ID   CZCA_ALCSC              Reviewed;        1063 AA.
AC   P94177;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=Cation efflux system protein CzcA;
GN   Name=czcA;
OS   Alcaligenes sp. (strain CT14).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Alcaligenaceae; Alcaligenes.
OX   NCBI_TaxID=68998;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8829543; DOI=10.1271/bbb.60.699;
RA   Kunito T., Kusano T., Oyaizu H., Senoo K., Kanazawa S., Matsumoto S.;
RT   "Cloning and sequence analysis of czc genes in Alcaligenes sp. strain
RT   CT14.";
RL   Biosci. Biotechnol. Biochem. 60:699-704(1996).
CC   -!- FUNCTION: Has a low cation transport activity for cobalt, it is
CC       essential for the expression of cobalt, zinc, and cadmium resistance.
CC       CzcA and CzcB together would act in zinc efflux nearly as effectively
CC       as the complete CZC efflux system (CzcABC).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the resistance-nodulation-cell division (RND)
CC       (TC 2.A.6) family. {ECO:0000305}.
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DR   EMBL; D67044; BAA11061.1; -; Genomic_DNA.
DR   PIR; JC4700; JC4700.
DR   AlphaFoldDB; P94177; -.
DR   SMR; P94177; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008324; F:cation transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0046686; P:response to cadmium ion; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.2090.10; -; 2.
DR   InterPro; IPR027463; AcrB_DN_DC_subdom.
DR   InterPro; IPR001036; Acrflvin-R.
DR   InterPro; IPR004763; CzcA/CusA/SilA/NccA/HelA/CnrA.
DR   PANTHER; PTHR32063; PTHR32063; 1.
DR   Pfam; PF00873; ACR_tran; 1.
DR   PRINTS; PR00702; ACRIFLAVINRP.
DR   SUPFAM; SSF82714; SSF82714; 2.
DR   TIGRFAMs; TIGR00914; 2A0601; 1.
PE   3: Inferred from homology;
KW   Cadmium resistance; Cell membrane; Cobalt; Membrane; Plasmid;
KW   Transmembrane; Transmembrane helix; Transport; Zinc.
FT   CHAIN           1..1063
FT                   /note="Cation efflux system protein CzcA"
FT                   /id="PRO_0000161819"
FT   TRANSMEM        14..29
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        350..370
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        452..472
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        487..507
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        534..554
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        883..903
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        906..926
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        937..957
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        982..1004
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        1013..1033
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          1040..1063
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1063 AA;  115656 MW;  FBA0F8D56FB6710B CRC64;
     MFERIISFAI QQRWLVLLAV FGMAGLGIFS YNRLPIDAVP DITNVQVQVN TSAPGYSPLE
     TEQRVTYPIE VVMAGLPGLE QTRSLSRYGL SQVTVIFKDG TDVYFARQLV NQRIQEAKDN
     LPEGVVPAMG PISTGLGEIY LWTVEAEEGA RKADGTAYTP TDLREIQDWV VRPQLRNVPG
     VTEINTIGGF NKQYLVAPSL ERLASYGLTL TDVVNALNKN NDNVGAGYIE RRGEQYLVRA
     PGQVASEDDI RNIIVGTAQG QPIRIRDIGD VEIGKELRTG AATENGKEVV LGTVFMLIGE
     NSRAVSKAVD EKVASINRTM PEGVKIVTVY DRTRLVDKAI ATVKKNLLEG AVLVIVILFL
     FLGNIRAALI TATIIPLAML FTFTGMVNYK ISANLMSLGA LDFGIIIDGA VVIVENCVRR
     LAHAQEHHGR PLTRSERFHE VFAAAKEARR PLIFGQLIIM IVYLPIFALT GVEGKMFHPM
     AFTVVLALLG AMILSVTFVP AAVALFIGER VAEKENRLML WAKRRYEPLL EKSLANTAVV
     LTFAAVSIVL CVAIAARLGS EFIPNLNEGD IAIQALRIPG TSLSQSVEMQ KTIETTLKAK
     FPEIERVFAR TGTAEIASDP MPPNISDGYI MLKPEKDWPE PKKTHAELLS AIQEEAGKIP
     GNNYEFSQPI QLRFNELISG VRSDVAVKIF GDDNNVLSET AKKVSAVLQG IPGAQEVKVE
     QTTGLPMLTV KIDREKAARY GLNMSDVQDA VATGVGGRDS GTFFQGDRRF DIVVRLPEAV
     RGEVEALRRL PIPLPKGVDA RTTFIPLSEV ATLEMAPGPN QISRENGKRR IVISANVRGR
     DIGSFVPEAE AAIQSQVKIP AGYWMTWGGT FEQLQSATTR LQVVVPVALL LVFVLLFAMF
     NNIKDGLLVF TGIPFALTGG ILALWIRGIP MSITAAVGFI ALCGVAVLNG LVMLSFIRSL
     REEGHSLDSA VRVGALTRLR PVLMTALVAS LGFVPMAIAT GTGAEVQRPL ATVVIGGILS
     STALTLLVLP VLYRLAHRKD EDAEDTREPV TQTHQPDQGR QPA