CZIB_HUMAN
ID CZIB_HUMAN Reviewed; 160 AA.
AC Q9NWV4;
DT 12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=CXXC motif containing zinc binding protein {ECO:0000305};
DE AltName: Full=UPF0587 protein C1orf123;
GN Name=CZIB {ECO:0000312|HGNC:HGNC:26059};
GN Synonyms=C1orf123 {ECO:0000312|HGNC:HGNC:26059};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP PROTEIN SEQUENCE OF 10-26, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Brain, and Cajal-Retzius cell;
RA Lubec G., Afjehi-Sadat L.;
RL Submitted (MAR-2007) to UniProtKB.
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-75, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
RN [9]
RP CRYSTALLIZATION, AND SUBUNIT.
RX PubMed=26919524; DOI=10.1107/s2053230x16002016;
RA Rahaman S.N., Mat Yusop J., Mohamed-Hussein Z.A., Ho K.L., Teh A.H.,
RA Waterman J., Ng C.L.;
RT "Cloning, expression, purification, crystallization and X-ray
RT crystallographic analysis of recombinant human C1ORF123 protein.";
RL Acta Crystallogr. F Struct. Biol. Commun. 72:207-213(2016).
RN [10] {ECO:0007744|PDB:5ZLQ}
RP X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) IN COMPLEX WITH ZINC IONS, SUBUNIT,
RP DOMAIN, AND MUTAGENESIS OF CYS-33; CYS-36; CYS-67 AND CYS-70.
RX PubMed=30260988; DOI=10.1371/journal.pone.0204355;
RA Furukawa Y., Lim C., Tosha T., Yoshida K., Hagai T., Akiyama S.,
RA Watanabe S., Nakagome K., Shiro Y.;
RT "Identification of a novel zinc-binding protein, C1orf123, as an interactor
RT with a heavy metal-associated domain.";
RL PLoS ONE 13:e0204355-e0204355(2018).
RN [11] {ECO:0007744|PDB:5ZRT}
RP X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) IN COMPLEX WITH ZINC IONS, SUBUNIT,
RP AND DOMAIN.
RX PubMed=30280012; DOI=10.7717/peerj.5377;
RA Rahaman S.N., Mat Yusop J., Mohamed-Hussein Z.A., Aizat W.M., Ho K.L.,
RA Teh A.H., Waterman J., Tan B.K., Tan H.L., Li A.Y., Chen E.S., Ng C.L.;
RT "Crystal structure and functional analysis of human C1ORF123.";
RL PeerJ 6:E5377-E5377(2018).
CC -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:26919524,
CC ECO:0000269|PubMed:30260988, ECO:0000269|PubMed:30280012}.
CC -!- INTERACTION:
CC Q9NWV4; O14618: CCS; NbExp=3; IntAct=EBI-724109, EBI-11668690;
CC -!- DOMAIN: The N-terminal and the C-terminal half of the protein have a
CC very similar 3D-structure, suggesting they arose from duplication
CC (PubMed:30280012). Requires a bound zinc ion for normal folding and
CC solubility (PubMed:30260988). {ECO:0000269|PubMed:30260988,
CC ECO:0000269|PubMed:30280012}.
CC -!- SIMILARITY: Belongs to the UPF0587 family. {ECO:0000305}.
CC -!- CAUTION: Was identified as interaction partner for CCS
CC (PubMed:30260988). Only misfolded mutant protein forms that lack part
CC of the zinc-binding sites interact with CCS. The full-length protein
CC does not interact with CCS. Likewise, mutant protein that lacks all
CC four zinc-binding residues does not interact with CCS
CC (PubMed:30260988). {ECO:0000269|PubMed:30260988}.
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DR EMBL; AK000587; BAA91272.1; -; mRNA.
DR EMBL; CR457246; CAG33527.1; -; mRNA.
DR EMBL; AL606760; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC010908; AAH10908.1; -; mRNA.
DR CCDS; CCDS576.1; -.
DR RefSeq; NP_001291688.1; NM_001304759.1.
DR RefSeq; NP_001291689.1; NM_001304760.1.
DR RefSeq; NP_060357.1; NM_017887.2.
DR PDB; 5ZLQ; X-ray; 2.00 A; A=1-160.
DR PDB; 5ZRT; X-ray; 1.90 A; A/B=1-160.
DR PDBsum; 5ZLQ; -.
DR PDBsum; 5ZRT; -.
DR AlphaFoldDB; Q9NWV4; -.
DR SMR; Q9NWV4; -.
DR BioGRID; 120322; 22.
DR IntAct; Q9NWV4; 5.
DR MINT; Q9NWV4; -.
DR STRING; 9606.ENSP00000294360; -.
DR GlyGen; Q9NWV4; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q9NWV4; -.
DR MetOSite; Q9NWV4; -.
DR PhosphoSitePlus; Q9NWV4; -.
DR BioMuta; C1orf123; -.
DR DMDM; 74753033; -.
DR REPRODUCTION-2DPAGE; IPI00016605; -.
DR EPD; Q9NWV4; -.
DR jPOST; Q9NWV4; -.
DR MassIVE; Q9NWV4; -.
DR MaxQB; Q9NWV4; -.
DR PaxDb; Q9NWV4; -.
DR PeptideAtlas; Q9NWV4; -.
DR PRIDE; Q9NWV4; -.
DR ProteomicsDB; 82985; -.
DR Antibodypedia; 52610; 69 antibodies from 11 providers.
DR DNASU; 54987; -.
DR Ensembl; ENST00000294360.5; ENSP00000294360.4; ENSG00000162384.14.
DR GeneID; 54987; -.
DR KEGG; hsa:54987; -.
DR MANE-Select; ENST00000294360.5; ENSP00000294360.4; NM_017887.3; NP_060357.1.
