CZCD_BACSU
ID CZCD_BACSU Reviewed; 311 AA.
AC O07084; P71023; Q796A4;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Cadmium, cobalt and zinc/H(+)-K(+) antiporter;
GN Name=czcD; Synonyms=yrdO; OrderedLocusNames=BSU26650;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9308178; DOI=10.1099/00221287-143-9-2939;
RA Sorokin A., Bolotin A., Purnelle B., Hilbert H., Lauber J.,
RA Duesterhoeft A., Ehrlich S.D.;
RT "Sequence of the Bacillus subtilis genome region in the vicinity of the lev
RT operon reveals two new extracytoplasmic function RNA polymerase sigma
RT factors SigV and SigZ.";
RL Microbiology 143:2939-2943(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 17-311.
RC STRAIN=168 / JH642;
RX PubMed=9099864; DOI=10.1016/s0378-1119(96)00784-6;
RA Sturr M.G., Ablooglu A.J., Krulwich T.A.;
RT "A Bacillus subtilis locus encoding several gene products affecting
RT transport of cations.";
RL Gene 188:91-94(1997).
RN [4]
RP INVOLVEMENT IN RESISTANCE TO COBALT.
RC STRAIN=BD99 / MS94;
RX PubMed=10735849; DOI=10.1128/jb.182.8.2088-2095.2000;
RA Wang W., Guffanti A.A., Wei Y., Ito M., Krulwich T.A.;
RT "Two types of Bacillus subtilis tetA(L) deletion strains reveal the
RT physiological importance of TetA(L) in K(+) acquisition as well as in
RT Na(+), alkali, and tetracycline resistance.";
RL J. Bacteriol. 182:2088-2095(2000).
RN [5]
RP FUNCTION IN DIVALENT CATION EFFLUX.
RC STRAIN=BD99 / MS94;
RX PubMed=12100555; DOI=10.1046/j.1365-2958.2002.02998.x;
RA Guffanti A.A., Wei Y., Rood S.V., Krulwich T.A.;
RT "An antiport mechanism for a member of the cation diffusion facilitator
RT family: divalent cations efflux in exchange for K+ and H+.";
RL Mol. Microbiol. 45:145-153(2002).
RN [6]
RP REPRESSION BY CZRA.
RC STRAIN=168;
RX PubMed=15948947; DOI=10.1111/j.1365-2958.2005.04642.x;
RA Moore C.M., Gaballa A., Hui M., Ye R.W., Helmann J.D.;
RT "Genetic and physiological responses of Bacillus subtilis to metal ion
RT stress.";
RL Mol. Microbiol. 57:27-40(2005).
CC -!- FUNCTION: Involved in divalent cation and potassium homeostasis in the
CC cell. Catalyzes the active efflux of zinc, cadmium and cobalt, in
CC exchange for potassium and H(+) ions. {ECO:0000269|PubMed:12100555}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- INDUCTION: Repressed by CzrA. {ECO:0000269|PubMed:15948947}.
CC -!- SIMILARITY: Belongs to the cation diffusion facilitator (CDF)
CC transporter (TC 2.A.4) family. SLC30A subfamily. {ECO:0000305}.
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DR EMBL; U93876; AAB80907.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB14606.1; -; Genomic_DNA.
DR EMBL; U62055; AAB53029.1; -; Genomic_DNA.
DR PIR; C69612; C69612.
DR RefSeq; NP_390542.1; NC_000964.3.
DR RefSeq; WP_003229873.1; NZ_JNCM01000036.1.
DR AlphaFoldDB; O07084; -.
DR SMR; O07084; -.
DR STRING; 224308.BSU26650; -.
DR TCDB; 2.A.4.1.3; the cation diffusion facilitator (cdf) family.
DR PaxDb; O07084; -.
DR PRIDE; O07084; -.
DR EnsemblBacteria; CAB14606; CAB14606; BSU_26650.
DR GeneID; 937630; -.
DR KEGG; bsu:BSU26650; -.
DR PATRIC; fig|224308.179.peg.2896; -.
DR eggNOG; COG1230; Bacteria.
DR InParanoid; O07084; -.
DR OMA; GHEKMLH; -.
DR PhylomeDB; O07084; -.
DR BioCyc; BSUB:BSU26650-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005385; F:zinc ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0006824; P:cobalt ion transport; IEA:UniProtKB-KW.
DR GO; GO:0006813; P:potassium ion transport; IEA:UniProtKB-KW.
DR GO; GO:0071577; P:zinc ion transmembrane transport; IBA:GO_Central.
DR Gene3D; 1.20.1510.10; -; 1.
DR Gene3D; 3.30.70.1350; -; 1.
DR InterPro; IPR002524; Cation_efflux.
DR InterPro; IPR027470; Cation_efflux_CTD.
DR InterPro; IPR036837; Cation_efflux_CTD_sf.
DR InterPro; IPR027469; Cation_efflux_TMD_sf.
DR Pfam; PF01545; Cation_efflux; 1.
DR Pfam; PF16916; ZT_dimer; 1.
DR SUPFAM; SSF160240; SSF160240; 1.
DR SUPFAM; SSF161111; SSF161111; 1.
DR TIGRFAMs; TIGR01297; CDF; 1.
PE 1: Evidence at protein level;
KW Cadmium; Cell membrane; Cobalt; Cobalt transport; Ion transport; Membrane;
KW Potassium; Potassium transport; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport; Zinc; Zinc transport.
FT CHAIN 1..311
FT /note="Cadmium, cobalt and zinc/H(+)-K(+) antiporter"
FT /id="PRO_0000337067"
FT TOPO_DOM 1..12
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 13..33
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 34..43
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 44..64
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 65..78
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 79..99
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 100..115
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 116..136
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 137..157
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 158..178
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 179..311
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CONFLICT 180
FT /note="D -> N (in Ref. 3; AAB53029)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 311 AA; 34163 MW; 9F495E9DF8E96DB0 CRC64;
MGHNHNEGAN KKVLLISFIM ITGYMIIEAI GGFLTNSLAL LSDAGHMLSD SISLMVALIA
FTLAEKKANH NKTFGYKRFE ILAAVINGAA LILISLYIIY EAIERFSNPP KVATTGMLTI
SIIGLVVNLL VAWIMMSGGD TKNNLNIRGA YLHVISDMLG SVGAILAAIL IIFFGWGWAD
PLASIIVAIL VLRSGYNVTK DSIHILMEGT PENIDVSDII RTIEGTEGIQ NIHDLHIWSI
TSGLNALSCH AVVDDQLTIS ESENILRKIE HELEHKGITH VTIQMETEAH NHDNAILCQP
KMEKQRDHHH H
