ID   D1A_ILLWI               Reviewed;          42 AA.
AC   P0C1R3;
DT   25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   25-JUL-2006, sequence version 1.
DT   25-MAY-2022, entry version 39.
DE   RecName: Full=Delta-hexatoxin-Iw1a {ECO:0000305};
DE            Short=Delta-HXTX-Iw1a {ECO:0000305};
DE   AltName: Full=Delta-atracotoxin-Hs20.1a {ECO:0000303|PubMed:15066415};
DE            Short=Delta-ACTX-Hs20.1a {ECO:0000303|PubMed:15066415};
OS   Illawarra wisharti (Illawarra funnel-web spider).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Mygalomorphae; Hexathelidae; Hadronyche; unclassified Hadronyche.
OX   NCBI_TaxID=278061;
RN   [1]
RP   PROTEIN SEQUENCE, AND SUBCELLULAR LOCATION.
RC   TISSUE=Venom;
RX   PubMed=15066415; DOI=10.1016/j.toxicon.2004.02.006;
RA   Nicholson G.M., Little M.J., Birinyi-Strachan L.C.;
RT   "Structure and function of delta-atracotoxins: lethal neurotoxins targeting
RT   the voltage-gated sodium channel.";
RL   Toxicon 43:587-599(2004).
CC   -!- FUNCTION: Inhibits tetrodotoxin-sensitive sodium channels by binding to
CC       site 3. It slows the inactivation, causes a prolongation of action
CC       potential duration resulting in repetitive firing in autonomic and
CC       motor nerve fibers. Does not depolarize the resting potential. Does not
CC       affect tetrodotoxin-resistant sodium channels. This lethal neurotoxin
CC       is active on both insect and mammalian voltage-gated sodium channels
CC       (Nav). {ECO:0000269|PubMed:15066415}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:15066415}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:15066415}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin.
CC       {ECO:0000250|UniProtKB:P01478}.
CC   -!- SIMILARITY: Belongs to the neurotoxin 06 (delta-actx) family.
CC       {ECO:0000305}.
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DR   AlphaFoldDB; P0C1R3; -.
DR   ArachnoServer; AS000306; delta-hexatoxin-Iw1a.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0019871; F:sodium channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR008017; Atracotoxin_delta.
DR   Pfam; PF05353; Atracotoxin; 1.
DR   PROSITE; PS60018; DELTA_ACTX; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW   Knottin; Neurotoxin; Secreted; Toxin;
KW   Voltage-gated sodium channel impairing toxin.
FT   CHAIN           1..42
FT                   /note="Delta-hexatoxin-Iw1a"
FT                   /evidence="ECO:0000269|PubMed:15066415"
FT                   /id="PRO_0000247526"
FT   DISULFID        1..15
FT                   /evidence="ECO:0000250|UniProtKB:P01478"
FT   DISULFID        8..20
FT                   /evidence="ECO:0000250|UniProtKB:P01478"
FT   DISULFID        14..31
FT                   /evidence="ECO:0000250|UniProtKB:P01478"
FT   DISULFID        16..42
FT                   /evidence="ECO:0000250|UniProtKB:P01478"
SQ   SEQUENCE   42 AA;  4843 MW;  093A2ED89540E284 CRC64;
     CAKKRNWCGK NEDCCCPMKC IYAWYNQQGS CQSTITGLFK KC