ID   CZCC_CUPMC              Reviewed;         418 AA.
AC   P13509; P94141; Q1LAI5; Q44010;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   11-JUL-2006, sequence version 4.
DT   25-MAY-2022, entry version 128.
DE   RecName: Full=Cobalt-zinc-cadmium resistance protein CzcC;
DE   AltName: Full=Cation efflux system protein CzcC;
DE   Flags: Precursor;
GN   Name=czcC; OrderedLocusNames=Rmet_5982;
OS   Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 /
OS   CH34) (Ralstonia metallidurans).
OG   Plasmid pMOL30.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Cupriavidus.
OX   NCBI_TaxID=266264;
RN   [1]
RP   PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2678100; DOI=10.1073/pnas.86.19.7351;
RA   Nies D.H., Nies A., Chu L., Silver S.;
RT   "Expression and nucleotide sequence of a plasmid-determined divalent cation
RT   efflux system from Alcaligenes eutrophus.";
RL   Proc. Natl. Acad. Sci. U.S.A. 86:7351-7355(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Monchy S., van der Lelie D., Vallaeys T., Taghavi S., Benotmane M.,
RA   McCorkle S., Dunn J., Lapidus A., Mergeay M.;
RT   "Sequence and features of the Ralstonia metallidurans CH34 heavy metals
RT   plasmids pMOL28 and pMOL30.";
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43123 / DSM 2839 / NBRC 102507 / CH34;
RX   PubMed=20463976; DOI=10.1371/journal.pone.0010433;
RA   Janssen P.J., Van Houdt R., Moors H., Monsieurs P., Morin N., Michaux A.,
RA   Benotmane M.A., Leys N., Vallaeys T., Lapidus A., Monchy S., Medigue C.,
RA   Taghavi S., McCorkle S., Dunn J., van der Lelie D., Mergeay M.;
RT   "The complete genome sequence of Cupriavidus metallidurans strain CH34, a
RT   master survivalist in harsh and anthropogenic environments.";
RL   PLoS ONE 5:E10433-E10433(2010).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-86, CHARACTERIZATION, INDUCTION, AND
RP   DISCUSSION OF FUNCTION.
RX   PubMed=7766206; DOI=10.1007/bf01569896;
RA   Diels L., Dong Q., van der Lelie D., Baeyens W., Mergeay M.;
RT   "The czc operon of Alcaligenes eutrophus CH34: from resistance mechanism to
RT   the removal of heavy metals.";
RL   J. Ind. Microbiol. 14:142-153(1995).
CC   -!- FUNCTION: CzcC protein appears to modify the specificity of the system,
CC       perhaps by acting on the CzcB protein. When the CzcC protein is added
CC       to CzcA and CzcB, the efflux system gains specificity for cadmium and
CC       cobalt.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000305}.
CC   -!- INDUCTION: By zinc, cadmium and cobalt (zinc being the best and cobalt
CC       being the worst inducer). {ECO:0000269|PubMed:7766206}.
CC   -!- BIOTECHNOLOGY: In the presence of 2 mM Cd(2+) or 4-10 mM Zn(2+) up to
CC       99% of the metal is removed from the culture supernatant and is
CC       sequestered via bioprecipitation. Additionally Cu(2+), Co(2+), Ni(2+),
CC       Pb(2+), Y(3+) and Ge(4+) can also be removed from culture supernatants,
CC       although it is not clear which efflux system is responsible for all
CC       these substrates (PubMed:7766206). {ECO:0000269|PubMed:7766206}.
CC   -!- SIMILARITY: Belongs to the outer membrane factor (OMF) (TC 1.B.17)
CC       family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA50525.2; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; X98451; CAA67082.1; -; Genomic_DNA.
DR   EMBL; CP000354; ABF12841.1; -; Genomic_DNA.
DR   EMBL; X71400; CAA50525.2; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_011514819.1; NC_007971.2.
DR   RefSeq; YP_145593.1; NC_006466.1.
DR   AlphaFoldDB; P13509; -.
DR   SMR; P13509; -.
DR   TCDB; 1.B.17.2.2; the outer membrane factor (omf) family.
DR   EnsemblBacteria; ABF12841; ABF12841; Rmet_5982.
DR   KEGG; rme:Rmet_5982; -.
DR   HOGENOM; CLU_012817_14_3_4; -.
DR   OMA; LMTHVEN; -.
DR   OrthoDB; 1533572at2; -.
DR   Proteomes; UP000002429; Plasmid pMOL30.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0015562; F:efflux transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0046686; P:response to cadmium ion; IEA:UniProtKB-KW.
DR   InterPro; IPR003423; OMP_efflux.
DR   Pfam; PF02321; OEP; 2.
PE   1: Evidence at protein level;
KW   Cadmium resistance; Cell outer membrane; Cobalt; Membrane; Plasmid;
KW   Reference proteome; Signal; Transmembrane; Transmembrane beta strand;
KW   Transport; Zinc.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..418
FT                   /note="Cobalt-zinc-cadmium resistance protein CzcC"
FT                   /id="PRO_0000021056"
FT   CONFLICT        106..120
FT                   /note="ELGGKRSARINAAER -> RLAASARLVSMPRKG (in Ref. 1)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        174
FT                   /note="Missing (in Ref. 1; CAA67082)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   418 AA;  44807 MW;  0DD60AD3B79817ED CRC64;
     MRRLFLPLGL AVAFLSPNFA VAQSDTGTSM VPVFPREAAG PLTLEAALSL AAGSNFNLSA
     AAKELDSTEG GIMQARVIPN PELKTLVEDT RKSTRTSTAQ MNIPIELGGK RSARINAAER
     TRELAQATLA GVRGDIRAQV IESFFSVLIA QERVKLATGS ADIAARGAQA ASRRVAAGKI
     SPVDETKARV EQANAELELA EATASLQSAR QALTALWGNA SPQFAEAQGN LDALPSRPAP
     ELLQKELENS PLVAASRAEL DRRQALVGVE RSRQYPDLTV SLGAKRDTEA NRNMAVIGVA
     IPLPIFDRNQ GNLYSAIRQA DKAQDEYLAN RISLTRNLLM ASNQLSVSRA SAQTLKQTVL
     PGAEQAFNAA TIGFEAGKFN YLDVLDAQRT LFQARIRYLG VLGQTYQAAT TIDRILGR