D2_DICDI
ID D2_DICDI Reviewed; 535 AA.
AC P18142; Q54RI7;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 3.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=cAMP-regulated D2 protein;
DE Flags: Precursor;
GN Name=D2; ORFNames=DDB_G0283085;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=AX3;
RX PubMed=2307702; DOI=10.1083/jcb.110.3.669;
RA Bomblies L., Biegelmann E., Doering V., Gerisch G., Krafft-Czepa H.,
RA Noegel A.A., Schleicher M., Humbel B.M.;
RT "Membrane-enclosed crystals in Dictyostelium discoideum cells, consisting
RT of developmentally regulated proteins with sequence similarities to known
RT esterases.";
RL J. Cell Biol. 110:669-679(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP PRELIMINARY PARTIAL NUCLEOTIDE SEQUENCE.
RX PubMed=3031475; DOI=10.1128/mcb.7.1.458-469.1987;
RA Mann S.K.O., Firtel R.A.;
RT "Cyclic AMP regulation of early gene expression in Dictyostelium
RT discoideum: mediation via the cell surface cyclic AMP receptor.";
RL Mol. Cell. Biol. 7:458-469(1987).
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, esterosome membrane.
CC Note=Membrane-enclosed crystals (esterosome).
CC -!- DEVELOPMENTAL STAGE: Crystals accumulates between growth and
CC aggregation stage and disappear only after the spore germination.
CC -!- INDUCTION: Expression is strongly stimulated by periodic pulses of
CC cAMP.
CC -!- SIMILARITY: Belongs to the type-B carboxylesterase/lipase family.
CC {ECO:0000305}.
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DR EMBL; X52465; CAA36703.1; -; Genomic_DNA.
DR EMBL; AAFI02000050; EAL65872.1; -; Genomic_DNA.
DR EMBL; M15966; AAA33182.1; ALT_SEQ; Genomic_DNA.
DR EMBL; M15969; AAA33183.1; ALT_SEQ; Genomic_DNA.
DR EMBL; M15970; AAA33184.1; ALT_SEQ; Genomic_DNA.
DR EMBL; M21592; AAA33185.1; ALT_SEQ; Genomic_DNA.
DR PIR; B34576; B34576.
DR PIR; C26720; C26720.
DR RefSeq; XP_639249.1; XM_634157.1.
DR AlphaFoldDB; P18142; -.
DR SMR; P18142; -.
DR ESTHER; dicdi-crd2p; Cholinesterase-like.
DR PaxDb; P18142; -.
DR EnsemblProtists; EAL65872; EAL65872; DDB_G0283085.
DR GeneID; 8623935; -.
DR KEGG; ddi:DDB_G0283085; -.
DR dictyBase; DDB_G0283085; D2.
DR eggNOG; KOG1516; Eukaryota.
DR HOGENOM; CLU_006586_13_0_1; -.
DR InParanoid; P18142; -.
DR OMA; GAWHGNE; -.
DR PhylomeDB; P18142; -.
DR Reactome; R-DDI-112311; Neurotransmitter clearance.
DR Reactome; R-DDI-1483191; Synthesis of PC.
DR Reactome; R-DDI-211945; Phase I - Functionalization of compounds.
DR Reactome; R-DDI-8964038; LDL clearance.
DR Reactome; R-DDI-9749641; Aspirin ADME.
DR PRO; PR:P18142; -.
DR Proteomes; UP000002195; Chromosome 4.
DR GO; GO:0005938; C:cell cortex; IDA:dictyBase.
DR GO; GO:0033118; C:esterosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0052689; F:carboxylic ester hydrolase activity; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR002018; CarbesteraseB.
DR InterPro; IPR019826; Carboxylesterase_B_AS.
DR InterPro; IPR019819; Carboxylesterase_B_CS.
DR Pfam; PF00135; COesterase; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
DR PROSITE; PS00122; CARBOXYLESTERASE_B_1; 1.
DR PROSITE; PS00941; CARBOXYLESTERASE_B_2; 1.
PE 2: Evidence at transcript level;
KW cAMP; Cytoplasmic vesicle; Disulfide bond; Glycoprotein; Hydrolase;
KW Membrane; Reference proteome; Serine esterase; Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..535
FT /note="cAMP-regulated D2 protein"
FT /id="PRO_0000008617"
FT ACT_SITE 213
FT /note="Acyl-ester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10039"
FT ACT_SITE 338
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 440
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT CARBOHYD 500
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 89..109
FT /evidence="ECO:0000250"
FT DISULFID 265..272
FT /evidence="ECO:0000250"
FT CONFLICT 55
FT /note="V -> I (in Ref. 1; CAA36703)"
FT /evidence="ECO:0000305"
FT CONFLICT 225
FT /note="S -> F (in Ref. 1; CAA36703)"
FT /evidence="ECO:0000305"
FT CONFLICT 487
FT /note="D -> S (in Ref. 1; CAA36703)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 535 AA; 59600 MW; 6B2D2549D13E7782 CRC64;
MNKLLVFILL LLLLINISFA RKRSYIKNTD ASIVVTQFGA IKGIVEDTHR VFYGVPFAQP
PVNQLRWENP IDLKPWENVR ETLTQKSQCA QKCNLGPGVC SPIGTSEDCL YLDVFTPKDA
TPNSKYPVIV YIPGGAFSVG SGSVPLYDAT KFAQSSVIVV NINYRLGVLG FMGTDLMHGN
YGFLDQIKAL EWVYNNIGSF GGNKEMITIW GESAGAFSVS AHLTSTYSRQ YFNAAISSSS
PLTVGLKDKT TARGNANRFA TNVGCNIEDL TCLRGKSMDE ILDAQEKVGL TFGDKILDAF
TIWSPVIDGD IIPMQPLTAV KEGKTYDVPT IIGNVKHEAI PFIYSFFQDS VGIDYYRVLV
AIVFPLNAMK ILPLYPAAPR GQDSRPILSE LITDYLFRCP DRYHTVTNAK KLSSPTYHYH
YVHVKSTGHS LDACDDKVCH GTELSLFFNS YELMGERLDN DEKELAIDIN NYIVNFATTH
NPNTGLDVPV QWRQVTSTQN STLILETTIE TKDTFTNDPK CNALDLTYYR NQVRP
