D27_MEDTR
ID D27_MEDTR Reviewed; 252 AA.
AC R4HZ96; A0A396JPB4;
DT 16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT 24-JUL-2013, sequence version 1.
DT 25-MAY-2022, entry version 30.
DE RecName: Full=Beta-carotene isomerase D27, chloroplastic {ECO:0000305};
DE EC=5.2.1.14 {ECO:0000269|PubMed:26503135};
DE AltName: Full=Protein DWARF-27 {ECO:0000305};
DE Flags: Precursor;
GN Name=D27 {ECO:0000303|PubMed:22039214};
GN OrderedLocusNames=MTR_1g471050 {ECO:0000312|EMBL:KEH42623.1};
OS Medicago truncatula (Barrel medic) (Medicago tribuloides).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX NCBI_TaxID=3880;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, AND INDUCTION BY
RP PHOSPHATE DEPRIVATION.
RX PubMed=22039214; DOI=10.1105/tpc.111.089771;
RA Liu W., Kohlen W., Lillo A., Op den Camp R., Ivanov S., Hartog M.,
RA Limpens E., Jamil M., Smaczniak C., Kaufmann K., Yang W.C., Hooiveld G.J.,
RA Charnikhova T., Bouwmeester H.J., Bisseling T., Geurts R.;
RT "Strigolactone biosynthesis in Medicago truncatula and rice requires the
RT symbiotic GRAS-type transcription factors NSP1 and NSP2.";
RL Plant Cell 23:3853-3865(2011).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND IDENTIFICATION.
RC STRAIN=cv. Jemalong A17;
RX PubMed=22089132; DOI=10.1038/nature10625;
RA Young N.D., Debelle F., Oldroyd G.E.D., Geurts R., Cannon S.B.,
RA Udvardi M.K., Benedito V.A., Mayer K.F.X., Gouzy J., Schoof H.,
RA Van de Peer Y., Proost S., Cook D.R., Meyers B.C., Spannagl M., Cheung F.,
RA De Mita S., Krishnakumar V., Gundlach H., Zhou S., Mudge J., Bharti A.K.,
RA Murray J.D., Naoumkina M.A., Rosen B., Silverstein K.A.T., Tang H.,
RA Rombauts S., Zhao P.X., Zhou P., Barbe V., Bardou P., Bechner M.,
RA Bellec A., Berger A., Berges H., Bidwell S., Bisseling T., Choisne N.,
RA Couloux A., Denny R., Deshpande S., Dai X., Doyle J.J., Dudez A.-M.,
RA Farmer A.D., Fouteau S., Franken C., Gibelin C., Gish J., Goldstein S.,
RA Gonzalez A.J., Green P.J., Hallab A., Hartog M., Hua A., Humphray S.J.,
RA Jeong D.-H., Jing Y., Jocker A., Kenton S.M., Kim D.-J., Klee K., Lai H.,
RA Lang C., Lin S., Macmil S.L., Magdelenat G., Matthews L., McCorrison J.,
RA Monaghan E.L., Mun J.-H., Najar F.Z., Nicholson C., Noirot C.,
RA O'Bleness M., Paule C.R., Poulain J., Prion F., Qin B., Qu C., Retzel E.F.,
RA Riddle C., Sallet E., Samain S., Samson N., Sanders I., Saurat O.,
RA Scarpelli C., Schiex T., Segurens B., Severin A.J., Sherrier D.J., Shi R.,
RA Sims S., Singer S.R., Sinharoy S., Sterck L., Viollet A., Wang B.-B.,
RA Wang K., Wang M., Wang X., Warfsmann J., Weissenbach J., White D.D.,
RA White J.D., Wiley G.B., Wincker P., Xing Y., Yang L., Yao Z., Ying F.,
RA Zhai J., Zhou L., Zuber A., Denarie J., Dixon R.A., May G.D.,
RA Schwartz D.C., Rogers J., Quetier F., Town C.D., Roe B.A.;
RT "The Medicago genome provides insight into the evolution of rhizobial
RT symbioses.";
RL Nature 480:520-524(2011).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Jemalong A17;
RX PubMed=24767513; DOI=10.1186/1471-2164-15-312;
RA Tang H., Krishnakumar V., Bidwell S., Rosen B., Chan A., Zhou S.,
RA Gentzbittel L., Childs K.L., Yandell M., Gundlach H., Mayer K.F.,
RA Schwartz D.C., Town C.D.;
RT "An improved genome release (version Mt4.0) for the model legume Medicago
RT truncatula.";
RL BMC Genomics 15:312-312(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Jemalong A17;
RX PubMed=30397259; DOI=10.1038/s41477-018-0286-7;
RA Pecrix Y., Staton S.E., Sallet E., Lelandais-Briere C., Moreau S.,
RA Carrere S., Blein T., Jardinaud M.F., Latrasse D., Zouine M., Zahm M.,
RA Kreplak J., Mayjonade B., Satge C., Perez M., Cauet S., Marande W.,
RA Chantry-Darmon C., Lopez-Roques C., Bouchez O., Berard A., Debelle F.,
RA Munos S., Bendahmane A., Berges H., Niebel A., Buitink J., Frugier F.,
RA Benhamed M., Crespi M., Gouzy J., Gamas P.;
RT "Whole-genome landscape of Medicago truncatula symbiotic genes.";
RL Nat. Plants 4:1017-1025(2018).
