00287; CYSTATIN; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Protease inhibitor; Reference proteome;
KW Thiol protease inhibitor.
FT CHAIN 1..126
FT /note="Cystatin-like protein"
FT /id="PRO_0000207163"
FT MOTIF 72..76
FT /note="Secondary area of contact"
FT /evidence="ECO:0000250"
FT SITE 29
FT /note="Reactive site"
FT /evidence="ECO:0000250"
FT DISULFID 94..115
FT /evidence="ECO:0000250"
FT CONFLICT 59
FT /note="P -> H (in Ref. 4; AAL48106)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 126 AA; 13437 MW; 144FA7C42E9B9A73 CRC64;
MNVVKSLCIL GLVLVSLIAT QAADEQVVGG VSQLEGNSRK EALELLDATL AQLATGDGPS
YKAINVTSVT GQVVAGSLNT YEVELDNGSD KKQCTVKIWT QPWLKENGTN IKIKCSGDDG
ELDRTW
