ID   CZOG2_MAIZE             Reviewed;         463 AA.
AC   Q8RXA5;
DT   04-JAN-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Cis-zeatin O-glucosyltransferase 2 {ECO:0000303|PubMed:12644686};
DE            Short=cisZOG2 {ECO:0000303|PubMed:12644686};
DE            EC=2.4.1.215 {ECO:0000269|PubMed:12644686};
GN   Name=CISZOG2 {ECO:0000303|PubMed:12644686};
GN   Synonyms=CZOG2 {ECO:0000303|PubMed:12644686};
OS   Zea mays (Maize).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX   NCBI_TaxID=4577;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, TISSUE
RP   SPECIFICITY, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX   PubMed=12644686; DOI=10.1104/pp.017210;
RA   Veach Y.K., Martin R.C., Mok D.W.S., Malbeck J., Vankova R., Mok M.C.;
RT   "O-glucosylation of cis-zeatin in maize. Characterization of genes,
RT   enzymes, and endogenous cytokinins.";
RL   Plant Physiol. 131:1374-1380(2003).
CC   -!- FUNCTION: Utilizes UDP-glucose as the sugar donor and catalyzes the
CC       formation of O-beta-D-glucosyl-cis-zeatin from cis-zeatin. May regulate
CC       active versus storage forms of cytokinins and could have an impact on
CC       seed growth. {ECO:0000269|PubMed:12644686}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=cis-zeatin + UDP-alpha-D-glucose = H(+) + O-beta-D-glucosyl-
CC         cis-zeatin + UDP; Xref=Rhea:RHEA:20681, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:29043, ChEBI:CHEBI:46570, ChEBI:CHEBI:58223,
CC         ChEBI:CHEBI:58885; EC=2.4.1.215;
CC         Evidence={ECO:0000269|PubMed:12644686};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=96 uM for cis-zeatin {ECO:0000269|PubMed:12644686};
CC       pH dependence:
CC         Optimum pH is 7.5. {ECO:0000269|PubMed:12644686};
CC   -!- TISSUE SPECIFICITY: Highly expressed in root. Expressed at much lower
CC       level in kernel. Weakly or not expressed in expressed in stems and
CC       leaves. {ECO:0000269|PubMed:12644686}.
CC   -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; AY082660; AAL92460.1; -; Genomic_DNA.
DR   RefSeq; XP_008657045.1; XM_008658823.1.
DR   AlphaFoldDB; Q8RXA5; -.
DR   SMR; Q8RXA5; -.
DR   STRING; 4577.GRMZM2G110511_P01; -.
DR   CAZy; GT1; Glycosyltransferase Family 1.
DR   PaxDb; Q8RXA5; -.
DR   PRIDE; Q8RXA5; -.
DR   EnsemblPlants; Zm00001eb367250_T001; Zm00001eb367250_P001; Zm00001eb367250.
DR   GeneID; 103636476; -.
DR   Gramene; Zm00001eb367250_T001; Zm00001eb367250_P001; Zm00001eb367250.
DR   MaizeGDB; 403575; -.
DR   eggNOG; KOG1192; Eukaryota.
DR   HOGENOM; CLU_001724_2_1_1; -.
DR   OMA; HICKNNQ; -.
DR   OrthoDB; 508327at2759; -.
DR   BioCyc; MetaCyc:CISZOG2-MON; -.
DR   SABIO-RK; Q8RXA5; -.
DR   Proteomes; UP000007305; Chromosome 8.
DR   ExpressionAtlas; Q8RXA5; baseline and differential.
DR   Genevisible; Q8RXA5; ZM.
DR   GO; GO:0050502; F:cis-zeatin O-beta-D-glucosyltransferase activity; IDA:AgBase.
DR   GO; GO:0006486; P:protein glycosylation; IDA:AgBase.
DR   GO; GO:0080036; P:regulation of cytokinin-activated signaling pathway; TAS:AgBase.
DR   CDD; cd03784; GT1_Gtf-like; 1.
DR   InterPro; IPR002213; UDP_glucos_trans.
DR   InterPro; IPR035595; UDP_glycos_trans_CS.
DR   Pfam; PF00201; UDPGT; 1.
DR   PROSITE; PS00375; UDPGT; 1.
PE   1: Evidence at protein level;
KW   Glycosyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..463
FT                   /note="Cis-zeatin O-glucosyltransferase 2"
FT                   /id="PRO_0000074168"
SQ   SEQUENCE   463 AA;  50744 MW;  E844A655E718BD17 CRC64;
     MAVDTMESVA VVAVPFPAQG HLNQLLHLSL LLASRGLSVH YAAPPPHVRQ ARARVHGWDP
     RALGSIHFHD LDVPAYDSPA PDLAAPSPFP NHLMPMFEAF AAAARAPLAA LLQRLSTSYR
     RVAVVFDRLN PFAATEAARL ANADAFGLQC VAISYTVGWL DPGHRLLSDY GLQFLPPDDC
     MSREFVDLVF RMEEEEQGAP VAGLVMNTCR ALEGEFLDAV AAQPPFQGQR FFAVGPLNPL
     LLDADARTAP RHECLEWLDR QPPESVLYVS FGTTSCLHAD QVAELAAALK GSKQRFVWVL
     RDADRADIYA ESGDSRHAKF LSEFTRETEG TGLVVTGWAP QLEILAHGAT AAFMSHCGWN
     SIIESLSHGK PVLAWPMHSD QPWDSELLCN YFKAGLLVRP WEKHAEIIPA QAIQKVIEEA
     MLSDSGMAVR QRAKELGEAV RASVADGGNS RKDLDDFIGY ITR