D27_ORYSJ
ID D27_ORYSJ Reviewed; 278 AA.
AC C7AU21; A0A0P0Y3X3; Q2R1Y5;
DT 03-APR-2013, integrated into UniProtKB/Swiss-Prot.
DT 22-SEP-2009, sequence version 1.
DT 03-AUG-2022, entry version 50.
DE RecName: Full=Beta-carotene isomerase D27, chloroplastic {ECO:0000305};
DE EC=5.2.1.14 {ECO:0000269|PubMed:22422982};
DE AltName: Full=Protein DWARF-27 {ECO:0000303|PubMed:19470589};
DE Flags: Precursor;
GN Name=D27; OrderedLocusNames=Os11g0587000, LOC_Os11g37650;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, COFACTOR, SUBCELLULAR
RP LOCATION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=19470589; DOI=10.1105/tpc.109.065987;
RA Lin H., Wang R., Qian Q., Yan M., Meng X., Fu Z., Yan C., Jiang B., Su Z.,
RA Li J., Wang Y.;
RT "DWARF27, an iron-containing protein required for the biosynthesis of
RT strigolactones, regulates rice tiller bud outgrowth.";
RL Plant Cell 21:1512-1525(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16188032; DOI=10.1186/1741-7007-3-20;
RG The rice chromosomes 11 and 12 sequencing consortia;
RT "The sequence of rice chromosomes 11 and 12, rich in disease resistance
RT genes and recent gene duplications.";
RL BMC Biol. 3:20-20(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [7]
RP DISRUPTION PHENOTYPE.
RC STRAIN=cv. Shiokari;
RX PubMed=15659436; DOI=10.1093/pcp/pci022;
RA Ishikawa S., Maekawa M., Arite T., Onishi K., Takamure I., Kyozuka J.;
RT "Suppression of tiller bud activity in tillering dwarf mutants of rice.";
RL Plant Cell Physiol. 46:79-86(2005).
RN [8]
RP DISRUPTION PHENOTYPE.
RC STRAIN=cv. Shiokari;
RX PubMed=17655651; DOI=10.1111/j.1365-313x.2007.03210.x;
RA Arite T., Iwata H., Ohshima K., Maekawa M., Nakajima M., Kojima M.,
RA Sakakibara H., Kyozuka J.;
RT "DWARF10, an RMS1/MAX4/DAD1 ortholog, controls lateral bud outgrowth in
RT rice.";
RL Plant J. 51:1019-1029(2007).
RN [9]
RP FUNCTION, AND CATALYTIC ACTIVITY.
RX PubMed=22422982; DOI=10.1126/science.1218094;
RA Alder A., Jamil M., Marzorati M., Bruno M., Vermathen M., Bigler P.,
RA Ghisla S., Bouwmeester H., Beyer P., Al-Babili S.;
RT "The path from beta-carotene to carlactone, a strigolactone-like plant
RT hormone.";
RL Science 335:1348-1351(2012).
CC -!- FUNCTION: Involved in strigolactones biosynthesis by catalyzing the
CC isomerization of the C9-C10 double bond in all-trans-beta-carotene
CC leading to 9-cis-beta-carotene and providing the substrate for CCD7.
CC Strigolactones are hormones that inhibit tillering and shoot branching
CC through the MAX-dependent pathway, contribute to the regulation of
CC shoot architectural response to phosphate-limiting conditions and
CC function as rhizosphere signals that stimulate hyphal branching of
CC arbuscular mycorrhizal fungi and trigger seed germination of root
CC parasitic weeds. {ECO:0000269|PubMed:19470589,
CC ECO:0000269|PubMed:22422982}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=all-trans-beta-carotene = 9-cis-beta-carotene;
CC Xref=Rhea:RHEA:34455, ChEBI:CHEBI:17579, ChEBI:CHEBI:67188;
CC EC=5.2.1.14; Evidence={ECO:0000269|PubMed:22422982};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:34456;
CC Evidence={ECO:0000269|PubMed:22422982};
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC Evidence={ECO:0000269|PubMed:19470589};
CC Note=Recombinant protein contains about 1.7 mole of iron per mole of
CC protein. {ECO:0000269|PubMed:19470589};
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:19470589}.
