ID   CYTX_ONCVO              Reviewed;         162 AA.
AC   P22085;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 2.
DT   25-MAY-2022, entry version 90.
DE   RecName: Full=Onchocystatin {ECO:0000303|PubMed:1512269};
DE            Short=OV17 {ECO:0000303|PubMed:12704112};
DE   AltName: Full=Cysteine proteinase inhibitor OV7 {ECO:0000303|PubMed:1512269};
DE   Flags: Precursor;
OS   Onchocerca volvulus.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Spirurina; Spiruromorpha; Filarioidea; Onchocercidae; Onchocerca.
OX   NCBI_TaxID=6282;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL
RP   STAGE, AND DISULFIDE BOND.
RC   STRAIN=Savanna;
RX   PubMed=1512269; DOI=10.1016/s0021-9258(18)41931-x;
RA   Lustigman S., Brotman B., Huima T., Prince A.M., McKerrow J.H.;
RT   "Molecular cloning and characterization of onchocystatin, a cysteine
RT   proteinase inhibitor of Onchocerca volvulus.";
RL   J. Biol. Chem. 267:17339-17346(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 33-162.
RC   STRAIN=Savanna;
RX   PubMed=2052041; DOI=10.1016/0166-6851(91)90028-5;
RA   Lustigman S., Brotman B., Huima T., Prince A.M.;
RT   "Characterization of an Onchocerca volvulus cDNA clone encoding a genus
RT   specific antigen present in infective larvae and adult worms.";
RL   Mol. Biochem. Parasitol. 45:65-76(1991).
RN   [3]
RP   FUNCTION.
RX   PubMed=12704112; DOI=10.1128/iai.71.5.2422-2429.2003;
RA   Schierack P.S., Lucius R., Sonnenburg B., Schilling K., Hartmann S.;
RT   "Parasite-specific immunomodulatory functions of filarial cystatin.";
RL   Infect. Immun. 71:2422-2429(2003).
CC   -!- FUNCTION: Cysteine protease inhibitor which inhibits members of the
CC       peptidase C1 family (PubMed:1512269, PubMed:12704112). In the human
CC       host, inhibits CTSL/cathepsin L and CTSS/cathepsin S and to a lesser
CC       extent CTSB/cathepsin B which may cause defects in antigen processing
CC       and thereby impair antigen-driven T cell proliferation
CC       (PubMed:12704112). {ECO:0000269|PubMed:12704112,
CC       ECO:0000269|PubMed:1512269}.
CC   -!- TISSUE SPECIFICITY: Expressed in the cuticle of L3 and L4 larvae,
CC       female adult, and in the eggshell of developing microfilariae.
CC       {ECO:0000269|PubMed:1512269}.
CC   -!- DEVELOPMENTAL STAGE: Expressed in infective larvae (L3, L4) and adult
CC       stages. {ECO:0000269|PubMed:1512269}.
CC   -!- SIMILARITY: Belongs to the cystatin family. {ECO:0000305}.
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DR   EMBL; M37105; AAA29423.1; -; mRNA.
DR   PIR; A43428; A43428.
DR   AlphaFoldDB; P22085; -.
DR   SMR; P22085; -.
DR   STRING; 6282.P22085; -.
DR   MEROPS; I25.031; -.
DR   EnsemblMetazoa; OVOC7453; OVOC7453; WBGene00244262.
DR   HOGENOM; CLU_141818_0_0_1; -.
DR   OMA; QVTIWEK; -.
DR   Proteomes; UP000024404; Unassembled WGS sequence.
DR   GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IDA:UniProtKB.
DR   CDD; cd00042; CY; 1.
DR   InterPro; IPR000010; Cystatin_dom.
DR   InterPro; IPR046350; Cystatin_sf.
DR   InterPro; IPR018073; Prot_inh_cystat_CS.
DR   Pfam; PF00031; Cystatin; 1.
DR   SMART; SM00043; CY; 1.
DR   SUPFAM; SSF54403; SSF54403; 1.
DR   PROSITE; PS00287; CYSTATIN; 1.
PE   1: Evidence at protein level;
KW   Disulfide bond; Protease inhibitor; Reference proteome; Signal;
KW   Thiol protease inhibitor.
FT   SIGNAL          1..32
FT                   /evidence="ECO:0000255"
FT   CHAIN           33..162
FT                   /note="Onchocystatin"
FT                   /id="PRO_0000006669"
FT   REGION          30..54
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           97..101
FT                   /note="Secondary area of contact"
FT                   /evidence="ECO:0000305"
FT   SITE            53
FT                   /note="Reactive site"
FT                   /evidence="ECO:0000305"
FT   DISULFID        115..128
FT                   /evidence="ECO:0000305|PubMed:1512269"
SQ   SEQUENCE   162 AA;  18415 MW;  71FC98785CB39BED CRC64;
     MLTIKDGTLL IHLLLFSVVA LVQLQGAKSA RAKNPSKMES KTGENQDRPV LLGGWEDRDP
     KDEEILELLP SILMKVNEQS NDEYHLMPIK LLKVSSQVVA GVKYKMDVQV ARSQCKKSSN
     EKVDLTKCKK LEGHPEKVMT LEVWEKPWEN FMRVEILGTK EV