ID   D10_RFVKA               Reviewed;         260 AA.
AC   P32097; Q77PB7;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   03-AUG-2022, entry version 79.
DE   RecName: Full=mRNA-decapping protein D10;
DE            EC=3.1.3.-;
GN   ORFNames=D10R, s085R;
OS   Rabbit fibroma virus (strain Kasza) (RFV) (Shope fibroma virus (strain
OS   Kasza)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Leporipoxvirus.
OX   NCBI_TaxID=10272;
OH   NCBI_TaxID=9986; Oryctolagus cuniculus (Rabbit).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1660196; DOI=10.1016/0042-6822(91)90529-k;
RA   Strayer D.S., Jerng H.H., O'Connor K.;
RT   "Sequence and analysis of a portion of the genomes of Shope fibroma virus
RT   and malignant rabbit fibroma virus that is important for viral replication
RT   in lymphocytes.";
RL   Virology 185:585-595(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1329373; DOI=10.1016/0168-1702(92)90104-h;
RA   Strayer D.S., Jerng H.H.;
RT   "Sequence and analysis of the BamHI 'D' fragment of Shope fibroma virus:
RT   comparison with similar regions of related poxviruses.";
RL   Virus Res. 25:117-132(1992).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=10562495; DOI=10.1006/viro.1999.0002;
RA   Willer D.O., McFadden G., Evans D.H.;
RT   "The complete genome sequence of shope (Rabbit) fibroma virus.";
RL   Virology 264:319-343(1999).
CC   -!- FUNCTION: Decapping enzyme required for the removal of the 5'-end
CC       m7GpppN cap tethered to viral and host mRNAs to allow their decay in
CC       cells. May therefore accelerate viral and cellular mRNA turnover to
CC       eliminate competing host mRNAs and allow stage-specific synthesis of
CC       viral proteins. Acceleration of the turnover of cellular transcripts
CC       may even promote the shutoff of host protein synthesis (By similarity).
CC       {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC   -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC   -!- SIMILARITY: Belongs to the Nudix hydrolase family. {ECO:0000305}.
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DR   EMBL; M74532; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AF170722; AAF17969.1; -; Genomic_DNA.
DR   RefSeq; NP_051974.1; NC_001266.1.
DR   SMR; P32097; -.
DR   PRIDE; P32097; -.
DR   GeneID; 1486930; -.
DR   KEGG; vg:1486930; -.
DR   Proteomes; UP000000868; Genome.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016791; F:phosphatase activity; IEA:InterPro.
DR   InterPro; IPR015797; NUDIX_hydrolase-like_dom_sf.
DR   InterPro; IPR020084; NUDIX_hydrolase_CS.
DR   InterPro; IPR000086; NUDIX_hydrolase_dom.
DR   InterPro; IPR003301; Vaccinia_D10_decapping.
DR   InterPro; IPR013683; Vaccinia_D10_N.
DR   Pfam; PF00293; NUDIX; 1.
DR   Pfam; PF08476; VD10_N; 1.
DR   PRINTS; PR01364; VD10PROTEIN.
DR   SUPFAM; SSF55811; SSF55811; 1.
DR   PROSITE; PS51462; NUDIX; 1.
DR   PROSITE; PS00893; NUDIX_BOX; 1.
PE   2: Evidence at transcript level;
KW   Hydrolase; Magnesium; Manganese; Metal-binding; Reference proteome.
FT   CHAIN           1..260
FT                   /note="mRNA-decapping protein D10"
FT                   /id="PRO_0000057096"
FT   DOMAIN          46..230
FT                   /note="Nudix hydrolase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00794"
FT   MOTIF           128..149
FT                   /note="Nudix box"
FT   ACT_SITE        143
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   BINDING         134
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         147
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   BINDING         169
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   260 AA;  30460 MW;  ACF5BFCBFD3BAB54 CRC64;
     MNERLQYSHL MNYITKHNRK LSKTYTWNDD SQRVSATGFS NQRLRWSNKT SICLILSTLD
     NKFIACSRKH SFLYSEIVRC RSVFRKKRLF LTYTRFLKKK ERPFLSSKLN VPLDDPGTEH
     NDIIFPGGLP KNEEDPIMCL SREIKEEINI DSKDIYIDSR FFVHLFIEDL LSNRVYETIL
     FLGNTTLTSN EILNNFLANR EIKSLVFLDA LEKGLMCDVL RYVLAISQLK CFGSTGDKTE
     LLYDKVTESE KKMAPRYGFD