ID   CZS4_CRUCA              Reviewed;          28 AA.
AC   A0A193H2U2;
DT   12-APR-2017, integrated into UniProtKB/Swiss-Prot.
DT   05-OCT-2016, sequence version 1.
DT   25-MAY-2022, entry version 7.
DE   RecName: Full=Cruzioseptin-4;
DE            Short=CZS-4 {ECO:0000303|PubMed:27321580};
DE   Flags: Precursor; Fragment;
OS   Cruziohyla calcarifer (Splendid leaf frog) (Agalychnis calcarifer).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Hyloidea; Hylidae; Phyllomedusinae;
OC   Cruziohyla.
OX   NCBI_TaxID=318249 {ECO:0000312|EMBL:ANN87761.1};
RN   [1] {ECO:0000312|EMBL:ANN87761.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, MASS SPECTROMETRY,
RP   AMIDATION AT GLU-25, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC   TISSUE=Skin secretion {ECO:0000312|EMBL:ANN87761.1};
RX   PubMed=27321580; DOI=10.1016/j.jprot.2016.06.017;
RA   Proano-Bolanos C., Zhou M., Wang L., Coloma L.A., Chen T., Shaw C.;
RT   "Peptidomic approach identifies cruzioseptins, a new family of potent
RT   antimicrobial peptides in the splendid leaf frog, Cruziohyla calcarifer.";
RL   J. Proteomics 146:1-13(2016).
CC   -!- FUNCTION: Has antimicrobial activity.
CC       {ECO:0000250|UniProtKB:A0A193H362}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:27321580}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000305|PubMed:27321580}.
CC   -!- MASS SPECTROMETRY: Mass=2445.37; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:27321580};
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Cruzioseptin subfamily. {ECO:0000305}.
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DR   EMBL; KX065081; ANN87761.1; -; mRNA.
DR   AlphaFoldDB; A0A193H2U2; -.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Amphibian defense peptide; Antimicrobial;
KW   Cleavage on pair of basic residues; Secreted.
FT   PEPTIDE         3..25
FT                   /note="Cruzioseptin-4"
FT                   /evidence="ECO:0000269|PubMed:27321580"
FT                   /id="PRO_0000439467"
FT   PROPEP          27..28
FT                   /evidence="ECO:0000305|PubMed:27321580"
FT                   /id="PRO_0000439468"
FT   MOD_RES         25
FT                   /note="Glutamic acid 1-amide"
FT                   /evidence="ECO:0000305|PubMed:27321580"
FT   NON_TER         1
FT                   /evidence="ECO:0000312|EMBL:ANN87761.1"
SQ   SEQUENCE   28 AA;  3055 MW;  C47E49B3C0343392 CRC64;
     KRGFLDVIKH VGKAALSVVS HLINEGEH