RN [5]
RP FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=26503135; DOI=10.1186/s12870-015-0651-x;
RA van Zeijl A., Liu W., Xiao T.T., Kohlen W., Yang W.C., Bisseling T.,
RA Geurts R.;
RT "The strigolactone biosynthesis gene DWARF27 is co-opted in rhizobium
RT symbiosis.";
RL BMC Plant Biol. 15:260-260(2015).
CC -!- FUNCTION: Involved in strigolactones biosynthesis by catalyzing the
CC isomerization of the C9-C10 double bond in all-trans-beta-carotene
CC leading to 9-cis-beta-carotene and providing the substrate for CCD7
CC (PubMed:26503135). Strigolactones are hormones that inhibit tillering
CC and shoot branching through the MAX-dependent pathway, contribute to
CC the regulation of shoot architectural response to phosphate-limiting
CC conditions and function as rhizosphere signals that stimulate hyphal
CC branching of arbuscular mycorrhizal fungi and trigger seed germination
CC of root parasitic weeds (PubMed:26503135).
CC {ECO:0000269|PubMed:26503135}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=all-trans-beta-carotene = 9-cis-beta-carotene;
CC Xref=Rhea:RHEA:34455, ChEBI:CHEBI:17579, ChEBI:CHEBI:67188;
CC EC=5.2.1.14; Evidence={ECO:0000269|PubMed:26503135};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:34456;
CC Evidence={ECO:0000269|PubMed:26503135};
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC Evidence={ECO:0000250|UniProtKB:C7AU21};
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000250|UniProtKB:C7AU21}.
CC -!- TISSUE SPECIFICITY: Highly expressed in roots (PubMed:22039214).
CC Expressed at low levels in leaves and stems (PubMed:22039214).
CC {ECO:0000269|PubMed:22039214}.
CC -!- INDUCTION: Induced by phosphate deprivation (PubMed:22039214,
CC PubMed:26503135). Induced by Rhizobium lipo-chitooligosaccharide
CC elicitors (PubMed:26503135). {ECO:0000269|PubMed:22039214,
CC ECO:0000269|PubMed:26503135}.
CC -!- MISCELLANEOUS: Roots of plants silencing D27 exhibit a strong reduction
CC in strigolactone synthesis. {ECO:0000269|PubMed:26503135}.
CC -!- SEQUENCE CAUTION:
CC Sequence=RHN80129.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; JN629088; AEW07379.1; -; Genomic_DNA.
DR EMBL; CM001217; KEH42623.1; -; Genomic_DNA.
DR EMBL; PSQE01000001; RHN80129.1; ALT_INIT; Genomic_DNA.
DR RefSeq; XP_013468586.1; XM_013613132.1.
DR AlphaFoldDB; R4HZ96; -.
DR EnsemblPlants; KEH42623; KEH42623; MTR_1g471050.
DR GeneID; 25485781; -.
DR Gramene; KEH42623; KEH42623; MTR_1g471050.
DR KEGG; mtr:MTR_1g471050; -.
DR HOGENOM; CLU_076741_0_2_1; -.
DR OrthoDB; 1527389at2759; -.
DR BRENDA; 5.2.1.14; 3201.
DR Proteomes; UP000002051; Chromosome 1.
DR Proteomes; UP000265566; Chromosome 1.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0106365; F:beta-carotene isomerase activity; IEA:UniProtKB-EC.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR InterPro; IPR038938; D27-like.
DR InterPro; IPR025114; D27-like_C.
DR PANTHER; PTHR33591; PTHR33591; 1.
DR Pfam; PF13225; D27-like_C; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Iron; Isomerase; Metal-binding; Plastid; Reference proteome;
KW Transit peptide.
FT TRANSIT 1..43
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 44..252
FT /note="Beta-carotene isomerase D27, chloroplastic"
FT /id="PRO_0000448275"
SQ SEQUENCE 252 AA; 28434 MW; B61F11734CD0CF78 CRC64;
MDSKMIAHNM SLTPTLAQWK KLRLKPKHTF VVGVLARPTD DISEETLRKT NVYKDNWFDK
LAINHLSKSV QAATGISNNK SGFDSLVEAA TVASQKFNTT QQQGIILDAL DRAFPKPILS
VIRRVMPPSK LAREYFAVFT TIFFAWLLGP SEVRESEING RREKNIVHIK KCRFLEETNC
VGMCINLCKM PSQLFIKDSF GMPVNMVPNF DDMSCEMIFG QEPPASTDDP ALKQPCYKLC
KAKKNHATQC LS
