ID   CZOG1_MAIZE             Reviewed;         467 AA.
AC   Q93XP7; Q8S465;
DT   04-JAN-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Cis-zeatin O-glucosyltransferase 1;
DE            Short=cisZOG1;
DE            EC=2.4.1.215;
GN   Name=CISZOG1; ORFNames=Z092E12.11;
OS   Zea mays (Maize).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX   NCBI_TaxID=4577;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], ENZYME ACTIVITY, AND TISSUE SPECIFICITY.
RC   TISSUE=Kernel;
RX   PubMed=11331778; DOI=10.1073/pnas.101128798;
RA   Martin R.C., Mok M.C., Habben J.E., Mok D.W.S.;
RT   "A maize cytokinin gene encoding an O-glucosyltransferase specific to cis-
RT   zeatin.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:5922-5926(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=15489523; DOI=10.1534/genetics.104.034629;
RA   Swigonova Z., Bennetzen J.L., Messing J.;
RT   "Structure and evolution of the r/b chromosomal regions in rice, maize, and
RT   sorghum.";
RL   Genetics 169:891-906(2005).
RN   [3]
RP   TISSUE SPECIFICITY, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX   PubMed=12644686; DOI=10.1104/pp.017210;
RA   Veach Y.K., Martin R.C., Mok D.W.S., Malbeck J., Vankova R., Mok M.C.;
RT   "O-glucosylation of cis-zeatin in maize. Characterization of genes,
RT   enzymes, and endogenous cytokinins.";
RL   Plant Physiol. 131:1374-1380(2003).
CC   -!- FUNCTION: Utilizes UDP-glucose as the sugar donor and catalyzes the
CC       formation of O-beta-D-glucosyl-cis-zeatin from cis-zeatin. May regulate
CC       active versus storage forms of cytokinins and could have an impact on
CC       seed growth.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=cis-zeatin + UDP-alpha-D-glucose = H(+) + O-beta-D-glucosyl-
CC         cis-zeatin + UDP; Xref=Rhea:RHEA:20681, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:29043, ChEBI:CHEBI:46570, ChEBI:CHEBI:58223,
CC         ChEBI:CHEBI:58885; EC=2.4.1.215;
CC         Evidence={ECO:0000269|PubMed:11331778};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=46 uM for cis-zeatin {ECO:0000269|PubMed:12644686};
CC       pH dependence:
CC         Optimum pH is 7.5. {ECO:0000269|PubMed:12644686};
CC   -!- TISSUE SPECIFICITY: Highly expressed in root. Expressed at lower level
CC       in kernel and cob. Weakly expressed in leaves. Weakly or not expressed
CC       in stems. {ECO:0000269|PubMed:11331778, ECO:0000269|PubMed:12644686}.
CC   -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAL75980.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF318075; AAK53551.1; -; mRNA.
DR   EMBL; AF466203; AAL75980.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; NP_001105017.1; NM_001111547.2.
DR   AlphaFoldDB; Q93XP7; -.
DR   SMR; Q93XP7; -.
DR   STRING; 4577.GRMZM2G168474_P01; -.
DR   CAZy; GT1; Glycosyltransferase Family 1.
DR   PaxDb; Q93XP7; -.
DR   PRIDE; Q93XP7; -.
DR   EnsemblPlants; Zm00001eb074330_T001; Zm00001eb074330_P001; Zm00001eb074330.
DR   GeneID; 541881; -.
DR   Gramene; Zm00001eb074330_T001; Zm00001eb074330_P001; Zm00001eb074330.
DR   KEGG; zma:541881; -.
DR   MaizeGDB; 403574; -.
DR   eggNOG; KOG1192; Eukaryota.
DR   HOGENOM; CLU_001724_2_1_1; -.
DR   OMA; SASHKQW; -.
DR   OrthoDB; 508327at2759; -.
DR   BioCyc; MetaCyc:CISZOG1-MON; -.
DR   BRENDA; 2.4.1.215; 6752.
DR   SABIO-RK; Q93XP7; -.
DR   Proteomes; UP000007305; Chromosome 2.
DR   ExpressionAtlas; Q93XP7; baseline and differential.
DR   Genevisible; Q93XP7; ZM.
DR   GO; GO:0050502; F:cis-zeatin O-beta-D-glucosyltransferase activity; IDA:AgBase.
DR   GO; GO:0009690; P:cytokinin metabolic process; TAS:AgBase.
DR   GO; GO:0006486; P:protein glycosylation; IDA:AgBase.
DR   GO; GO:0080036; P:regulation of cytokinin-activated signaling pathway; TAS:AgBase.
DR   GO; GO:0010817; P:regulation of hormone levels; TAS:AgBase.
DR   CDD; cd03784; GT1_Gtf-like; 1.
DR   InterPro; IPR002213; UDP_glucos_trans.
DR   InterPro; IPR035595; UDP_glycos_trans_CS.
DR   Pfam; PF00201; UDPGT; 1.
DR   PROSITE; PS00375; UDPGT; 1.
PE   1: Evidence at protein level;
KW   Glycosyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..467
FT                   /note="Cis-zeatin O-glucosyltransferase 1"
FT                   /id="PRO_0000074167"
SQ   SEQUENCE   467 AA;  51129 MW;  0BC8001B5B157577 CRC64;
     MAVDTMESVA VVAVPFPAQG HLNQLLHLSL LLASRGLSVH YAAPPPHVRQ ARARVHGWDP
     RALGSIRFHD LDVPPYDSPA PDLAAPSPFP NHLMPMFEAF AAAARAPLAA LLQRLSTSYR
     RVAVVFDRLN PFAATEAARL ANADAFGLQC VAISYNVGWL DPGHRLLSDY GLQFLPPDAC
     MSREFVDLVF RMEEEEQGAP VAGLVMNTCR ALEGEFLDVV AAQPPFQGQR FFAVGPLNPL
     LLDADAPTTP PGQARHECLE WLDRQPPESV LYVSFGTTSC LHADQVAELA AALKGSKQRF
     VWVLRDADRA DIYAESGESR HAMFLSEFTR ETEGTGLVIT GWAPQLEILA HGATAAFMSH
     CGWNSTIESL SHGKPVLAWP MHSDQPWDSE LLCKYFKAGL LVRPWEKHAE IVPAQAIQKV
     IEEAMLSDSG MAVRQRAKEL GEAVRASVAD GGNSRKDLDD FIGYITR