ID   CZCN_CUPMC              Reviewed;         216 AA.
AC   P94139; Q1LAI3; Q44008;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   10-MAY-2005, sequence version 2.
DT   25-MAY-2022, entry version 111.
DE   RecName: Full=Cobalt-zinc-cadmium resistance protein CzcN;
DE   AltName: Full=Cation efflux system protein CzcN;
GN   Name=czcN; OrderedLocusNames=Rmet_5984;
OS   Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 /
OS   CH34) (Ralstonia metallidurans).
OG   Plasmid pMOL30.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Cupriavidus.
OX   NCBI_TaxID=266264;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1459958; DOI=10.1128/jb.174.24.8102-8110.1992;
RA   Nies D.H.;
RT   "CzcR and CzcD, gene products affecting regulation of resistance to cobalt,
RT   zinc, and cadmium (czc system) in Alcaligenes eutrophus.";
RL   J. Bacteriol. 174:8102-8110(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43123 / DSM 2839 / NBRC 102507 / CH34;
RX   PubMed=20463976; DOI=10.1371/journal.pone.0010433;
RA   Janssen P.J., Van Houdt R., Moors H., Monsieurs P., Morin N., Michaux A.,
RA   Benotmane M.A., Leys N., Vallaeys T., Lapidus A., Monchy S., Medigue C.,
RA   Taghavi S., McCorkle S., Dunn J., van der Lelie D., Mergeay M.;
RT   "The complete genome sequence of Cupriavidus metallidurans strain CH34, a
RT   master survivalist in harsh and anthropogenic environments.";
RL   PLoS ONE 5:E10433-E10433(2010).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 50-216.
RX   PubMed=9044283; DOI=10.1046/j.1365-2958.1997.d01-1866.x;
RA   van der Lelie D., Schwuchow T., Schwidetzky T., Wuertz S., Baeyens W.,
RA   Mergeay M., Nies D.H.;
RT   "Two-component regulatory system involved in transcriptional control of
RT   heavy-metal homoeostasis in Alcaligenes eutrophus.";
RL   Mol. Microbiol. 23:493-503(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 171-216, IDENTIFICATION, AND
RP   DISCUSSION OF FUNCTION.
RX   PubMed=7766206; DOI=10.1007/bf01569896;
RA   Diels L., Dong Q., van der Lelie D., Baeyens W., Mergeay M.;
RT   "The czc operon of Alcaligenes eutrophus CH34: from resistance mechanism to
RT   the removal of heavy metals.";
RL   J. Ind. Microbiol. 14:142-153(1995).
CC   -!- FUNCTION: Component of the CZC cation-efflux system that confers
CC       resistance to cobalt, zinc and cadmium.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: To A.xylosoxydans NccN. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABF12843.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; X71400; CAA50523.2; -; Genomic_DNA.
DR   EMBL; CP000354; ABF12843.1; ALT_INIT; Genomic_DNA.
DR   EMBL; X98451; CAA67079.1; -; Genomic_DNA.
DR   RefSeq; WP_011229344.1; NC_007971.2.
DR   RefSeq; YP_145591.1; NC_006466.1.
DR   AlphaFoldDB; P94139; -.
DR   SMR; P94139; -.
DR   EnsemblBacteria; ABF12843; ABF12843; Rmet_5984.
DR   KEGG; rme:Rmet_5984; -.
DR   HOGENOM; CLU_1018913_0_0_4; -.
DR   Proteomes; UP000002429; Plasmid pMOL30.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0046686; P:response to cadmium ion; IEA:UniProtKB-KW.
DR   InterPro; IPR007318; Phopholipid_MeTrfase.
DR   Pfam; PF04191; PEMT; 1.
PE   4: Predicted;
KW   Cadmium resistance; Cell inner membrane; Cell membrane; Cobalt; Membrane;
KW   Plasmid; Reference proteome; Transmembrane; Transmembrane helix; Transport;
KW   Zinc.
FT   CHAIN           1..216
FT                   /note="Cobalt-zinc-cadmium resistance protein CzcN"
FT                   /id="PRO_0000079763"
FT   TRANSMEM        27..47
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        50..70
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        116..136
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   216 AA;  23753 MW;  F2429D4145AF2A53 CRC64;
     MTDSSISPSH PAGLSRALDN FLTRHRIGVW RVVVTFVLAS LLFGHSRWDG TWVSPLLLTL
     GMLGVSLATV GRLWCALYIS GRKNNTLVTS GPYSLCRHPL YVCNLLGILG LGAMTESLAV
     TAVLALAFAL MYPAVIRTED RFLASAFPEF AEYARRTPAF FPRLSLYRGE STWTVHVSSF
     QRNIADSVWF LGLSVVVESF DLFHDAGVLR AVVTLA