CYTR_ECOLI
ID CYTR_ECOLI Reviewed; 341 AA.
AC P0ACN7; P06964; Q2M8N0;
DT 01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1988, sequence version 1.
DT 25-MAY-2022, entry version 117.
DE RecName: Full=HTH-type transcriptional repressor CytR;
GN Name=cytR; OrderedLocusNames=b3934, JW3905;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=3515317; DOI=10.1093/nar/14.5.2215;
RA Valentin-Hansen P., Larsen J.E.L., Hoejrup P., Short S.A., Barbier C.S.;
RT "Nucleotide sequence of the CytR regulatory gene of E. coli K-12.";
RL Nucleic Acids Res. 14:2215-2228(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=8346018; DOI=10.1093/nar/21.15.3391;
RA Plunkett G. III, Burland V., Daniels D.L., Blattner F.R.;
RT "Analysis of the Escherichia coli genome. III. DNA sequence of the region
RT from 87.2 to 89.2 minutes.";
RL Nucleic Acids Res. 21:3391-3398(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-80.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=2162050; DOI=10.1073/pnas.87.12.4620;
RA Lee E.H., Masai H., Allen G.C. Jr., Kornberg A.;
RT "The priA gene encoding the primosomal replicative n' protein of
RT Escherichia coli.";
RL Proc. Natl. Acad. Sci. U.S.A. 87:4620-4624(1990).
CC -!- FUNCTION: This protein negatively controls the transcription initiation
CC of genes such as deoCABD, udp, and cdd encoding catabolizing enzymes
CC and nupC, nupG, and tsx encoding transporting and pore-forming
CC proteins. Binds cytidine and adenosine as effectors.
CC -!- INTERACTION:
CC P0ACN7; P69797: manX; NbExp=2; IntAct=EBI-1125696, EBI-554089;
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DR EMBL; X03683; CAA27318.1; -; Genomic_DNA.
DR EMBL; L19201; AAB03066.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76916.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77376.1; -; Genomic_DNA.
DR EMBL; M33293; AAA24417.1; -; Genomic_DNA.
DR PIR; A24963; RPECCT.
DR RefSeq; NP_418369.1; NC_000913.3.
DR RefSeq; WP_000644904.1; NZ_STEB01000017.1.
DR PDB; 2LCV; NMR; -; A=1-67.
DR PDBsum; 2LCV; -.
DR AlphaFoldDB; P0ACN7; -.
DR BMRB; P0ACN7; -.
DR SMR; P0ACN7; -.
DR BioGRID; 4262650; 18.
DR BioGRID; 852724; 5.
DR DIP; DIP-9390N; -.
DR IntAct; P0ACN7; 6.
DR STRING; 511145.b3934; -.
DR jPOST; P0ACN7; -.
DR PaxDb; P0ACN7; -.
DR PRIDE; P0ACN7; -.
DR EnsemblBacteria; AAC76916; AAC76916; b3934.
DR EnsemblBacteria; BAE77376; BAE77376; BAE77376.
DR GeneID; 66672158; -.
DR GeneID; 948427; -.
DR KEGG; ecj:JW3905; -.
DR KEGG; eco:b3934; -.
DR PATRIC; fig|1411691.4.peg.2771; -.
DR EchoBASE; EB0196; -.
DR eggNOG; COG1609; Bacteria.
DR HOGENOM; CLU_037628_6_0_6; -.
DR InParanoid; P0ACN7; -.
DR OMA; HSEFVFM; -.
DR PhylomeDB; P0ACN7; -.
DR BioCyc; EcoCyc:PD04028; -.
DR EvolutionaryTrace; P0ACN7; -.
DR PRO; PR:P0ACN7; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IDA:EcoCyc.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IMP:EcoCyc.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR CDD; cd01392; HTH_LacI; 1.
DR DisProt; DP02541; -.
DR Gene3D; 1.10.260.40; -; 1.
DR InterPro; IPR000843; HTH_LacI.
DR InterPro; IPR046335; LacI/GalR-like_sensor.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR InterPro; IPR028082; Peripla_BP_I.
DR Pfam; PF00356; LacI; 1.
DR Pfam; PF13377; Peripla_BP_3; 1.
DR SMART; SM00354; HTH_LACI; 1.
DR SUPFAM; SSF47413; SSF47413; 1.
DR SUPFAM; SSF53822; SSF53822; 1.
DR PROSITE; PS00356; HTH_LACI_1; 1.
DR PROSITE; PS50932; HTH_LACI_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..341
FT /note="HTH-type transcriptional repressor CytR"
FT /id="PRO_0000107938"
FT DOMAIN 10..64
FT /note="HTH lacI-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00111"
FT DNA_BIND 12..31
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00111"
FT HELIX 12..18
FT /evidence="ECO:0007829|PDB:2LCV"
FT HELIX 23..29
FT /evidence="ECO:0007829|PDB:2LCV"
FT HELIX 38..50
FT /evidence="ECO:0007829|PDB:2LCV"
SQ SEQUENCE 341 AA; 37820 MW; 0C29D8829AE7062C CRC64;
MKAKKQETAA TMKDVALKAK VSTATVSRAL MNPDKVSQAT RNRVEKAARE VGYLPQPMGR
NVKRNESRTI LVIVPDICDP FFSEIIRGIE VTAANHGYLV LIGDCAHQNQ QEKTFIDLII
TKQIDGMLLL GSRLPFDASI EEQRNLPPMV MANEFAPELE LPTVHIDNLT AAFDAVNYLY
EQGHKRIGCI AGPEEMPLCH YRLQGYVQAL RRCGIMVDPQ YIARGDFTFE AGSKAMQQLL
DLPQPPTAVF CHSDVMALGA LSQAKRQGLK VPEDLSIIGF DNIDLTQFCD PPLTTIAQPR
YEIGREAMLL LLDQMQGQHV GSGSRLMDCE LIIRGSTRAL P