CC -!- TISSUE SPECIFICITY: Expressed in young leaves, axillary buds,
CC inflorescence promodia, lateral roots and crown roots. Expressed in
CC vascular cells in the shoot apex of the main stem and young leaves, the
CC nodal vascular anastomosis and large and small vascular bundles of the
CC internodes. {ECO:0000269|PubMed:19470589}.
CC -!- DISRUPTION PHENOTYPE: Increased tillers and reduced plant height,
CC reduced levels of strigolactone. {ECO:0000269|PubMed:15659436,
CC ECO:0000269|PubMed:17655651, ECO:0000269|PubMed:19470589}.
CC -!- MISCELLANEOUS: Recombinant D27 protein expressed in E.coli contains
CC about 1.7 mole of iron per mole of protein.
CC {ECO:0000305|PubMed:19470589}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABA94460.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=EAZ18968.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; FJ641055; ACT91266.1; -; Genomic_DNA.
DR EMBL; DP000010; ABA94460.1; ALT_INIT; Genomic_DNA.
DR EMBL; AP008217; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AP014967; BAT14645.1; -; Genomic_DNA.
DR EMBL; CM000148; EAZ18968.1; ALT_INIT; Genomic_DNA.
DR RefSeq; XP_015615253.1; XM_015759767.1.
DR AlphaFoldDB; C7AU21; -.
DR STRING; 4530.OS11T0587000-01; -.
DR PRIDE; C7AU21; -.
DR EnsemblPlants; Os11t0587000-01; Os11t0587000-01; Os11g0587000.
DR GeneID; 107276001; -.
DR Gramene; Os11t0587000-01; Os11t0587000-01; Os11g0587000.
DR KEGG; osa:107276001; -.
DR eggNOG; ENOG502QSSG; Eukaryota.
DR HOGENOM; CLU_076741_0_0_1; -.
DR OMA; TTIFFAW; -.
DR OrthoDB; 1527389at2759; -.
DR BioCyc; MetaCyc:MON-17611; -.
DR BRENDA; 5.2.1.14; 4460.
DR PlantReactome; R-OSA-5367729; Strigolactone biosynthesis.
DR Proteomes; UP000000763; Chromosome 11.
DR Proteomes; UP000007752; Chromosome 11.
DR Proteomes; UP000059680; Chromosome 11.
DR ExpressionAtlas; C7AU21; baseline and differential.
DR Genevisible; C7AU21; OS.
DR GO; GO:0009507; C:chloroplast; IDA:UniProtKB.
DR GO; GO:0106365; F:beta-carotene isomerase activity; IEA:UniProtKB-EC.
DR GO; GO:0016859; F:cis-trans isomerase activity; IDA:UniProtKB.
DR GO; GO:0005506; F:iron ion binding; IDA:UniProtKB.
DR GO; GO:0010223; P:secondary shoot formation; IMP:UniProtKB.
DR GO; GO:1901601; P:strigolactone biosynthetic process; IDA:UniProtKB.
DR InterPro; IPR038938; D27-like.
DR InterPro; IPR025114; D27-like_C.
DR PANTHER; PTHR33591; PTHR33591; 1.
DR Pfam; PF13225; D27-like_C; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Iron; Isomerase; Metal-binding; Plastid; Reference proteome;
KW Transit peptide.
FT TRANSIT 1..40
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 41..278
FT /note="Beta-carotene isomerase D27, chloroplastic"
FT /id="PRO_0000422052"
SQ SEQUENCE 278 AA; 30759 MW; B82A9C68CDBC8F2F CRC64;
METTTLVLLL PHGGAGGVRP AAAATAKRSY VMRRCCSTVR AVMARPQEAP ASAPAKKTET
AAMMSTVQTE TAAAPPATVY RDSWFDKLAI GYLSRNLQEA SGLKNEKDGY ESLIDAALAI
SRIFSLDKQS EIVTQALERA LPSYILTMIK VMMPPSRFSR EYFAAFTTIF FPWLVGPCEV
MESEVEGRKE KNVVYIPKCR FLESTNCVGM CTNLCKIPCQ KFIQDSLGMK VYMSPNFEDM
SCEMIFGQQP PEDDPALKQP CFRTKCVAKQ NHGVNCSI
