ID   D42E1_BOVIN             Reviewed;         393 AA.
AC   Q32L94; Q58CX3;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2008, sequence version 2.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Short-chain dehydrogenase/reductase family 42E member 1 {ECO:0000250|UniProtKB:Q8WUS8};
DE            EC=1.1.1.-;
GN   Name=SDR42E1;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=16305752; DOI=10.1186/1471-2164-6-166;
RA   Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L.,
RA   Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.;
RT   "Characterization of 954 bovine full-CDS cDNA sequences.";
RL   BMC Genomics 6:166-166(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Crossbred X Angus; TISSUE=Liver;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the 3-beta-HSD family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAX46671.1; Type=Erroneous termination; Note=Extended C-terminus.; Evidence={ECO:0000305};
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DR   EMBL; BT021824; AAX46671.1; ALT_SEQ; mRNA.
DR   EMBL; BC109694; AAI09695.1; -; mRNA.
DR   RefSeq; NP_001073761.1; NM_001080292.1.
DR   AlphaFoldDB; Q32L94; -.
DR   SMR; Q32L94; -.
DR   STRING; 9913.ENSBTAP00000024475; -.
DR   PaxDb; Q32L94; -.
DR   Ensembl; ENSBTAT00000048714; ENSBTAP00000045705; ENSBTAG00000018394.
DR   GeneID; 532489; -.
DR   KEGG; bta:532489; -.
DR   CTD; 93517; -.
DR   VEuPathDB; HostDB:ENSBTAG00000018394; -.
DR   VGNC; VGNC:34397; SDR42E1.
DR   eggNOG; KOG1430; Eukaryota.
DR   GeneTree; ENSGT00940000158070; -.
DR   InParanoid; Q32L94; -.
DR   OMA; GAYKRSK; -.
DR   OrthoDB; 992332at2759; -.
DR   Proteomes; UP000009136; Chromosome 18.
DR   Bgee; ENSBTAG00000018394; Expressed in liver and 98 other tissues.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0003854; F:3-beta-hydroxy-delta5-steroid dehydrogenase activity; IEA:InterPro.
DR   GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IBA:GO_Central.
DR   GO; GO:0006694; P:steroid biosynthetic process; IEA:InterPro.
DR   InterPro; IPR002225; 3Beta_OHSteriod_DH/Estase.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   Pfam; PF01073; 3Beta_HSD; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   2: Evidence at transcript level;
KW   Membrane; NAD; Oxidoreductase; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..393
FT                   /note="Short-chain dehydrogenase/reductase family 42E
FT                   member 1"
FT                   /id="PRO_0000331753"
FT   TRANSMEM        282..302
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        371..391
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        152
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         156
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        42
FT                   /note="H -> R (in Ref. 1; AAX46671)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        233
FT                   /note="I -> T (in Ref. 2; AAI09695)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        316
FT                   /note="V -> F (in Ref. 1; AAX46671)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        321
FT                   /note="V -> I (in Ref. 1; AAX46671)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        326
FT                   /note="S -> T (in Ref. 1; AAX46671)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   393 AA;  43740 MW;  A739F3CF20E60EF9 CRC64;
     MDSHKSPKET VLITGGGGYF GFRLGCALNL LGVHVILFDI SHPAQTIPEG IRFILGDIRC
     LSDIENAFQG VDVACVFHIA SYGMSGREQL NRSLIEEINV GGTDNILQAC RRRGVPRLVY
     TSTFNVIFGG QVIRNGDESL PYLPLHLHPD HYSRTKSIAE KKVLSANGTA LERGGGVLST
     CALRPAGIYG PGEQRHLPRI VSYIEKGLFR FVYGDPKSLV EFVHVDNLVQ AHILASEALK
     ANKGHIAAGQ PYFISDGRPV NNFEFFRPLV EGLGYKFPST RLPLTLIYCF AFLTEMTHFI
     LGRLYNFQPF LTRTEVYKTG VTHYFSLEKA RKELGYEAQP FDLQEAVEWF KAHGHGRRPG
     SCDSKCLVWD GLVILLVVTV VLVWLLPSVI LSM