D27_ARATH
ID D27_ARATH Reviewed; 264 AA.
AC Q7XA78;
DT 03-APR-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=Beta-carotene isomerase D27, chloroplastic;
DE EC=5.2.1.14;
DE AltName: Full=Protein DWARF-27 homolog;
DE Short=AtD27;
DE Flags: Precursor;
GN Name=D27; OrderedLocusNames=At1g03055; ORFNames=F10O3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=22623516; DOI=10.1104/pp.112.196253;
RA Waters M.T., Brewer P.B., Bussell J.D., Smith S.M., Beveridge C.A.;
RT "The Arabidopsis ortholog of rice DWARF27 acts upstream of MAX1 in the
RT control of plant development by strigolactones.";
RL Plant Physiol. 159:1073-1085(2012).
RN [5]
RP FUNCTION, AND CATALYTIC ACTIVITY.
RX PubMed=22422982; DOI=10.1126/science.1218094;
RA Alder A., Jamil M., Marzorati M., Bruno M., Vermathen M., Bigler P.,
RA Ghisla S., Bouwmeester H., Beyer P., Al-Babili S.;
RT "The path from beta-carotene to carlactone, a strigolactone-like plant
RT hormone.";
RL Science 335:1348-1351(2012).
CC -!- FUNCTION: Involved in strigolactones biosynthesis by catalyzing the
CC isomerization of the C9-C10 double bond in all-trans-beta-carotene
CC leading to 9-cis-beta-carotene and providing the substrate for CCD7.
CC Strigolactones are hormones that inhibit tillering and shoot branching
CC through the MAX-dependent pathway, contribute to the regulation of
CC shoot architectural response to phosphate-limiting conditions and
CC function as rhizosphere signal that stimulates hyphal branching of
CC arbuscular mycorrhizal fungi and trigger seed germination of root
CC parasitic weeds. {ECO:0000269|PubMed:22422982,
CC ECO:0000269|PubMed:22623516}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=all-trans-beta-carotene = 9-cis-beta-carotene;
CC Xref=Rhea:RHEA:34455, ChEBI:CHEBI:17579, ChEBI:CHEBI:67188;
CC EC=5.2.1.14; Evidence={ECO:0000269|PubMed:22422982};
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:22623516}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q7XA78-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Expressed in hypocotyls and shoots. Expressed at
CC low levels in roots. {ECO:0000269|PubMed:22623516}.
CC -!- DISRUPTION PHENOTYPE: Increased shoot branching.
CC {ECO:0000269|PubMed:22623516}.
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DR EMBL; AC006550; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002684; AEE27521.1; -; Genomic_DNA.
DR EMBL; BT010155; AAQ22624.1; -; mRNA.
DR RefSeq; NP_563673.1; NM_100187.3. [Q7XA78-1]
DR AlphaFoldDB; Q7XA78; -.
DR STRING; 3702.AT1G03055.1; -.
DR PaxDb; Q7XA78; -.
DR PRIDE; Q7XA78; -.
DR ProteomicsDB; 222741; -. [Q7XA78-1]
DR EnsemblPlants; AT1G03055.1; AT1G03055.1; AT1G03055. [Q7XA78-1]
DR GeneID; 838334; -.
DR Gramene; AT1G03055.1; AT1G03055.1; AT1G03055. [Q7XA78-1]
DR KEGG; ath:AT1G03055; -.
DR Araport; AT1G03055; -.
DR TAIR; locus:505006097; AT1G03055.
DR eggNOG; ENOG502QSSG; Eukaryota.
DR HOGENOM; CLU_076741_0_1_1; -.
DR InParanoid; Q7XA78; -.
DR OMA; TTIFFAW; -.
DR OrthoDB; 1527389at2759; -.
DR PhylomeDB; Q7XA78; -.
DR PRO; PR:Q7XA78; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q7XA78; baseline and differential.
DR Genevisible; Q7XA78; AT.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0009536; C:plastid; IDA:TAIR.
DR GO; GO:0106365; F:beta-carotene isomerase activity; IEA:UniProtKB-EC.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0010223; P:secondary shoot formation; IMP:TAIR.
DR GO; GO:1901601; P:strigolactone biosynthetic process; IGI:TAIR.
DR InterPro; IPR038938; D27-like.
DR InterPro; IPR025114; D27-like_C.
DR PANTHER; PTHR33591; PTHR33591; 1.
DR Pfam; PF13225; D27-like_C; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Chloroplast; Iron; Isomerase; Metal-binding; Plastid;
KW Reference proteome; Transit peptide.
FT TRANSIT 1..47
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 48..264
FT /note="Beta-carotene isomerase D27, chloroplastic"
FT /id="PRO_0000422051"
SQ SEQUENCE 264 AA; 29795 MW; 50434030D04087BF CRC64;
MNTKLSLSQT KIFTFTTWFN DTRSGLDRRS SISPTLCSKP VYSGKLKAAK ETARIETSNT
KNASIEDSFF SKIAINYLSK NLQDAAGISS SSKSTDYDRL VDTATRVSRN FDTKQQHEFV
LSSLDRALPT VISSLIKMAF PPSKVSRELF ALFTTISFAW LVGPSEVRET EVNGRKEKSV
VYIEKCRFLE QSNCVGMCTH ICKIPSQIFI KNSLGMPIYM EPDFNDLSCK MMFGREPPEI
EDDPAMKQPC FEFCKSNKSY GVKH
