CZIB_MOUSE
ID CZIB_MOUSE Reviewed; 160 AA.
AC Q8BHG2; A2A8E3; A2A8E5; Q8C7D6; Q9D1H2; Q9DCH5;
DT 12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=CXXC motif containing zinc binding protein {ECO:0000305};
DE AltName: Full=UPF0587 protein C1orf123 homolog;
GN Name=Czib;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
RC STRAIN=C57BL/6J; TISSUE=Hippocampus, Kidney, Olfactory bulb, and Pancreas;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC STRAIN=FVB/N; TISSUE=Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- SUBUNIT: Monomer. {ECO:0000250|UniProtKB:Q9NWV4}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8BHG2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8BHG2-2; Sequence=VSP_021888;
CC Name=3;
CC IsoId=Q8BHG2-3; Sequence=VSP_021887;
CC -!- DOMAIN: The N-terminal and the C-terminal half of the protein have a
CC very similar 3D-structure, suggesting they arose from duplication.
CC Requires a bound zinc ion for normal folding and solubility.
CC {ECO:0000250|UniProtKB:Q9NWV4}.
CC -!- SIMILARITY: Belongs to the UPF0587 family. {ECO:0000305}.
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DR EMBL; AK002776; BAB22350.1; -; mRNA.
DR EMBL; AK003575; BAB22867.1; -; mRNA.
DR EMBL; AK032590; BAC27937.1; -; mRNA.
DR EMBL; AK050486; BAC34284.1; -; mRNA.
DR EMBL; AK050533; BAC34312.1; -; mRNA.
DR EMBL; AK160674; BAE35952.1; -; mRNA.
DR EMBL; AL611936; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC019215; AAH19215.1; -; mRNA.
DR CCDS; CCDS18442.1; -. [Q8BHG2-3]
DR CCDS; CCDS84769.1; -. [Q8BHG2-1]
DR RefSeq; NP_001334089.1; NM_001347160.1. [Q8BHG2-1]
DR RefSeq; NP_083030.1; NM_028754.2. [Q8BHG2-3]
DR AlphaFoldDB; Q8BHG2; -.
DR SMR; Q8BHG2; -.
DR STRING; 10090.ENSMUSP00000119565; -.
DR iPTMnet; Q8BHG2; -.
DR PhosphoSitePlus; Q8BHG2; -.
DR REPRODUCTION-2DPAGE; Q8BHG2; -.
DR EPD; Q8BHG2; -.
DR jPOST; Q8BHG2; -.
DR MaxQB; Q8BHG2; -.
DR PaxDb; Q8BHG2; -.
DR PeptideAtlas; Q8BHG2; -.
DR PRIDE; Q8BHG2; -.
DR Antibodypedia; 52610; 69 antibodies from 11 providers.
DR Ensembl; ENSMUST00000106727; ENSMUSP00000102338; ENSMUSG00000028608. [Q8BHG2-2]
DR Ensembl; ENSMUST00000125107; ENSMUSP00000119565; ENSMUSG00000028608. [Q8BHG2-3]
DR Ensembl; ENSMUST00000135454; ENSMUSP00000114234; ENSMUSG00000028608. [Q8BHG2-1]
DR GeneID; 74098; -.
DR KEGG; mmu:74098; -.
DR UCSC; uc008uak.1; mouse. [Q8BHG2-2]
DR UCSC; uc008ual.1; mouse. [Q8BHG2-1]
DR UCSC; uc008uam.1; mouse. [Q8BHG2-3]
DR CTD; 54987; -.
DR MGI; MGI:1921348; Czib.
DR VEuPathDB; HostDB:ENSMUSG00000028608; -.
DR eggNOG; KOG1296; Eukaryota.
DR GeneTree; ENSGT00390000001523; -.
DR InParanoid; Q8BHG2; -.
DR OMA; FFMKCKM; -.
DR OrthoDB; 1398309at2759; -.
DR TreeFam; TF105959; -.
DR BioGRID-ORCS; 74098; 2 hits in 57 CRISPR screens.
DR PRO; PR:Q8BHG2; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q8BHG2; protein.
DR Bgee; ENSMUSG00000028608; Expressed in undifferentiated genital tubercle and 254 other tissues.
DR ExpressionAtlas; Q8BHG2; baseline and differential.
DR Genevisible; Q8BHG2; MM.
DR GO; GO:0008270; F:zinc ion binding; ISS:UniProtKB.
DR InterPro; IPR008584; CXXC_Zn-binding_euk.
DR PANTHER; PTHR12857; PTHR12857; 1.
DR Pfam; PF05907; DUF866; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Metal-binding; Phosphoprotein; Reference proteome;
KW Zinc.
FT CHAIN 1..160
FT /note="CXXC motif containing zinc binding protein"
FT /id="PRO_0000264152"
FT BINDING 33
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q9NWV4"
FT BINDING 36
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q9NWV4"
FT BINDING 67
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q9NWV4"
FT BINDING 70
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q9NWV4"
FT MOD_RES 75
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NWV4"
FT VAR_SEQ 1..2
FT /note="MG -> MEGVRLGWRGLSREGRGGAWHAAERTRPCTQRVPPSSQ (in
FT isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_021887"
FT VAR_SEQ 136..160
FT /note="DWTDYDEKAQESVGIFEVTHQFVKC -> AHVFSSYNCFLQEGTQVWENDMI
FT FLVRRPRDGV (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_021888"
FT CONFLICT 25
FT /note="F -> L (in Ref. 1; BAC34312)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 160 AA; 18020 MW; 21329F78F4777F07 CRC64;
MGKIALQLKA TLENVTNLRP VGEDFRWYLK MKCGNCGEIS EKWQYIRLMD SVALKGGRGS
ASMVQKCKLC ARENSIDILS STIKAYNAED NEKFKTIVEF ECRGLEPVDF QPQAGFAADG
VESGTVFSDI NLQEKDWTDY DEKAQESVGI FEVTHQFVKC
