D42E1_DANRE
ID D42E1_DANRE Reviewed; 387 AA.
AC A8DZE7; Q32LS5;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 13-NOV-2007, sequence version 1.
DT 03-AUG-2022, entry version 81.
DE RecName: Full=Short-chain dehydrogenase/reductase family 42E member 1 {ECO:0000250|UniProtKB:Q8WUS8};
DE EC=1.1.1.-;
GN Name=sdr42e1; ORFNames=si:ch211-79l17.4, zgc:123280;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=AB; TISSUE=Intestine;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the 3-beta-HSD family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAI09447.1; Type=Miscellaneous discrepancy; Note=Contains miscellaneous N-terminal sequence and a possible frameshift.; Evidence={ECO:0000305};
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DR EMBL; AL929392; CAP09266.1; -; Genomic_DNA.
DR EMBL; BC109446; AAI09447.1; ALT_SEQ; mRNA.
DR RefSeq; NP_001297010.1; NM_001310081.1.
DR AlphaFoldDB; A8DZE7; -.
DR SMR; A8DZE7; -.
DR STRING; 7955.ENSDARP00000005772; -.
DR PaxDb; A8DZE7; -.
DR Ensembl; ENSDART00000169974; ENSDARP00000139728; ENSDARG00000098838.
DR GeneID; 565964; -.
DR KEGG; dre:565964; -.
DR CTD; 93517; -.
DR ZFIN; ZDB-GENE-051120-63; sdr42e1.
DR eggNOG; KOG1430; Eukaryota.
DR GeneTree; ENSGT00940000158070; -.
DR HOGENOM; CLU_007383_6_8_1; -.
DR InParanoid; A8DZE7; -.
DR OMA; GAYKRSK; -.
DR OrthoDB; 992332at2759; -.
DR PhylomeDB; A8DZE7; -.
DR PRO; PR:A8DZE7; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 18.
DR Bgee; ENSDARG00000098838; Expressed in liver and 15 other tissues.
DR ExpressionAtlas; A8DZE7; baseline.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0003854; F:3-beta-hydroxy-delta5-steroid dehydrogenase activity; IEA:InterPro.
DR GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IBA:GO_Central.
DR GO; GO:0006694; P:steroid biosynthetic process; IEA:InterPro.
DR InterPro; IPR002225; 3Beta_OHSteriod_DH/Estase.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR Pfam; PF01073; 3Beta_HSD; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 2: Evidence at transcript level;
KW Membrane; NAD; Oxidoreductase; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..387
FT /note="Short-chain dehydrogenase/reductase family 42E
FT member 1"
FT /id="PRO_0000331757"
FT TRANSMEM 279..299
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 365..385
FT /note="Helical"
FT /evidence="ECO:0000255"
FT ACT_SITE 149
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT BINDING 153
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT CONFLICT 77
FT /note="A -> T (in Ref. 2; AAI09447)"
FT /evidence="ECO:0000305"
FT CONFLICT 203
FT /note="N -> S (in Ref. 2; AAI09447)"
FT /evidence="ECO:0000305"
FT CONFLICT 328
FT /note="R -> Q (in Ref. 2; AAI09447)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 387 AA; 43595 MW; 7E7A467E8F26B92F CRC64;
MEVNRKDNSF LITGGGGYFG FRLACALLKT SSKVVLFDVS PPIQDLPEGL IFMRADIRDY
AQVEKAVRGS HCVFHIASYG MSGREQLNRK LIEEVNVKGT ENILRACVAH SVPRLIYTST
FNVVFGGQEI KNGDESLPYL PLHLHPDHYS RTKSIAEMQV LKANNLALSN STGVLRTCAL
RPAGIYGPGE QRHLPRIVSY IENGIFRFVY GDPDSLVEFV HVDNLVSAHL LAADALTEKQ
QCRAAGQAYF ISDGRPVNNF EFFRPLVEGL GYSFPTLRLP ISMIYFFAFL TEMVHFVVGR
IYNFQPLLTR TEVYKTGVTH YFSMRKAREE LGYEPKLYDL EDVVQWFQAR GHGKKRSRSS
IRKLILDVFV VVAFVAVLLS CLPVVGQ
