CYVH_VIOBI
ID CYVH_VIOBI Reviewed; 31 AA.
AC P85246;
DT 10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT 10-JUN-2008, sequence version 1.
DT 25-MAY-2022, entry version 25.
DE RecName: Full=Cyclotide vibi-H;
OS Viola biflora (Yellow wood violet).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Malpighiales; Violaceae; Viola.
OX NCBI_TaxID=214529;
RN [1] {ECO:0000305}
RP PROTEIN SEQUENCE, FUNCTION, AND MASS SPECTROMETRY.
RX PubMed=18191970; DOI=10.1016/j.phytochem.2007.10.023;
RA Herrmann A., Burman R., Mylne J.S., Karlsson G., Gullbo J., Craik D.J.,
RA Clark R.J., Goeransson U.;
RT "The alpine violet, Viola biflora, is a rich source of cyclotides with
RT potent cytotoxicity.";
RL Phytochemistry 69:939-952(2008).
CC -!- FUNCTION: Probably participates in a plant defense mechanism. Has
CC cytotoxic activity, active against a human lymphoma cell line with an
CC IC(50) of 1.6 uM. {ECO:0000255|PROSITE-ProRule:PRU00395,
CC ECO:0000269|PubMed:18191970, ECO:0000305}.
CC -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC structurally defines this protein as a knottin.
CC {ECO:0000250|UniProtKB:P82230}.
CC -!- PTM: This is a cyclic peptide. {ECO:0000255|PROSITE-ProRule:PRU00395,
CC ECO:0000269|PubMed:18191970}.
CC -!- MASS SPECTROMETRY: Mass=3272; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:18191970};
CC -!- SIMILARITY: Belongs to the cyclotide family. Bracelet subfamily.
CC {ECO:0000255|PROSITE-ProRule:PRU00395}.
CC -!- CAUTION: This peptide is cyclic. The start position was chosen by
CC similarity to OAK1 (kalata-B1) for which the DNA sequence is known.
CC {ECO:0000305}.
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DR AlphaFoldDB; P85246; -.
DR SMR; P85246; -.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR InterPro; IPR005535; Cyclotide.
DR InterPro; IPR012323; Cyclotide_bracelet_CS.
DR InterPro; IPR036146; Cyclotide_sf.
DR Pfam; PF03784; Cyclotide; 1.
DR PIRSF; PIRSF037891; Cycloviolacin; 1.
DR SUPFAM; SSF57038; SSF57038; 1.
DR PROSITE; PS51052; CYCLOTIDE; 1.
DR PROSITE; PS60008; CYCLOTIDE_BRACELET; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Disulfide bond; Knottin; Plant defense.
FT PEPTIDE 1..31
FT /note="Cyclotide vibi-H"
FT /id="PRO_0000341430"
FT DISULFID 5..21
FT /evidence="ECO:0000250|UniProtKB:P82230,
FT ECO:0000255|PROSITE-ProRule:PRU00395"
FT DISULFID 9..23
FT /evidence="ECO:0000250|UniProtKB:P82230,
FT ECO:0000255|PROSITE-ProRule:PRU00395"
FT DISULFID 14..28
FT /evidence="ECO:0000250|UniProtKB:P82230,
FT ECO:0000255|PROSITE-ProRule:PRU00395"
FT CROSSLNK 1..31
FT /note="Cyclopeptide (Gly-Asn)"
FT /evidence="ECO:0000269|PubMed:18191970"
SQ SEQUENCE 31 AA; 3297 MW; 96367A34E584D4F7 CRC64;
GLLPCAESCV YIPCLTTVIG CSCKSKVCYK N