DR UCSC; uc001cvd.4; human.
DR CTD; 54987; -.
DR GeneCards; CZIB; -.
DR HGNC; HGNC:26059; CZIB.
DR HPA; ENSG00000162384; Low tissue specificity.
DR neXtProt; NX_Q9NWV4; -.
DR OpenTargets; ENSG00000162384; -.
DR PharmGKB; PA142672441; -.
DR VEuPathDB; HostDB:ENSG00000162384; -.
DR eggNOG; KOG1296; Eukaryota.
DR GeneTree; ENSGT00390000001523; -.
DR HOGENOM; CLU_114688_1_0_1; -.
DR InParanoid; Q9NWV4; -.
DR OMA; FFMKCKM; -.
DR OrthoDB; 1398309at2759; -.
DR PhylomeDB; Q9NWV4; -.
DR TreeFam; TF105959; -.
DR PathwayCommons; Q9NWV4; -.
DR SignaLink; Q9NWV4; -.
DR BioGRID-ORCS; 54987; 11 hits in 1055 CRISPR screens.
DR ChiTaRS; C1orf123; human.
DR GenomeRNAi; 54987; -.
DR Pharos; Q9NWV4; Tdark.
DR PRO; PR:Q9NWV4; -.
DR Proteomes; UP000005640; Chromosome 1.
DR RNAct; Q9NWV4; protein.
DR Bgee; ENSG00000162384; Expressed in tendon of biceps brachii and 206 other tissues.
DR Genevisible; Q9NWV4; HS.
DR GO; GO:0008270; F:zinc ion binding; IDA:UniProtKB.
DR InterPro; IPR008584; CXXC_Zn-binding_euk.
DR PANTHER; PTHR12857; PTHR12857; 1.
DR Pfam; PF05907; DUF866; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Metal-binding; Phosphoprotein;
KW Reference proteome; Zinc.
FT CHAIN 1..160
FT /note="CXXC motif containing zinc binding protein"
FT /id="PRO_0000264151"
FT BINDING 33
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000269|PubMed:30260988,
FT ECO:0000269|PubMed:30280012, ECO:0007744|PDB:5ZLQ,
FT ECO:0007744|PDB:5ZRT"
FT BINDING 36
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000269|PubMed:30260988,
FT ECO:0000269|PubMed:30280012, ECO:0007744|PDB:5ZLQ,
FT ECO:0007744|PDB:5ZRT"
FT BINDING 67
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000269|PubMed:30260988,
FT ECO:0000269|PubMed:30280012, ECO:0007744|PDB:5ZLQ,
FT ECO:0007744|PDB:5ZRT"
FT BINDING 70
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000269|PubMed:30260988,
FT ECO:0000269|PubMed:30280012, ECO:0007744|PDB:5ZLQ,
FT ECO:0007744|PDB:5ZRT"
FT MOD_RES 75
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MUTAGEN 33
FT /note="C->A: Disrupts protein folding and solubility; when
FT associated with A-36; A-67 and A-70."
FT /evidence="ECO:0000269|PubMed:30260988"
FT MUTAGEN 36
FT /note="C->A: Disrupts protein folding and solubility; when
FT associated with A-33; A-67 and A-70."
FT /evidence="ECO:0000269|PubMed:30260988"
FT MUTAGEN 67
FT /note="C->A: Disrupts protein folding and solubility; when
FT associated with A-33; A-36 and A-70."
FT /evidence="ECO:0000269|PubMed:30260988"
FT MUTAGEN 70
FT /note="C->A: Disrupts protein folding and solubility; when
FT associated with A-33; A-36 and A-67."
FT /evidence="ECO:0000269|PubMed:30260988"
FT STRAND 2..20
FT /evidence="ECO:0007829|PDB:5ZRT"
FT STRAND 27..33
FT /evidence="ECO:0007829|PDB:5ZRT"
FT TURN 34..36
FT /evidence="ECO:0007829|PDB:5ZRT"
FT STRAND 44..46
FT /evidence="ECO:0007829|PDB:5ZRT"
FT HELIX 48..50
FT /evidence="ECO:0007829|PDB:5ZRT"
FT STRAND 60..66
FT /evidence="ECO:0007829|PDB:5ZRT"
FT TURN 68..70
FT /evidence="ECO:0007829|PDB:5ZRT"
FT STRAND 73..79
FT /evidence="ECO:0007829|PDB:5ZRT"
FT HELIX 80..82
FT /evidence="ECO:0007829|PDB:5ZRT"
FT HELIX 88..90
FT /evidence="ECO:0007829|PDB:5ZRT"
FT STRAND 95..111
FT /evidence="ECO:0007829|PDB:5ZRT"
FT STRAND 116..120
FT /evidence="ECO:0007829|PDB:5ZRT"
FT TURN 121..123
FT /evidence="ECO:0007829|PDB:5ZRT"
FT STRAND 126..130
FT /evidence="ECO:0007829|PDB:5ZRT"
FT STRAND 137..141
FT /evidence="ECO:0007829|PDB:5ZRT"
FT TURN 142..145
FT /evidence="ECO:0007829|PDB:5ZRT"
FT STRAND 146..159
FT /evidence="ECO:0007829|PDB:5ZRT"
SQ SEQUENCE 160 AA; 18048 MW; 7CACEEE7CE781CBC CRC64;
MGKIALQLKA TLENITNLRP VGEDFRWYLK MKCGNCGEIS DKWQYIRLMD SVALKGGRGS
ASMVQKCKLC ARENSIEILS STIKPYNAED NENFKTIVEF ECRGLEPVDF QPQAGFAAEG
VESGTAFSDI NLQEKDWTDY DEKAQESVGI YEVTHQFVKC
